Clinical utility of subgingival plaque-specific bacteria in salivary microbiota for detecting periodontitis.

Saliva contains diverse bacteria shed from various oral sites, including subgingival plaque. It is reasonable to focus on the total occupancy of subgingival plaque-specific bacteria (SUBP bacteria), which live in subgingival environments, in the saliva for detecting periodontitis using salivary test...

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Autores principales:	Jiale Ma, Shinya Kageyama, Toru Takeshita, Yukie Shibata, Michiko Furuta, Mikari Asakawa, Yoshihisa Yamashita
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9bcaa50a799497c9eeeac18d03d88f6
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spelling	oai:doaj.org-article:f9bcaa50a799497c9eeeac18d03d88f62021-12-02T20:09:59ZClinical utility of subgingival plaque-specific bacteria in salivary microbiota for detecting periodontitis.1932-620310.1371/journal.pone.0253502https://doaj.org/article/f9bcaa50a799497c9eeeac18d03d88f62021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253502https://doaj.org/toc/1932-6203Saliva contains diverse bacteria shed from various oral sites, including subgingival plaque. It is reasonable to focus on the total occupancy of subgingival plaque-specific bacteria (SUBP bacteria), which live in subgingival environments, in the saliva for detecting periodontitis using salivary testing. This study aimed to validate the clinical utility of SUBP bacteria in the salivary microbiota for the detection of periodontitis. We examined stimulated saliva samples collected from 125 subjects who visited three dental clinics. The relative abundances of previously identified 11 SUBP bacteria were determined using 16S ribosomal RNA gene sequencing and a reference-based approach. The prediction performance was evaluated using a receiver operating characteristic (ROC) curve. The SUBP bacteria accounted for 0-15.4% of the salivary microbiota, and the percentage distinguished periodontitis patients with at least 15 sites with probing depth ≥4 mm with a sensitivity of 0.90 (95% confidence interval [CI], 0.81-0.98) and specificity of 0.70 (95% CI, 0.60-0.80) (area under the ROC curve [AUC], 0.87). Among 2,047 combinations of 11 SUBP bacteria, combinations including Streptococcus constellatus, Porphyromonas gingivalis, and Fusobacterium nucleatum subsp. vincentii demonstrated significantly higher AUC values in their detection. These results suggest that examining SUBP bacteria in saliva may be useful for detecting periodontitis patients in mass screening.Jiale MaShinya KageyamaToru TakeshitaYukie ShibataMichiko FurutaMikari AsakawaYoshihisa YamashitaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253502 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiale Ma Shinya Kageyama Toru Takeshita Yukie Shibata Michiko Furuta Mikari Asakawa Yoshihisa Yamashita Clinical utility of subgingival plaque-specific bacteria in salivary microbiota for detecting periodontitis.
description	Saliva contains diverse bacteria shed from various oral sites, including subgingival plaque. It is reasonable to focus on the total occupancy of subgingival plaque-specific bacteria (SUBP bacteria), which live in subgingival environments, in the saliva for detecting periodontitis using salivary testing. This study aimed to validate the clinical utility of SUBP bacteria in the salivary microbiota for the detection of periodontitis. We examined stimulated saliva samples collected from 125 subjects who visited three dental clinics. The relative abundances of previously identified 11 SUBP bacteria were determined using 16S ribosomal RNA gene sequencing and a reference-based approach. The prediction performance was evaluated using a receiver operating characteristic (ROC) curve. The SUBP bacteria accounted for 0-15.4% of the salivary microbiota, and the percentage distinguished periodontitis patients with at least 15 sites with probing depth ≥4 mm with a sensitivity of 0.90 (95% confidence interval [CI], 0.81-0.98) and specificity of 0.70 (95% CI, 0.60-0.80) (area under the ROC curve [AUC], 0.87). Among 2,047 combinations of 11 SUBP bacteria, combinations including Streptococcus constellatus, Porphyromonas gingivalis, and Fusobacterium nucleatum subsp. vincentii demonstrated significantly higher AUC values in their detection. These results suggest that examining SUBP bacteria in saliva may be useful for detecting periodontitis patients in mass screening.
format	article
author	Jiale Ma Shinya Kageyama Toru Takeshita Yukie Shibata Michiko Furuta Mikari Asakawa Yoshihisa Yamashita
author_facet	Jiale Ma Shinya Kageyama Toru Takeshita Yukie Shibata Michiko Furuta Mikari Asakawa Yoshihisa Yamashita
author_sort	Jiale Ma
title	Clinical utility of subgingival plaque-specific bacteria in salivary microbiota for detecting periodontitis.
title_short	Clinical utility of subgingival plaque-specific bacteria in salivary microbiota for detecting periodontitis.
title_full	Clinical utility of subgingival plaque-specific bacteria in salivary microbiota for detecting periodontitis.
title_fullStr	Clinical utility of subgingival plaque-specific bacteria in salivary microbiota for detecting periodontitis.
title_full_unstemmed	Clinical utility of subgingival plaque-specific bacteria in salivary microbiota for detecting periodontitis.
title_sort	clinical utility of subgingival plaque-specific bacteria in salivary microbiota for detecting periodontitis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f9bcaa50a799497c9eeeac18d03d88f6
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Clinical utility of subgingival plaque-specific bacteria in salivary microbiota for detecting periodontitis.

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