High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs

Fungi are a highly diverse group of organisms and play a significant role in decomposition and carbon cycling in boreal ecosystems. To determine how fungal communities are structured in peat bogs and how to obtain representative samples for monitoring fungal community changes, we separately sampled...

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Auteurs principaux:	Martina Vašutová, Martin Jiroušek, Michal Hájek
Format:	article
Langue:	EN
Publié:	Elsevier 2021
Sujets:	Fungi Biodiversity eDNA Peatland Fruitbody Ecology QH540-549.5
Accès en ligne:	https://doaj.org/article/f98f2636f5794ce7b19be1cda2b27362
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spelling	oai:doaj.org-article:f98f2636f5794ce7b19be1cda2b273622021-12-01T04:32:20ZHigh fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs1470-160X10.1016/j.ecolind.2020.107009https://doaj.org/article/f98f2636f5794ce7b19be1cda2b273622021-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X20309481https://doaj.org/toc/1470-160XFungi are a highly diverse group of organisms and play a significant role in decomposition and carbon cycling in boreal ecosystems. To determine how fungal communities are structured in peat bogs and how to obtain representative samples for monitoring fungal community changes, we separately sampled and sequenced (ITS2, Illumina MiSeq) peat, mixed litter and litter from individual dominant plant species in five permanent plots in raised bogs in the Jizerské Hory Mountains (Czech Republic). In total, we detected 68–103 OTUs per plot. The fungal assemblages were mostly influenced by substrate identity, whereas the effect of the site was minimal. Only a few identified OTUs behave like generalists. The most specific fungi were found in dead parts of Calluna vulgaris, Trichophorum cespitosum and Drosera rotundifolia. The high substrate heterogeneity means that sequencing soil core samples does not provide an exhaustive inventory of the fungal diversity in bogs. Poor correspondence was observed between the recorded OTUs and either visually inspected fruitbodies or literature reports on the fungal taxa associated with the analysed substrates.Martina VašutováMartin JiroušekMichal HájekElsevierarticleFungiBiodiversityeDNAPeatlandFruitbodyEcologyQH540-549.5ENEcological Indicators, Vol 121, Iss , Pp 107009- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fungi Biodiversity eDNA Peatland Fruitbody Ecology QH540-549.5
spellingShingle	Fungi Biodiversity eDNA Peatland Fruitbody Ecology QH540-549.5 Martina Vašutová Martin Jiroušek Michal Hájek High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs
description	Fungi are a highly diverse group of organisms and play a significant role in decomposition and carbon cycling in boreal ecosystems. To determine how fungal communities are structured in peat bogs and how to obtain representative samples for monitoring fungal community changes, we separately sampled and sequenced (ITS2, Illumina MiSeq) peat, mixed litter and litter from individual dominant plant species in five permanent plots in raised bogs in the Jizerské Hory Mountains (Czech Republic). In total, we detected 68–103 OTUs per plot. The fungal assemblages were mostly influenced by substrate identity, whereas the effect of the site was minimal. Only a few identified OTUs behave like generalists. The most specific fungi were found in dead parts of Calluna vulgaris, Trichophorum cespitosum and Drosera rotundifolia. The high substrate heterogeneity means that sequencing soil core samples does not provide an exhaustive inventory of the fungal diversity in bogs. Poor correspondence was observed between the recorded OTUs and either visually inspected fruitbodies or literature reports on the fungal taxa associated with the analysed substrates.
format	article
author	Martina Vašutová Martin Jiroušek Michal Hájek
author_facet	Martina Vašutová Martin Jiroušek Michal Hájek
author_sort	Martina Vašutová
title	High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs
title_short	High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs
title_full	High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs
title_fullStr	High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs
title_full_unstemmed	High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs
title_sort	high fungal substrate specificity limits the utility of environmental dna to detect fungal diversity in bogs
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f98f2636f5794ce7b19be1cda2b27362
work_keys_str_mv	AT martinavasutova highfungalsubstratespecificitylimitstheutilityofenvironmentaldnatodetectfungaldiversityinbogs AT martinjirousek highfungalsubstratespecificitylimitstheutilityofenvironmentaldnatodetectfungaldiversityinbogs AT michalhajek highfungalsubstratespecificitylimitstheutilityofenvironmentaldnatodetectfungaldiversityinbogs
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High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs

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