Cellular and network contributions to excitability of layer 5 neocortical pyramidal neurons in the rat.

There is a considerable gap between investigating the dynamics of single neurons and the computational aspects of neural networks. A growing number of studies have attempted to overcome this gap using the excitation in brain slices elicited by various chemical manipulations of the bath solution. How...

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Autores principales:	Dan Bar-Yehuda, Alon Korngreen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9bbfe905bdc48c788ac5819ca4723d7
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spelling	oai:doaj.org-article:f9bbfe905bdc48c788ac5819ca4723d72021-11-25T06:13:50ZCellular and network contributions to excitability of layer 5 neocortical pyramidal neurons in the rat.1932-620310.1371/journal.pone.0001209https://doaj.org/article/f9bbfe905bdc48c788ac5819ca4723d72007-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0001209https://doaj.org/toc/1932-6203There is a considerable gap between investigating the dynamics of single neurons and the computational aspects of neural networks. A growing number of studies have attempted to overcome this gap using the excitation in brain slices elicited by various chemical manipulations of the bath solution. However, there has been no quantitative study on the effects of these manipulations on the cellular and network factors controlling excitability. Using the whole-cell configuration of the patch-clamp technique we recorded the membrane potential from the soma of layer 5 pyramidal neurons in acute brain slices from the somatosensory cortex of young rats at 22 degrees C and 35 degrees C. Using blockers of synaptic transmission, we show distinct changes in cellular properties following modification of the ionic composition of the artificial cerebrospinal fluid (ACSF). Thus both cellular and network changes may contribute to the observed effects of slice excitation solutions on the physiology of single neurons. Furthermore, our data suggest that the difference in the ionic composition of current standard ACSF from that of CSF measured in vivo cause ACSF to depress network activity in acute brain slices. This may affect outcomes of experiments investigating biophysical and physiological properties of neurons in such preparations. Our results strongly advocate the necessity of redesigning experiments routinely carried out in the quiescent acute brain slice preparation.Dan Bar-YehudaAlon KorngreenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 2, Iss 11, p e1209 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dan Bar-Yehuda Alon Korngreen Cellular and network contributions to excitability of layer 5 neocortical pyramidal neurons in the rat.
description	There is a considerable gap between investigating the dynamics of single neurons and the computational aspects of neural networks. A growing number of studies have attempted to overcome this gap using the excitation in brain slices elicited by various chemical manipulations of the bath solution. However, there has been no quantitative study on the effects of these manipulations on the cellular and network factors controlling excitability. Using the whole-cell configuration of the patch-clamp technique we recorded the membrane potential from the soma of layer 5 pyramidal neurons in acute brain slices from the somatosensory cortex of young rats at 22 degrees C and 35 degrees C. Using blockers of synaptic transmission, we show distinct changes in cellular properties following modification of the ionic composition of the artificial cerebrospinal fluid (ACSF). Thus both cellular and network changes may contribute to the observed effects of slice excitation solutions on the physiology of single neurons. Furthermore, our data suggest that the difference in the ionic composition of current standard ACSF from that of CSF measured in vivo cause ACSF to depress network activity in acute brain slices. This may affect outcomes of experiments investigating biophysical and physiological properties of neurons in such preparations. Our results strongly advocate the necessity of redesigning experiments routinely carried out in the quiescent acute brain slice preparation.
format	article
author	Dan Bar-Yehuda Alon Korngreen
author_facet	Dan Bar-Yehuda Alon Korngreen
author_sort	Dan Bar-Yehuda
title	Cellular and network contributions to excitability of layer 5 neocortical pyramidal neurons in the rat.
title_short	Cellular and network contributions to excitability of layer 5 neocortical pyramidal neurons in the rat.
title_full	Cellular and network contributions to excitability of layer 5 neocortical pyramidal neurons in the rat.
title_fullStr	Cellular and network contributions to excitability of layer 5 neocortical pyramidal neurons in the rat.
title_full_unstemmed	Cellular and network contributions to excitability of layer 5 neocortical pyramidal neurons in the rat.
title_sort	cellular and network contributions to excitability of layer 5 neocortical pyramidal neurons in the rat.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/f9bbfe905bdc48c788ac5819ca4723d7
work_keys_str_mv	AT danbaryehuda cellularandnetworkcontributionstoexcitabilityoflayer5neocorticalpyramidalneuronsintherat AT alonkorngreen cellularandnetworkcontributionstoexcitabilityoflayer5neocorticalpyramidalneuronsintherat
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Cellular and network contributions to excitability of layer 5 neocortical pyramidal neurons in the rat.

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