The relationship of smoking to cg05575921 methylation in blood and saliva DNA samples from several studies

Abstract Numerous studies have shown that cg05575921 methylation decreases in response to smoking. However, secondary to methodological issues, the magnitude and dose dependency of that response is as of yet unclear. This lack of certainty is a barrier to the use of DNA methylation clinically to ass...

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Autores principales:	Kelsey Dawes, Allan Andersen, Rachel Reimer, James A. Mills, Eric Hoffman, Jeffrey D. Long, Shelly Miller, Robert Philibert
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9a2397189974920a5250144371a2f3c
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spelling	oai:doaj.org-article:f9a2397189974920a5250144371a2f3c2021-11-08T10:53:54ZThe relationship of smoking to cg05575921 methylation in blood and saliva DNA samples from several studies10.1038/s41598-021-01088-72045-2322https://doaj.org/article/f9a2397189974920a5250144371a2f3c2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01088-7https://doaj.org/toc/2045-2322Abstract Numerous studies have shown that cg05575921 methylation decreases in response to smoking. However, secondary to methodological issues, the magnitude and dose dependency of that response is as of yet unclear. This lack of certainty is a barrier to the use of DNA methylation clinically to assess and monitor smoking status. To better define this relationship, we conducted a joint analysis of methylation sensitive PCR digital (MSdPCR) assessments of cg05575921 methylation in whole blood and/or saliva DNA to smoking using samples from 421 smokers and 423 biochemically confirmed non-smokers from 4 previously published studies. We found that cg05575921 methylation manifested a curvilinear dose dependent decrease in response to increasing cigarette consumption. In whole blood DNA, the Receiver Operating Characteristic (ROC) Area Under the Curve (AUC) of cg05575921 methylation for predicting daily smoking status was 0.98. In saliva DNA, the gross AUC was 0.91 with correction for cellular heterogeneity improving the AUC to 0.94. Methylation status was significantly associated with the Fagerstrom Test for Nicotine Dependence score, but with significant sampling heterogeneity. We conclude that MSdPCR assessments of cg05575921 methylation are a potentially powerful, clinically implementable tool for the assessment and management of smoking.Kelsey DawesAllan AndersenRachel ReimerJames A. MillsEric HoffmanJeffrey D. LongShelly MillerRobert PhilibertNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kelsey Dawes Allan Andersen Rachel Reimer James A. Mills Eric Hoffman Jeffrey D. Long Shelly Miller Robert Philibert The relationship of smoking to cg05575921 methylation in blood and saliva DNA samples from several studies
description	Abstract Numerous studies have shown that cg05575921 methylation decreases in response to smoking. However, secondary to methodological issues, the magnitude and dose dependency of that response is as of yet unclear. This lack of certainty is a barrier to the use of DNA methylation clinically to assess and monitor smoking status. To better define this relationship, we conducted a joint analysis of methylation sensitive PCR digital (MSdPCR) assessments of cg05575921 methylation in whole blood and/or saliva DNA to smoking using samples from 421 smokers and 423 biochemically confirmed non-smokers from 4 previously published studies. We found that cg05575921 methylation manifested a curvilinear dose dependent decrease in response to increasing cigarette consumption. In whole blood DNA, the Receiver Operating Characteristic (ROC) Area Under the Curve (AUC) of cg05575921 methylation for predicting daily smoking status was 0.98. In saliva DNA, the gross AUC was 0.91 with correction for cellular heterogeneity improving the AUC to 0.94. Methylation status was significantly associated with the Fagerstrom Test for Nicotine Dependence score, but with significant sampling heterogeneity. We conclude that MSdPCR assessments of cg05575921 methylation are a potentially powerful, clinically implementable tool for the assessment and management of smoking.
format	article
author	Kelsey Dawes Allan Andersen Rachel Reimer James A. Mills Eric Hoffman Jeffrey D. Long Shelly Miller Robert Philibert
author_facet	Kelsey Dawes Allan Andersen Rachel Reimer James A. Mills Eric Hoffman Jeffrey D. Long Shelly Miller Robert Philibert
author_sort	Kelsey Dawes
title	The relationship of smoking to cg05575921 methylation in blood and saliva DNA samples from several studies
title_short	The relationship of smoking to cg05575921 methylation in blood and saliva DNA samples from several studies
title_full	The relationship of smoking to cg05575921 methylation in blood and saliva DNA samples from several studies
title_fullStr	The relationship of smoking to cg05575921 methylation in blood and saliva DNA samples from several studies
title_full_unstemmed	The relationship of smoking to cg05575921 methylation in blood and saliva DNA samples from several studies
title_sort	relationship of smoking to cg05575921 methylation in blood and saliva dna samples from several studies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f9a2397189974920a5250144371a2f3c
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The relationship of smoking to cg05575921 methylation in blood and saliva DNA samples from several studies

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