Evaluation of the genotoxicity of cellulose nanofibers

Renata de Lima,1 Leandro Oliveira Feitosa,1 Cintia Rodrigues Maruyama,1 Mariana Abreu Barga,1 Patr&iacute;cia Cristina Yamawaki,1 Isolda Jesus Vieira,1 Eliangela M Teixeira,2 Ana Carolina Corr&ecirc;a,2 Luiz Henrique Caparelli Mattoso,2 Leonardo Fernandes Fraceto31Department of Biote...

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Autores principales:	de Lima R, Feitosa LO, Maruyama CR, Barga MA, Yamawaki PC, Vieira IJ, Teixeira EM, Corr&ecirc;a AC, Mattoso LH, Fraceto LF
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f9a7e5ef71e5484a9e0e34b6a439ba56
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spelling	oai:doaj.org-article:f9a7e5ef71e5484a9e0e34b6a439ba562021-12-02T08:07:36ZEvaluation of the genotoxicity of cellulose nanofibers1176-91141178-2013https://doaj.org/article/f9a7e5ef71e5484a9e0e34b6a439ba562012-07-01T00:00:00Zhttp://www.dovepress.com/evaluation-of-the-genotoxicity-of-cellulose-nanofibers-a10356https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Renata de Lima,1 Leandro Oliveira Feitosa,1 Cintia Rodrigues Maruyama,1 Mariana Abreu Barga,1 Patr&iacute;cia Cristina Yamawaki,1 Isolda Jesus Vieira,1 Eliangela M Teixeira,2 Ana Carolina Corr&ecirc;a,2 Luiz Henrique Caparelli Mattoso,2 Leonardo Fernandes Fraceto31Department of Biotechnology, University of Sorocaba, Sorocaba, 2Embrapa Instrumentation (CNPDIA), National Nanotechnology Laboratory for Agriculture (LNNA), S&atilde;o Carlos, 3Department of Environmental Engineering, State University of S&atilde;o Paulo (UNESP), Sorocaba, SP, BrazilBackground: Agricultural products and by products provide the primary materials for a variety of technological applications in diverse industrial sectors. Agro-industrial wastes, such as cotton and curaua fibers, are used to prepare nanofibers for use in thermoplastic films, where they are combined with polymeric matrices, and in biomedical applications such as tissue engineering, amongst other applications. The development of products containing nanofibers offers a promising alternative for the use of agricultural products, adding value to the chains of production. However, the emergence of new nanotechnological products demands that their risks to human health and the environment be evaluated. This has resulted in the creation of the new area of nanotoxicology, which addresses the toxicological aspects of these materials.Purpose and methods: Contributing to these developments, the present work involved a genotoxicological study of different nanofibers, employing chromosomal aberration and comet assays, as well as cytogenetic and molecular analyses, to obtain preliminary information concerning nanofiber safety. The methodology consisted of exposure of Allium cepa roots, and animal cell cultures (lymphocytes and fibroblasts), to different types of nanofibers. Negative controls, without nanofibers present in the medium, were used for comparison.Results: The nanofibers induced different responses according to the cell type used. In plant cells, the most genotoxic nanofibers were those derived from green, white, and brown cotton, and curaua, while genotoxicity in animal cells was observed using nanofibers from brown cotton and curaua. An important finding was that ruby cotton nanofibers did not cause any significant DNA breaks in the cell types employed.Conclusion: This work demonstrates the feasibility of determining the genotoxic potential of nanofibers derived from plant cellulose to obtain information vital both for the future usage of these materials in agribusiness and for an understanding of their environmental impacts.Keywords: cotton, curaua, nanotoxicology, environmental nanotechnologyde Lima RFeitosa LOMaruyama CRBarga MAYamawaki PCVieira IJTeixeira EMCorr&ecirc;a ACMattoso LHFraceto LFDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3555-3565 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 de Lima R Feitosa LO Maruyama CR Barga MA Yamawaki PC Vieira IJ Teixeira EM Corr&ecirc;a AC Mattoso LH Fraceto LF Evaluation of the genotoxicity of cellulose nanofibers
description	Renata de Lima,1 Leandro Oliveira Feitosa,1 Cintia Rodrigues Maruyama,1 Mariana Abreu Barga,1 Patr&iacute;cia Cristina Yamawaki,1 Isolda Jesus Vieira,1 Eliangela M Teixeira,2 Ana Carolina Corr&ecirc;a,2 Luiz Henrique Caparelli Mattoso,2 Leonardo Fernandes Fraceto31Department of Biotechnology, University of Sorocaba, Sorocaba, 2Embrapa Instrumentation (CNPDIA), National Nanotechnology Laboratory for Agriculture (LNNA), S&atilde;o Carlos, 3Department of Environmental Engineering, State University of S&atilde;o Paulo (UNESP), Sorocaba, SP, BrazilBackground: Agricultural products and by products provide the primary materials for a variety of technological applications in diverse industrial sectors. Agro-industrial wastes, such as cotton and curaua fibers, are used to prepare nanofibers for use in thermoplastic films, where they are combined with polymeric matrices, and in biomedical applications such as tissue engineering, amongst other applications. The development of products containing nanofibers offers a promising alternative for the use of agricultural products, adding value to the chains of production. However, the emergence of new nanotechnological products demands that their risks to human health and the environment be evaluated. This has resulted in the creation of the new area of nanotoxicology, which addresses the toxicological aspects of these materials.Purpose and methods: Contributing to these developments, the present work involved a genotoxicological study of different nanofibers, employing chromosomal aberration and comet assays, as well as cytogenetic and molecular analyses, to obtain preliminary information concerning nanofiber safety. The methodology consisted of exposure of Allium cepa roots, and animal cell cultures (lymphocytes and fibroblasts), to different types of nanofibers. Negative controls, without nanofibers present in the medium, were used for comparison.Results: The nanofibers induced different responses according to the cell type used. In plant cells, the most genotoxic nanofibers were those derived from green, white, and brown cotton, and curaua, while genotoxicity in animal cells was observed using nanofibers from brown cotton and curaua. An important finding was that ruby cotton nanofibers did not cause any significant DNA breaks in the cell types employed.Conclusion: This work demonstrates the feasibility of determining the genotoxic potential of nanofibers derived from plant cellulose to obtain information vital both for the future usage of these materials in agribusiness and for an understanding of their environmental impacts.Keywords: cotton, curaua, nanotoxicology, environmental nanotechnology
format	article
author	de Lima R Feitosa LO Maruyama CR Barga MA Yamawaki PC Vieira IJ Teixeira EM Corr&ecirc;a AC Mattoso LH Fraceto LF
author_facet	de Lima R Feitosa LO Maruyama CR Barga MA Yamawaki PC Vieira IJ Teixeira EM Corr&ecirc;a AC Mattoso LH Fraceto LF
author_sort	de Lima R
title	Evaluation of the genotoxicity of cellulose nanofibers
title_short	Evaluation of the genotoxicity of cellulose nanofibers
title_full	Evaluation of the genotoxicity of cellulose nanofibers
title_fullStr	Evaluation of the genotoxicity of cellulose nanofibers
title_full_unstemmed	Evaluation of the genotoxicity of cellulose nanofibers
title_sort	evaluation of the genotoxicity of cellulose nanofibers
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/f9a7e5ef71e5484a9e0e34b6a439ba56
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Evaluation of the genotoxicity of cellulose nanofibers

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