Platelet function and bleeding at different phases of childhood immune thrombocytopenia

Abstract Immune thrombocytopenia (ITP) is believed to be associated with platelet function defects. However, their mechanisms are poorly understood, in particular with regard to differences between ITP phases, patient age, and therapy. We investigated platelet function and bleeding in children with...

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Autores principales:	Anastasia A. Ignatova, Elena V. Suntsova, Alexey V. Pshonkin, Alexey A. Martyanov, Evgeniya A. Ponomarenko, Dmitry M. Polokhov, Daria V. Fedorova, Kirill A. Voronin, Natalia N. Kotskaya, Natalia M. Trubina, Marina V. Krasilnikova, Selima Sh. Uzueva, Irina V. Serkova, Galina S. Ovsyannikova, Ksenia I. Romanova, Lili A. Hachatryan, Irina I. Kalinina, Viktor E. Matveev, Maya N. Korsantiya, Natalia S. Smetanina, Dmitry A. Evseev, Maria N. Sadovskaya, Kristina S. Antonova, Anna L. Khoreva, Pavel A. Zharkov, Anna Shcherbina, Anastasia N. Sveshnikova, Aleksey A. Maschan, Galina A. Novichkova, Mikhail A. Panteleev
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9a40e6bd1604587948d4d6969bfc9ed
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spelling	oai:doaj.org-article:f9a40e6bd1604587948d4d6969bfc9ed2021-12-02T13:41:34ZPlatelet function and bleeding at different phases of childhood immune thrombocytopenia10.1038/s41598-021-88900-62045-2322https://doaj.org/article/f9a40e6bd1604587948d4d6969bfc9ed2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88900-6https://doaj.org/toc/2045-2322Abstract Immune thrombocytopenia (ITP) is believed to be associated with platelet function defects. However, their mechanisms are poorly understood, in particular with regard to differences between ITP phases, patient age, and therapy. We investigated platelet function and bleeding in children with either persistent or chronic ITP, with or without romiplostim therapy. The study included 151 children with ITP, of whom 56 had disease duration less than 12 months (grouped together as acute/persistent) and 95 were chronic. Samples of 57 healthy children were used as controls, while 5 patients with leukemia, 5 with aplastic anemia, 4 with MYH9-associated thrombocytopenia, and 7 with Wiskott-Aldrich syndrome were used as non-ITP thrombocytopenia controls. Whole blood flow cytometry revealed that platelets in both acute/persistent and chronic ITP were increased in size compared with healthy donors. They were also pre-activated as assessed by PAC1, CD62p, cytosolic calcium, and procoagulant platelet levels. This pattern was not observed in other childhood thrombocytopenias. Pre-activation by CD62p was higher in the bleeding group in the chronic ITP cohort only. Romiplostim treatment decreased size and pre-activation of the patient platelets, but not calcium. Our data suggest that increased size, pre-activation, and cytosolic calcium are common for all ITP platelets, but their association with bleeding could depend on the disease phase.Anastasia A. IgnatovaElena V. SuntsovaAlexey V. PshonkinAlexey A. MartyanovEvgeniya A. PonomarenkoDmitry M. PolokhovDaria V. FedorovaKirill A. VoroninNatalia N. KotskayaNatalia M. TrubinaMarina V. KrasilnikovaSelima Sh. UzuevaIrina V. SerkovaGalina S. OvsyannikovaKsenia I. RomanovaLili A. HachatryanIrina I. KalininaViktor E. MatveevMaya N. KorsantiyaNatalia S. SmetaninaDmitry A. EvseevMaria N. SadovskayaKristina S. AntonovaAnna L. KhorevaPavel A. ZharkovAnna ShcherbinaAnastasia N. SveshnikovaAleksey A. MaschanGalina A. NovichkovaMikhail A. PanteleevNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anastasia A. Ignatova Elena V. Suntsova Alexey V. Pshonkin Alexey A. Martyanov Evgeniya A. Ponomarenko Dmitry M. Polokhov Daria V. Fedorova Kirill A. Voronin Natalia N. Kotskaya Natalia M. Trubina Marina V. Krasilnikova Selima Sh. Uzueva Irina V. Serkova Galina S. Ovsyannikova Ksenia I. Romanova Lili A. Hachatryan Irina I. Kalinina Viktor E. Matveev Maya N. Korsantiya Natalia S. Smetanina Dmitry A. Evseev Maria N. Sadovskaya Kristina S. Antonova Anna L. Khoreva Pavel A. Zharkov Anna Shcherbina Anastasia N. Sveshnikova Aleksey A. Maschan Galina A. Novichkova Mikhail A. Panteleev Platelet function and bleeding at different phases of childhood immune thrombocytopenia
