Identification of novel genes and pathways regulating SREBP transcriptional activity.

<h4>Background</h4>Lipid metabolism in mammals is orchestrated by a family of transcription factors called sterol regulatory element-binding proteins (SREBPs) that control the expression of genes required for the uptake and synthesis of cholesterol, fatty acids, and triglycerides. SREBPs...

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Autores principales:	Sandipan Chatterjee, Joseph D Szustakowski, Nirmala R Nanguneri, Craig Mickanin, Mark A Labow, Axel Nohturfft, Kumlesh K Dev, Rajeev Sivasankaran
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9c070563cbb4b3cb0db1a17be7bb0ba
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spelling	oai:doaj.org-article:f9c070563cbb4b3cb0db1a17be7bb0ba2021-11-25T06:16:03ZIdentification of novel genes and pathways regulating SREBP transcriptional activity.1932-620310.1371/journal.pone.0005197https://doaj.org/article/f9c070563cbb4b3cb0db1a17be7bb0ba2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19381295/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Lipid metabolism in mammals is orchestrated by a family of transcription factors called sterol regulatory element-binding proteins (SREBPs) that control the expression of genes required for the uptake and synthesis of cholesterol, fatty acids, and triglycerides. SREBPs are thus essential for insulin-induced lipogenesis and for cellular membrane homeostasis and biogenesis. Although multiple players have been identified that control the expression and activation of SREBPs, gaps remain in our understanding of how SREBPs are coordinated with other physiological pathways.<h4>Methodology</h4>To identify novel regulators of SREBPs, we performed a genome-wide cDNA over-expression screen to identify proteins that might modulate the transcription of a luciferase gene driven from an SREBP-specific promoter. The results were verified through secondary biological assays and expression data were analyzed by a novel application of the Gene Set Enrichment Analysis (GSEA) method.<h4>Conclusions/significance</h4>We screened 10,000 different cDNAs and identified a number of genes and pathways that have previously not been implicated in SREBP control and cellular cholesterol homeostasis. These findings further our understanding of lipid biology and should lead to new insights into lipid associated disorders.Sandipan ChatterjeeJoseph D SzustakowskiNirmala R NanguneriCraig MickaninMark A LabowAxel NohturfftKumlesh K DevRajeev SivasankaranPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 4, p e5197 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sandipan Chatterjee Joseph D Szustakowski Nirmala R Nanguneri Craig Mickanin Mark A Labow Axel Nohturfft Kumlesh K Dev Rajeev Sivasankaran Identification of novel genes and pathways regulating SREBP transcriptional activity.
description	<h4>Background</h4>Lipid metabolism in mammals is orchestrated by a family of transcription factors called sterol regulatory element-binding proteins (SREBPs) that control the expression of genes required for the uptake and synthesis of cholesterol, fatty acids, and triglycerides. SREBPs are thus essential for insulin-induced lipogenesis and for cellular membrane homeostasis and biogenesis. Although multiple players have been identified that control the expression and activation of SREBPs, gaps remain in our understanding of how SREBPs are coordinated with other physiological pathways.<h4>Methodology</h4>To identify novel regulators of SREBPs, we performed a genome-wide cDNA over-expression screen to identify proteins that might modulate the transcription of a luciferase gene driven from an SREBP-specific promoter. The results were verified through secondary biological assays and expression data were analyzed by a novel application of the Gene Set Enrichment Analysis (GSEA) method.<h4>Conclusions/significance</h4>We screened 10,000 different cDNAs and identified a number of genes and pathways that have previously not been implicated in SREBP control and cellular cholesterol homeostasis. These findings further our understanding of lipid biology and should lead to new insights into lipid associated disorders.
format	article
author	Sandipan Chatterjee Joseph D Szustakowski Nirmala R Nanguneri Craig Mickanin Mark A Labow Axel Nohturfft Kumlesh K Dev Rajeev Sivasankaran
author_facet	Sandipan Chatterjee Joseph D Szustakowski Nirmala R Nanguneri Craig Mickanin Mark A Labow Axel Nohturfft Kumlesh K Dev Rajeev Sivasankaran
author_sort	Sandipan Chatterjee
title	Identification of novel genes and pathways regulating SREBP transcriptional activity.
title_short	Identification of novel genes and pathways regulating SREBP transcriptional activity.
title_full	Identification of novel genes and pathways regulating SREBP transcriptional activity.
title_fullStr	Identification of novel genes and pathways regulating SREBP transcriptional activity.
title_full_unstemmed	Identification of novel genes and pathways regulating SREBP transcriptional activity.
title_sort	identification of novel genes and pathways regulating srebp transcriptional activity.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/f9c070563cbb4b3cb0db1a17be7bb0ba
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Identification of novel genes and pathways regulating SREBP transcriptional activity.

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