Estimation of bacterial number in the water, sediment and biofilm of Badek and Mewek River (Malang, Indonesia) using Plate Count and eDNA Method

Abstract. Ulinuha D, Andayani S, Hertika AMS, Kilawati Y. 2020. Estimation of bacterial number in the water, sediment, and biofilm of Badek and Mewek River (Malang, Indonesia) using Plate Count and eDNA Method. Biodiversitas 21: 3832-3836. Several methods have been developed to quantify bacterial n...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Devi Ulinuha, Sri Andayani, Asus Maizar Suryanto Hertika, Yuni Kilawati
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	estimation, bacterial number, plate count method, edna method, river Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f9bdcd2d820247df8d9362a804381f12
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f9bdcd2d820247df8d9362a804381f12
record_format	dspace
spelling	oai:doaj.org-article:f9bdcd2d820247df8d9362a804381f122021-11-22T12:17:57ZEstimation of bacterial number in the water, sediment and biofilm of Badek and Mewek River (Malang, Indonesia) using Plate Count and eDNA Method1412-033X2085-472210.13057/biodiv/d210852https://doaj.org/article/f9bdcd2d820247df8d9362a804381f122020-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6334https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Ulinuha D, Andayani S, Hertika AMS, Kilawati Y. 2020. Estimation of bacterial number in the water, sediment, and biofilm of Badek and Mewek River (Malang, Indonesia) using Plate Count and eDNA Method. Biodiversitas 21: 3832-3836. Several methods have been developed to quantify bacterial numbers in the environment, but the studies is still continued to provide the most effective and efficient method. This study was aimed to compare the capacities of two different methods (Plate Count and eDNA Method) to estimate the bacterial number in the water, sediment, and biofilm of Badek and Mewek River (Malang-Indonesia). The Plate Count Method was carried out on a Plate Count Agar (PCA) which was incubated for 24 hours at 37oC. The eDNA method was performed by slow agitation (1,800 rpm) to extract the environmental DNA of bacteria. Quantification of bacteria was obtained from the eDNA band intensity after electrophoresis in 1% gel agarose electrophoresis. Smartladder was used as a marker for the eDNA Method. The result showed that the eDNA Method was efficient for the estimation of bacterial numbers in the sediment and biofilm of Badek and Mewek River. However, this method was not suitable for estimating bacterial numbers in the water.Devi UlinuhaSri AndayaniAsus Maizar Suryanto HertikaYuni KilawatiMBI & UNS Soloarticleestimation, bacterial number, plate count method, edna method, riverBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	estimation, bacterial number, plate count method, edna method, river Biology (General) QH301-705.5
spellingShingle	estimation, bacterial number, plate count method, edna method, river Biology (General) QH301-705.5 Devi Ulinuha Sri Andayani Asus Maizar Suryanto Hertika Yuni Kilawati Estimation of bacterial number in the water, sediment and biofilm of Badek and Mewek River (Malang, Indonesia) using Plate Count and eDNA Method
description	Abstract. Ulinuha D, Andayani S, Hertika AMS, Kilawati Y. 2020. Estimation of bacterial number in the water, sediment, and biofilm of Badek and Mewek River (Malang, Indonesia) using Plate Count and eDNA Method. Biodiversitas 21: 3832-3836. Several methods have been developed to quantify bacterial numbers in the environment, but the studies is still continued to provide the most effective and efficient method. This study was aimed to compare the capacities of two different methods (Plate Count and eDNA Method) to estimate the bacterial number in the water, sediment, and biofilm of Badek and Mewek River (Malang-Indonesia). The Plate Count Method was carried out on a Plate Count Agar (PCA) which was incubated for 24 hours at 37oC. The eDNA method was performed by slow agitation (1,800 rpm) to extract the environmental DNA of bacteria. Quantification of bacteria was obtained from the eDNA band intensity after electrophoresis in 1% gel agarose electrophoresis. Smartladder was used as a marker for the eDNA Method. The result showed that the eDNA Method was efficient for the estimation of bacterial numbers in the sediment and biofilm of Badek and Mewek River. However, this method was not suitable for estimating bacterial numbers in the water.
format	article
author	Devi Ulinuha Sri Andayani Asus Maizar Suryanto Hertika Yuni Kilawati
author_facet	Devi Ulinuha Sri Andayani Asus Maizar Suryanto Hertika Yuni Kilawati
author_sort	Devi Ulinuha
title	Estimation of bacterial number in the water, sediment and biofilm of Badek and Mewek River (Malang, Indonesia) using Plate Count and eDNA Method
title_short	Estimation of bacterial number in the water, sediment and biofilm of Badek and Mewek River (Malang, Indonesia) using Plate Count and eDNA Method
title_full	Estimation of bacterial number in the water, sediment and biofilm of Badek and Mewek River (Malang, Indonesia) using Plate Count and eDNA Method
title_fullStr	Estimation of bacterial number in the water, sediment and biofilm of Badek and Mewek River (Malang, Indonesia) using Plate Count and eDNA Method
title_full_unstemmed	Estimation of bacterial number in the water, sediment and biofilm of Badek and Mewek River (Malang, Indonesia) using Plate Count and eDNA Method
title_sort	estimation of bacterial number in the water, sediment and biofilm of badek and mewek river (malang, indonesia) using plate count and edna method
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/f9bdcd2d820247df8d9362a804381f12
work_keys_str_mv	AT deviulinuha estimationofbacterialnumberinthewatersedimentandbiofilmofbadekandmewekrivermalangindonesiausingplatecountandednamethod AT sriandayani estimationofbacterialnumberinthewatersedimentandbiofilmofbadekandmewekrivermalangindonesiausingplatecountandednamethod AT asusmaizarsuryantohertika estimationofbacterialnumberinthewatersedimentandbiofilmofbadekandmewekrivermalangindonesiausingplatecountandednamethod AT yunikilawati estimationofbacterialnumberinthewatersedimentandbiofilmofbadekandmewekrivermalangindonesiausingplatecountandednamethod
_version_	1718417652031946752

Estimation of bacterial number in the water, sediment and biofilm of Badek and Mewek River (Malang, Indonesia) using Plate Count and eDNA Method

Ejemplares similares