description	Abstract Immune thrombocytopenia (ITP) is believed to be associated with platelet function defects. However, their mechanisms are poorly understood, in particular with regard to differences between ITP phases, patient age, and therapy. We investigated platelet function and bleeding in children with either persistent or chronic ITP, with or without romiplostim therapy. The study included 151 children with ITP, of whom 56 had disease duration less than 12 months (grouped together as acute/persistent) and 95 were chronic. Samples of 57 healthy children were used as controls, while 5 patients with leukemia, 5 with aplastic anemia, 4 with MYH9-associated thrombocytopenia, and 7 with Wiskott-Aldrich syndrome were used as non-ITP thrombocytopenia controls. Whole blood flow cytometry revealed that platelets in both acute/persistent and chronic ITP were increased in size compared with healthy donors. They were also pre-activated as assessed by PAC1, CD62p, cytosolic calcium, and procoagulant platelet levels. This pattern was not observed in other childhood thrombocytopenias. Pre-activation by CD62p was higher in the bleeding group in the chronic ITP cohort only. Romiplostim treatment decreased size and pre-activation of the patient platelets, but not calcium. Our data suggest that increased size, pre-activation, and cytosolic calcium are common for all ITP platelets, but their association with bleeding could depend on the disease phase.
format	article
author	Anastasia A. Ignatova Elena V. Suntsova Alexey V. Pshonkin Alexey A. Martyanov Evgeniya A. Ponomarenko Dmitry M. Polokhov Daria V. Fedorova Kirill A. Voronin Natalia N. Kotskaya Natalia M. Trubina Marina V. Krasilnikova Selima Sh. Uzueva Irina V. Serkova Galina S. Ovsyannikova Ksenia I. Romanova Lili A. Hachatryan Irina I. Kalinina Viktor E. Matveev Maya N. Korsantiya Natalia S. Smetanina Dmitry A. Evseev Maria N. Sadovskaya Kristina S. Antonova Anna L. Khoreva Pavel A. Zharkov Anna Shcherbina Anastasia N. Sveshnikova Aleksey A. Maschan Galina A. Novichkova Mikhail A. Panteleev
author_facet	Anastasia A. Ignatova Elena V. Suntsova Alexey V. Pshonkin Alexey A. Martyanov Evgeniya A. Ponomarenko Dmitry M. Polokhov Daria V. Fedorova Kirill A. Voronin Natalia N. Kotskaya Natalia M. Trubina Marina V. Krasilnikova Selima Sh. Uzueva Irina V. Serkova Galina S. Ovsyannikova Ksenia I. Romanova Lili A. Hachatryan Irina I. Kalinina Viktor E. Matveev Maya N. Korsantiya Natalia S. Smetanina Dmitry A. Evseev Maria N. Sadovskaya Kristina S. Antonova Anna L. Khoreva Pavel A. Zharkov Anna Shcherbina Anastasia N. Sveshnikova Aleksey A. Maschan Galina A. Novichkova Mikhail A. Panteleev
author_sort	Anastasia A. Ignatova
title	Platelet function and bleeding at different phases of childhood immune thrombocytopenia
title_short	Platelet function and bleeding at different phases of childhood immune thrombocytopenia
title_full	Platelet function and bleeding at different phases of childhood immune thrombocytopenia
title_fullStr	Platelet function and bleeding at different phases of childhood immune thrombocytopenia
title_full_unstemmed	Platelet function and bleeding at different phases of childhood immune thrombocytopenia
title_sort	platelet function and bleeding at different phases of childhood immune thrombocytopenia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f9a40e6bd1604587948d4d6969bfc9ed
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Platelet function and bleeding at different phases of childhood immune thrombocytopenia

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