Smoothness correction for better SOFI imaging

Abstract Sub-diffraction or super-resolution fluorescence imaging allows the visualization of the cellular morphology and interactions at the nanoscale. Statistical analysis methods such as super-resolution optical fluctuation imaging (SOFI) obtain an improved spatial resolution by analyzing fluorop...

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Autores principales:	Siewert Hugelier, Wim Vandenberg, Tomáš Lukeš, Kristin S. Grußmayer, Paul H. C. Eilers, Peter Dedecker, Cyril Ruckebusch
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9a2e049d318479aae5d45359e047cf9
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id	oai:doaj.org-article:f9a2e049d318479aae5d45359e047cf9
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spelling	oai:doaj.org-article:f9a2e049d318479aae5d45359e047cf92021-12-02T14:37:29ZSmoothness correction for better SOFI imaging10.1038/s41598-021-87164-42045-2322https://doaj.org/article/f9a2e049d318479aae5d45359e047cf92021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87164-4https://doaj.org/toc/2045-2322Abstract Sub-diffraction or super-resolution fluorescence imaging allows the visualization of the cellular morphology and interactions at the nanoscale. Statistical analysis methods such as super-resolution optical fluctuation imaging (SOFI) obtain an improved spatial resolution by analyzing fluorophore blinking but can be perturbed by the presence of non-stationary processes such as photodestruction or fluctuations in the illumination. In this work, we propose to use Whittaker smoothing to remove these smooth signal trends and retain only the information associated to independent blinking of the emitters, thus enhancing the SOFI signals. We find that our method works well to correct photodestruction, especially when it occurs quickly. The resulting images show a much higher contrast, strongly suppressed background and a more detailed visualization of cellular structures. Our method is parameter-free and computationally efficient, and can be readily applied on both two-dimensional and three-dimensional data.Siewert HugelierWim VandenbergTomáš LukešKristin S. GrußmayerPaul H. C. EilersPeter DedeckerCyril RuckebuschNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Siewert Hugelier Wim Vandenberg Tomáš Lukeš Kristin S. Grußmayer Paul H. C. Eilers Peter Dedecker Cyril Ruckebusch Smoothness correction for better SOFI imaging
description	Abstract Sub-diffraction or super-resolution fluorescence imaging allows the visualization of the cellular morphology and interactions at the nanoscale. Statistical analysis methods such as super-resolution optical fluctuation imaging (SOFI) obtain an improved spatial resolution by analyzing fluorophore blinking but can be perturbed by the presence of non-stationary processes such as photodestruction or fluctuations in the illumination. In this work, we propose to use Whittaker smoothing to remove these smooth signal trends and retain only the information associated to independent blinking of the emitters, thus enhancing the SOFI signals. We find that our method works well to correct photodestruction, especially when it occurs quickly. The resulting images show a much higher contrast, strongly suppressed background and a more detailed visualization of cellular structures. Our method is parameter-free and computationally efficient, and can be readily applied on both two-dimensional and three-dimensional data.
format	article
author	Siewert Hugelier Wim Vandenberg Tomáš Lukeš Kristin S. Grußmayer Paul H. C. Eilers Peter Dedecker Cyril Ruckebusch
author_facet	Siewert Hugelier Wim Vandenberg Tomáš Lukeš Kristin S. Grußmayer Paul H. C. Eilers Peter Dedecker Cyril Ruckebusch
author_sort	Siewert Hugelier
title	Smoothness correction for better SOFI imaging
title_short	Smoothness correction for better SOFI imaging
title_full	Smoothness correction for better SOFI imaging
title_fullStr	Smoothness correction for better SOFI imaging
title_full_unstemmed	Smoothness correction for better SOFI imaging
title_sort	smoothness correction for better sofi imaging
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f9a2e049d318479aae5d45359e047cf9
work_keys_str_mv	AT siewerthugelier smoothnesscorrectionforbettersofiimaging AT wimvandenberg smoothnesscorrectionforbettersofiimaging AT tomaslukes smoothnesscorrectionforbettersofiimaging AT kristinsgrußmayer smoothnesscorrectionforbettersofiimaging AT paulhceilers smoothnesscorrectionforbettersofiimaging AT peterdedecker smoothnesscorrectionforbettersofiimaging AT cyrilruckebusch smoothnesscorrectionforbettersofiimaging
_version_	1718391047554334720

Smoothness correction for better SOFI imaging

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