The Transcriptome and Metabolome Reveal Stress Responses in Sulfur-Fumigated Cucumber (Cucumis sativus L.)
Sulfur (S) fumigation is a commonly used sterilization method in horticultural facilities against fungal diseases. S fumigation damaged cucumber leaves, although the response mechanism is unclear. This study analyzes the growth, transcriptome, and metabolomic profiles of young and mature leaves, ova...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:f9905cf75b46402caf02cf6abbd99af52021-11-12T17:14:19ZThe Transcriptome and Metabolome Reveal Stress Responses in Sulfur-Fumigated Cucumber (Cucumis sativus L.)1664-462X10.3389/fpls.2021.778956https://doaj.org/article/f9905cf75b46402caf02cf6abbd99af52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.778956/fullhttps://doaj.org/toc/1664-462XSulfur (S) fumigation is a commonly used sterilization method in horticultural facilities against fungal diseases. S fumigation damaged cucumber leaves, although the response mechanism is unclear. This study analyzes the growth, transcriptome, and metabolomic profiles of young and mature leaves, ovaries, and commercial cucumber fruits to decipher the mechanism of cucumber stress response under S fumigation. S fumigation significantly changed the photosynthetic efficiency and reactive oxygen species (ROS) in leaves, but not fruit development, fruit mass, and peel color. Transcriptome analysis indicated that S fumigation strongly regulated stress defense genes. The weighted gene co-expression network analysis revealed that S fumigation regulated ASPG1, AMC1 defense genes, LECRK3, and PERK1 protein kinase. The abscisic acid (ABA)-mediated model of regulation under S fumigation was constructed. Metabolome analysis showed that S fumigation significantly upregulated or downregulated the contents of amino acids, organic acids, sugars, glycosides, and lipids (VIP > 1 and P-value < 0.05). The opposite Pearson’s correlations of these differential metabolites implied that cucumber had different metabolic patterns in short-term and long-term S fumigation. Besides, the elevated levels of proline and triglyceride indicated that stress-responsive mechanisms existed in S-fumigated cucumber. Moreover, the comprehensive analysis indicated that S fumigation elevated secondary S-containing metabolites but decreased sulfate absorption and transportation in cucumber. Overall, our results provided a comprehensive assessment of S fumigation on cucumber, which laid the theoretical foundation for S fumigation in protected cultivation.Juan LiuJuan LiuYang GaoYang GaoFeifei GongFeifei GongFeifan HouFeifan HouZhipeng ZhangZhipeng ZhangXiaojing ChengXiaojing ChengWei DuWei DuLingling ZhangLingling ZhangJinyao WangJinyao WangJin XuJin XuGuoming XingGuoming XingXiuping KangXiuping KangSen LiSen LiFrontiers Media S.A.articlecucumbersulfur fumigationstress responsetranscriptomemetabolomePlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021) |
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| collection |
DOAJ |
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EN |
| topic |
cucumber sulfur fumigation stress response transcriptome metabolome Plant culture SB1-1110 |
| spellingShingle |
cucumber sulfur fumigation stress response transcriptome metabolome Plant culture SB1-1110 Juan Liu Juan Liu Yang Gao Yang Gao Feifei Gong Feifei Gong Feifan Hou Feifan Hou Zhipeng Zhang Zhipeng Zhang Xiaojing Cheng Xiaojing Cheng Wei Du Wei Du Lingling Zhang Lingling Zhang Jinyao Wang Jinyao Wang Jin Xu Jin Xu Guoming Xing Guoming Xing Xiuping Kang Xiuping Kang Sen Li Sen Li The Transcriptome and Metabolome Reveal Stress Responses in Sulfur-Fumigated Cucumber (Cucumis sativus L.) |
| description |
Sulfur (S) fumigation is a commonly used sterilization method in horticultural facilities against fungal diseases. S fumigation damaged cucumber leaves, although the response mechanism is unclear. This study analyzes the growth, transcriptome, and metabolomic profiles of young and mature leaves, ovaries, and commercial cucumber fruits to decipher the mechanism of cucumber stress response under S fumigation. S fumigation significantly changed the photosynthetic efficiency and reactive oxygen species (ROS) in leaves, but not fruit development, fruit mass, and peel color. Transcriptome analysis indicated that S fumigation strongly regulated stress defense genes. The weighted gene co-expression network analysis revealed that S fumigation regulated ASPG1, AMC1 defense genes, LECRK3, and PERK1 protein kinase. The abscisic acid (ABA)-mediated model of regulation under S fumigation was constructed. Metabolome analysis showed that S fumigation significantly upregulated or downregulated the contents of amino acids, organic acids, sugars, glycosides, and lipids (VIP > 1 and P-value < 0.05). The opposite Pearson’s correlations of these differential metabolites implied that cucumber had different metabolic patterns in short-term and long-term S fumigation. Besides, the elevated levels of proline and triglyceride indicated that stress-responsive mechanisms existed in S-fumigated cucumber. Moreover, the comprehensive analysis indicated that S fumigation elevated secondary S-containing metabolites but decreased sulfate absorption and transportation in cucumber. Overall, our results provided a comprehensive assessment of S fumigation on cucumber, which laid the theoretical foundation for S fumigation in protected cultivation. |
| format |
article |
| author |
Juan Liu Juan Liu Yang Gao Yang Gao Feifei Gong Feifei Gong Feifan Hou Feifan Hou Zhipeng Zhang Zhipeng Zhang Xiaojing Cheng Xiaojing Cheng Wei Du Wei Du Lingling Zhang Lingling Zhang Jinyao Wang Jinyao Wang Jin Xu Jin Xu Guoming Xing Guoming Xing Xiuping Kang Xiuping Kang Sen Li Sen Li |
| author_facet |
Juan Liu Juan Liu Yang Gao Yang Gao Feifei Gong Feifei Gong Feifan Hou Feifan Hou Zhipeng Zhang Zhipeng Zhang Xiaojing Cheng Xiaojing Cheng Wei Du Wei Du Lingling Zhang Lingling Zhang Jinyao Wang Jinyao Wang Jin Xu Jin Xu Guoming Xing Guoming Xing Xiuping Kang Xiuping Kang Sen Li Sen Li |
| author_sort |
Juan Liu |
| title |
The Transcriptome and Metabolome Reveal Stress Responses in Sulfur-Fumigated Cucumber (Cucumis sativus L.) |
| title_short |
The Transcriptome and Metabolome Reveal Stress Responses in Sulfur-Fumigated Cucumber (Cucumis sativus L.) |
| title_full |
The Transcriptome and Metabolome Reveal Stress Responses in Sulfur-Fumigated Cucumber (Cucumis sativus L.) |
| title_fullStr |
The Transcriptome and Metabolome Reveal Stress Responses in Sulfur-Fumigated Cucumber (Cucumis sativus L.) |
| title_full_unstemmed |
The Transcriptome and Metabolome Reveal Stress Responses in Sulfur-Fumigated Cucumber (Cucumis sativus L.) |
| title_sort |
transcriptome and metabolome reveal stress responses in sulfur-fumigated cucumber (cucumis sativus l.) |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/f9905cf75b46402caf02cf6abbd99af5 |
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