The ability of streptomycin-loaded chitosan-coated magnetic nanocomposites to possess antimicrobial and antituberculosis activities

Mohamed Ezzat El Zowalaty,1,2 Samer Hassan Hussein Al Ali,3,4 Mohamed I Husseiny,2,5 Benjamin M Geilich,6,7 Thomas J Webster,7,8 Mohd Zobir Hussein9 1Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia; 2Department of Mic...

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Autores principales: El Zowalaty ME, Hussein Al Ali SH, Husseiny MI, Geilich BM, Webster TJ, Hussein MZ
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Publicado: Dove Medical Press 2015
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spelling oai:doaj.org-article:f9a6d0bcf87b48adb42d7dc46d2f43d62021-12-02T07:37:00ZThe ability of streptomycin-loaded chitosan-coated magnetic nanocomposites to possess antimicrobial and antituberculosis activities1178-2013https://doaj.org/article/f9a6d0bcf87b48adb42d7dc46d2f43d62015-04-01T00:00:00Zhttp://www.dovepress.com/the-ability-of-streptomycin-loaded-chitosan-coated-magnetic-nanocompos-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mohamed Ezzat El Zowalaty,1,2 Samer Hassan Hussein Al Ali,3,4 Mohamed I Husseiny,2,5 Benjamin M Geilich,6,7 Thomas J Webster,7,8 Mohd Zobir Hussein9 1Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia; 2Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt; 3Laboratory of Molecular Biomedicine, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia; 4Faculty of Pharmacy, Isra University, Amman, Jordan; 5Beckman Research Institute of City of Hope, Duarte, CA, USA; 6Department of Bioengineering, 7Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 8Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; 9Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology; Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia Abstract: Magnetic nanoparticles (MNPs) were synthesized by the coprecipitation of Fe2+ and Fe3+ iron salts in alkali media. MNPs were coated by chitosan (CS) to produce CS-MNPs. Streptomycin (Strep) was loaded onto the surface of CS-MNPs to form a Strep-CS-MNP nanocomposite. MNPs, CS-MNPs, and the nanocomposites were subsequently characterized using X-ray diffraction and were evaluated for their antibacterial activity. The antimicrobial activity of the as-synthesized nanoparticles was evaluated using different Gram-positive and Gram-negative bacteria, as well as Mycobacterium tuberculosis. For the first time, it was found that the nanoparticles showed antimicrobial activities against the tested microorganisms (albeit with a more pronounced effect against Gram-negative than Gram-positive bacteria), and thus, should be further studied as a novel nano-antibiotic for numerous antimicrobial and antituberculosis applications. Moreover, since these nanoparticle bacteria fighters are magnetic, one can easily envision magnetic field direction of these nanoparticles to fight unwanted microorganism presence on demand. Due to the ability of magnetic nanoparticles to increase the sensitivity of imaging modalities (such as magnetic resonance imaging), these novel nanoparticles can also be used to diagnose the presence of such microorganisms. In summary, although requiring further investigation, this study introduces for the first time a new type of magnetic nanoparticle with microorganism theranostic properties as a potential tool to both diagnose and treat diverse microbial and tuberculosis infections. Keywords: iron oxide nanoparticles, chitosan, streptomycin, Mycobacterium tuberculosis, antimicrobial activityEl Zowalaty MEHussein Al Ali SHHusseiny MIGeilich BMWebster TJHussein MZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 3269-3274 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
El Zowalaty ME
Hussein Al Ali SH
Husseiny MI
Geilich BM
Webster TJ
Hussein MZ
The ability of streptomycin-loaded chitosan-coated magnetic nanocomposites to possess antimicrobial and antituberculosis activities
description Mohamed Ezzat El Zowalaty,1,2 Samer Hassan Hussein Al Ali,3,4 Mohamed I Husseiny,2,5 Benjamin M Geilich,6,7 Thomas J Webster,7,8 Mohd Zobir Hussein9 1Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia; 2Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt; 3Laboratory of Molecular Biomedicine, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia; 4Faculty of Pharmacy, Isra University, Amman, Jordan; 5Beckman Research Institute of City of Hope, Duarte, CA, USA; 6Department of Bioengineering, 7Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 8Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; 9Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology; Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia Abstract: Magnetic nanoparticles (MNPs) were synthesized by the coprecipitation of Fe2+ and Fe3+ iron salts in alkali media. MNPs were coated by chitosan (CS) to produce CS-MNPs. Streptomycin (Strep) was loaded onto the surface of CS-MNPs to form a Strep-CS-MNP nanocomposite. MNPs, CS-MNPs, and the nanocomposites were subsequently characterized using X-ray diffraction and were evaluated for their antibacterial activity. The antimicrobial activity of the as-synthesized nanoparticles was evaluated using different Gram-positive and Gram-negative bacteria, as well as Mycobacterium tuberculosis. For the first time, it was found that the nanoparticles showed antimicrobial activities against the tested microorganisms (albeit with a more pronounced effect against Gram-negative than Gram-positive bacteria), and thus, should be further studied as a novel nano-antibiotic for numerous antimicrobial and antituberculosis applications. Moreover, since these nanoparticle bacteria fighters are magnetic, one can easily envision magnetic field direction of these nanoparticles to fight unwanted microorganism presence on demand. Due to the ability of magnetic nanoparticles to increase the sensitivity of imaging modalities (such as magnetic resonance imaging), these novel nanoparticles can also be used to diagnose the presence of such microorganisms. In summary, although requiring further investigation, this study introduces for the first time a new type of magnetic nanoparticle with microorganism theranostic properties as a potential tool to both diagnose and treat diverse microbial and tuberculosis infections. Keywords: iron oxide nanoparticles, chitosan, streptomycin, Mycobacterium tuberculosis, antimicrobial activity
format article
author El Zowalaty ME
Hussein Al Ali SH
Husseiny MI
Geilich BM
Webster TJ
Hussein MZ
author_facet El Zowalaty ME
Hussein Al Ali SH
Husseiny MI
Geilich BM
Webster TJ
Hussein MZ
author_sort El Zowalaty ME
title The ability of streptomycin-loaded chitosan-coated magnetic nanocomposites to possess antimicrobial and antituberculosis activities
title_short The ability of streptomycin-loaded chitosan-coated magnetic nanocomposites to possess antimicrobial and antituberculosis activities
title_full The ability of streptomycin-loaded chitosan-coated magnetic nanocomposites to possess antimicrobial and antituberculosis activities
title_fullStr The ability of streptomycin-loaded chitosan-coated magnetic nanocomposites to possess antimicrobial and antituberculosis activities
title_full_unstemmed The ability of streptomycin-loaded chitosan-coated magnetic nanocomposites to possess antimicrobial and antituberculosis activities
title_sort ability of streptomycin-loaded chitosan-coated magnetic nanocomposites to possess antimicrobial and antituberculosis activities
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/f9a6d0bcf87b48adb42d7dc46d2f43d6
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