PLAG1 deficiency impairs spermatogenesis and sperm motility in mice

Abstract Deficiency in pleomorphic adenoma gene 1 (PLAG1) leads to reduced fertility in male mice, but the mechanism by which PLAG1 contributes to reproduction is unknown. To investigate the involvement of PLAG1 in testicular function, we determined (i) the spatial distribution of PLAG1 in the testi...

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Autores principales:	Almas R. Juma, Sylvia V. H. Grommen, Moira K. O’Bryan, Anne E. O’Connor, D. Jo Merriner, Nathan E. Hall, Stephen R. Doyle, Pauliina E. Damdimopoulou, Daniel Barriga, Adam H. Hart, Wim J. M. Van de Ven, Bert De Groef
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9a9c5dca672498dbf31f37666823195
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spelling	oai:doaj.org-article:f9a9c5dca672498dbf31f376668231952021-12-02T15:06:00ZPLAG1 deficiency impairs spermatogenesis and sperm motility in mice10.1038/s41598-017-05676-42045-2322https://doaj.org/article/f9a9c5dca672498dbf31f376668231952017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05676-4https://doaj.org/toc/2045-2322Abstract Deficiency in pleomorphic adenoma gene 1 (PLAG1) leads to reduced fertility in male mice, but the mechanism by which PLAG1 contributes to reproduction is unknown. To investigate the involvement of PLAG1 in testicular function, we determined (i) the spatial distribution of PLAG1 in the testis using X-gal staining; (ii) transcriptomic consequences of PLAG1 deficiency in knock-out and heterozygous mice compared to wild-type mice using RNA-seq; and (iii) morphological and functional consequences of PLAG1 deficiency by determining testicular histology, daily sperm production and sperm motility in knock-out and wild-type mice. PLAG1 was sparsely expressed in germ cells and in Sertoli cells. Genes known to be involved in spermatogenesis were downregulated in the testes of knock-out mice, as well as Hsd17b3, which encodes a key enzyme in androgen biosynthesis. In the absence of Plag1, a number of genes involved in immune processes and epididymis-specific genes were upregulated in the testes. Finally, loss of PLAG1 resulted in significantly lowered daily sperm production, in reduced sperm motility, and in several animals, in sloughing of the germinal epithelium. Our results demonstrate that the subfertility seen in male PLAG1-deficient mice is, at least in part, the result of significantly reduced sperm output and sperm motility.Almas R. JumaSylvia V. H. GrommenMoira K. O’BryanAnne E. O’ConnorD. Jo MerrinerNathan E. HallStephen R. DoylePauliina E. DamdimopoulouDaniel BarrigaAdam H. HartWim J. M. Van de VenBert De GroefNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Almas R. Juma Sylvia V. H. Grommen Moira K. O’Bryan Anne E. O’Connor D. Jo Merriner Nathan E. Hall Stephen R. Doyle Pauliina E. Damdimopoulou Daniel Barriga Adam H. Hart Wim J. M. Van de Ven Bert De Groef PLAG1 deficiency impairs spermatogenesis and sperm motility in mice
description	Abstract Deficiency in pleomorphic adenoma gene 1 (PLAG1) leads to reduced fertility in male mice, but the mechanism by which PLAG1 contributes to reproduction is unknown. To investigate the involvement of PLAG1 in testicular function, we determined (i) the spatial distribution of PLAG1 in the testis using X-gal staining; (ii) transcriptomic consequences of PLAG1 deficiency in knock-out and heterozygous mice compared to wild-type mice using RNA-seq; and (iii) morphological and functional consequences of PLAG1 deficiency by determining testicular histology, daily sperm production and sperm motility in knock-out and wild-type mice. PLAG1 was sparsely expressed in germ cells and in Sertoli cells. Genes known to be involved in spermatogenesis were downregulated in the testes of knock-out mice, as well as Hsd17b3, which encodes a key enzyme in androgen biosynthesis. In the absence of Plag1, a number of genes involved in immune processes and epididymis-specific genes were upregulated in the testes. Finally, loss of PLAG1 resulted in significantly lowered daily sperm production, in reduced sperm motility, and in several animals, in sloughing of the germinal epithelium. Our results demonstrate that the subfertility seen in male PLAG1-deficient mice is, at least in part, the result of significantly reduced sperm output and sperm motility.
format	article
author	Almas R. Juma Sylvia V. H. Grommen Moira K. O’Bryan Anne E. O’Connor D. Jo Merriner Nathan E. Hall Stephen R. Doyle Pauliina E. Damdimopoulou Daniel Barriga Adam H. Hart Wim J. M. Van de Ven Bert De Groef
author_facet	Almas R. Juma Sylvia V. H. Grommen Moira K. O’Bryan Anne E. O’Connor D. Jo Merriner Nathan E. Hall Stephen R. Doyle Pauliina E. Damdimopoulou Daniel Barriga Adam H. Hart Wim J. M. Van de Ven Bert De Groef
author_sort	Almas R. Juma
title	PLAG1 deficiency impairs spermatogenesis and sperm motility in mice
title_short	PLAG1 deficiency impairs spermatogenesis and sperm motility in mice
title_full	PLAG1 deficiency impairs spermatogenesis and sperm motility in mice
title_fullStr	PLAG1 deficiency impairs spermatogenesis and sperm motility in mice
title_full_unstemmed	PLAG1 deficiency impairs spermatogenesis and sperm motility in mice
title_sort	plag1 deficiency impairs spermatogenesis and sperm motility in mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f9a9c5dca672498dbf31f37666823195
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PLAG1 deficiency impairs spermatogenesis and sperm motility in mice

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