Candidate chemosensory genes identified from the greater wax moth, Galleria mellonella, through a transcriptomic analysis

Abstract The greater wax moth, Galleria mellonella Linnaeus (Lepidoptera: Galleriinae), is a ubiquitous pest of the honeybee, and poses a serious threat to the global honeybee industry. G. mellonella pheromone system is unusual compared to other lepidopterans and provides a unique olfactory model fo...

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Autores principales:	Hong-Xia Zhao, Wan-Yu Xiao, Cong-Hui Ji, Qin Ren, Xiao-Shan Xia, Xue-Feng Zhang, Wen-Zhong Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f990af2836fa463f917145ff593f9cf7
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spelling	oai:doaj.org-article:f990af2836fa463f917145ff593f9cf72021-12-02T15:09:15ZCandidate chemosensory genes identified from the greater wax moth, Galleria mellonella, through a transcriptomic analysis10.1038/s41598-019-46532-x2045-2322https://doaj.org/article/f990af2836fa463f917145ff593f9cf72019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46532-xhttps://doaj.org/toc/2045-2322Abstract The greater wax moth, Galleria mellonella Linnaeus (Lepidoptera: Galleriinae), is a ubiquitous pest of the honeybee, and poses a serious threat to the global honeybee industry. G. mellonella pheromone system is unusual compared to other lepidopterans and provides a unique olfactory model for pheromone perception. To better understand the olfactory mechanisms in G. mellonella, we conducted a transcriptomic analysis on the antennae of both male and female adults of G. mellonella using high-throughput sequencing and annotated gene families potentially involved in chemoreception. We annotated 46 unigenes coding for odorant receptors, 25 for ionotropic receptors, two for sensory neuron membrane proteins, 22 for odorant binding proteins and 20 for chemosensory proteins. Expressed primarily in antennae were all the 46 odorant receptor unigenes, nine of the 14 ionotropic receptor unigenes, and two of the 22 unigenes coding for odorant binding proteins, suggesting their putative roles in olfaction. The expression of some of the identified unigenes were sex-specific, suggesting that they may have important functions in the reproductive behavior of the insect. Identification of the candidate unigenes and initial analyses on their expression profiles should facilitate functional studies in the future on chemoreception mechanisms in this species and related lepidopteran moths.Hong-Xia ZhaoWan-Yu XiaoCong-Hui JiQin RenXiao-Shan XiaXue-Feng ZhangWen-Zhong HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hong-Xia Zhao Wan-Yu Xiao Cong-Hui Ji Qin Ren Xiao-Shan Xia Xue-Feng Zhang Wen-Zhong Huang Candidate chemosensory genes identified from the greater wax moth, Galleria mellonella, through a transcriptomic analysis
description	Abstract The greater wax moth, Galleria mellonella Linnaeus (Lepidoptera: Galleriinae), is a ubiquitous pest of the honeybee, and poses a serious threat to the global honeybee industry. G. mellonella pheromone system is unusual compared to other lepidopterans and provides a unique olfactory model for pheromone perception. To better understand the olfactory mechanisms in G. mellonella, we conducted a transcriptomic analysis on the antennae of both male and female adults of G. mellonella using high-throughput sequencing and annotated gene families potentially involved in chemoreception. We annotated 46 unigenes coding for odorant receptors, 25 for ionotropic receptors, two for sensory neuron membrane proteins, 22 for odorant binding proteins and 20 for chemosensory proteins. Expressed primarily in antennae were all the 46 odorant receptor unigenes, nine of the 14 ionotropic receptor unigenes, and two of the 22 unigenes coding for odorant binding proteins, suggesting their putative roles in olfaction. The expression of some of the identified unigenes were sex-specific, suggesting that they may have important functions in the reproductive behavior of the insect. Identification of the candidate unigenes and initial analyses on their expression profiles should facilitate functional studies in the future on chemoreception mechanisms in this species and related lepidopteran moths.
format	article
author	Hong-Xia Zhao Wan-Yu Xiao Cong-Hui Ji Qin Ren Xiao-Shan Xia Xue-Feng Zhang Wen-Zhong Huang
author_facet	Hong-Xia Zhao Wan-Yu Xiao Cong-Hui Ji Qin Ren Xiao-Shan Xia Xue-Feng Zhang Wen-Zhong Huang
author_sort	Hong-Xia Zhao
title	Candidate chemosensory genes identified from the greater wax moth, Galleria mellonella, through a transcriptomic analysis
title_short	Candidate chemosensory genes identified from the greater wax moth, Galleria mellonella, through a transcriptomic analysis
title_full	Candidate chemosensory genes identified from the greater wax moth, Galleria mellonella, through a transcriptomic analysis
title_fullStr	Candidate chemosensory genes identified from the greater wax moth, Galleria mellonella, through a transcriptomic analysis
title_full_unstemmed	Candidate chemosensory genes identified from the greater wax moth, Galleria mellonella, through a transcriptomic analysis
title_sort	candidate chemosensory genes identified from the greater wax moth, galleria mellonella, through a transcriptomic analysis
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/f990af2836fa463f917145ff593f9cf7
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Candidate chemosensory genes identified from the greater wax moth, Galleria mellonella, through a transcriptomic analysis

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