Alternative use of Bacillus subtilis spores: protection against environmental oxidative stress in human normal keratinocytes

Abstract Inorganic trivalent arsenic is a major environmental pollutant and exposure to human results in many pathologies, including keratosis and carcinoma. Here, we analyzed the effects of B. subtilis spores on human normal keratinocytes in the presence of sodium arsenite oxidative stress. Pre-tre...

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Autores principales:	Ganna Petruk, Giuliana Donadio, Mariamichela Lanzilli, Rachele Isticato, Daria Maria Monti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9957e547c864a2daca8201f67efb09e
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spelling	oai:doaj.org-article:f9957e547c864a2daca8201f67efb09e2021-12-02T15:08:15ZAlternative use of Bacillus subtilis spores: protection against environmental oxidative stress in human normal keratinocytes10.1038/s41598-018-20153-22045-2322https://doaj.org/article/f9957e547c864a2daca8201f67efb09e2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20153-2https://doaj.org/toc/2045-2322Abstract Inorganic trivalent arsenic is a major environmental pollutant and exposure to human results in many pathologies, including keratosis and carcinoma. Here, we analyzed the effects of B. subtilis spores on human normal keratinocytes in the presence of sodium arsenite oxidative stress. Pre-treatment of cells with spores before inducing oxidative stress was able to keep normal levels of intracellular ROS, GSH and lipid peroxidation, as well as to inhibit the activation of the MAPK cascade. Moreover, spores showed a positive effect on cell proliferation, probably due to their binding on the cell surface and the activation of intracellular catalases. We found that spores exert their protective effect by the nuclear translocation of Nrf-2, involved in the activation of stress response genes. This, in turn, resulted in a protective effect against sodium arsenite stress injury, as oxidative stress markers were reported to physiological levels when cells were stressed before incubating them with spores. Therefore, B. subtilis spores can be considered as a new agent to counteract oxidative stress on normal human keratinocytes.Ganna PetrukGiuliana DonadioMariamichela LanzilliRachele IsticatoDaria Maria MontiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ganna Petruk Giuliana Donadio Mariamichela Lanzilli Rachele Isticato Daria Maria Monti Alternative use of Bacillus subtilis spores: protection against environmental oxidative stress in human normal keratinocytes
description	Abstract Inorganic trivalent arsenic is a major environmental pollutant and exposure to human results in many pathologies, including keratosis and carcinoma. Here, we analyzed the effects of B. subtilis spores on human normal keratinocytes in the presence of sodium arsenite oxidative stress. Pre-treatment of cells with spores before inducing oxidative stress was able to keep normal levels of intracellular ROS, GSH and lipid peroxidation, as well as to inhibit the activation of the MAPK cascade. Moreover, spores showed a positive effect on cell proliferation, probably due to their binding on the cell surface and the activation of intracellular catalases. We found that spores exert their protective effect by the nuclear translocation of Nrf-2, involved in the activation of stress response genes. This, in turn, resulted in a protective effect against sodium arsenite stress injury, as oxidative stress markers were reported to physiological levels when cells were stressed before incubating them with spores. Therefore, B. subtilis spores can be considered as a new agent to counteract oxidative stress on normal human keratinocytes.
format	article
author	Ganna Petruk Giuliana Donadio Mariamichela Lanzilli Rachele Isticato Daria Maria Monti
author_facet	Ganna Petruk Giuliana Donadio Mariamichela Lanzilli Rachele Isticato Daria Maria Monti
author_sort	Ganna Petruk
title	Alternative use of Bacillus subtilis spores: protection against environmental oxidative stress in human normal keratinocytes
title_short	Alternative use of Bacillus subtilis spores: protection against environmental oxidative stress in human normal keratinocytes
title_full	Alternative use of Bacillus subtilis spores: protection against environmental oxidative stress in human normal keratinocytes
title_fullStr	Alternative use of Bacillus subtilis spores: protection against environmental oxidative stress in human normal keratinocytes
title_full_unstemmed	Alternative use of Bacillus subtilis spores: protection against environmental oxidative stress in human normal keratinocytes
title_sort	alternative use of bacillus subtilis spores: protection against environmental oxidative stress in human normal keratinocytes
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f9957e547c864a2daca8201f67efb09e
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Alternative use of Bacillus subtilis spores: protection against environmental oxidative stress in human normal keratinocytes

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