Biostimulant Capacity of an Enzymatic Extract From Rice Bran Against Ozone-Induced Damage in Capsicum annum

Ozone is a destructive pollutant, damaging crops, and decreasing crop yield. Therefore, there is great interest in finding strategies to alleviate ozone-induced crop losses. In plants, ozone enters leaves through the stomata and is immediately degraded into reactive oxygen species (ROS), producing R...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sandra Macias-Benitez, Salvadora Navarro-Torre, Pablo Caballero, Luis Martín, Elisa Revilla, Angélica Castaño, Juan Parrado
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	ozone ROS rice bran enzymatic extract MAPK Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/f9c35086efd948718b426d1fdafbeaef
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f9c35086efd948718b426d1fdafbeaef
record_format	dspace
spelling	oai:doaj.org-article:f9c35086efd948718b426d1fdafbeaef2021-11-19T15:21:40ZBiostimulant Capacity of an Enzymatic Extract From Rice Bran Against Ozone-Induced Damage in Capsicum annum1664-462X10.3389/fpls.2021.749422https://doaj.org/article/f9c35086efd948718b426d1fdafbeaef2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.749422/fullhttps://doaj.org/toc/1664-462XOzone is a destructive pollutant, damaging crops, and decreasing crop yield. Therefore, there is great interest in finding strategies to alleviate ozone-induced crop losses. In plants, ozone enters leaves through the stomata and is immediately degraded into reactive oxygen species (ROS), producing ROS stress in plants. ROS stress can be controlled by ROS-scavenging systems that include enzymatic or non-enzymatic mechanisms. Our research group has developed a product from rice bran, a by-product of rice milling which has bioactive molecules that act as an antioxidant compound. This product is a water-soluble rice bran enzymatic extract (RBEE) which preserves all the properties and improves the solubility of proteins and the antioxidant components of rice bran. In previous works, the beneficial properties of RBEE have been demonstrated in animals. However, to date, RBEE has not been used as a protective agent against oxidative damage in agricultural fields. The main goal of this study was to investigate the ability of RBEE to be used as a biostimulant by preventing oxidative damage in plants, after ozone exposure. To perform this investigation, pepper plants (Capsicum annuum) exposed to ozone were treated with RBEE. RBEE protected the ozone-induced damage, as revealed by net photosynthetic rate and the content of photosynthetic pigments. RBEE also decreased the induction of antioxidant enzyme activities in leaves (catalase, superoxide dismutase, and ascorbate peroxidase) due to ozone exposure. ROS generation is a common consequence of diverse cellular traumas that also activate the mitogen-activated protein kinase (MAPK) cascade. Thus, it is known that the ozone damages are triggered by the MAPK cascade. To examine the involvement of the MAPK cascade in the ozone damage CaMPK6-1, CaMPK6-2, and CaMKK5 genes were analyzed by qRT-PCR. The results showed the involvement of the MAPK pathway in both, not only in ozone damage but especially in its protection by RBEE. Taken together, these results support that RBEE protects plants against ozone exposure and its use as a new biostimulant could be proposed.Sandra Macias-BenitezSalvadora Navarro-TorrePablo CaballeroLuis MartínElisa RevillaAngélica CastañoJuan ParradoFrontiers Media S.A.articleozoneROSrice branenzymatic extractMAPKPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ozone ROS rice bran enzymatic extract MAPK Plant culture SB1-1110
spellingShingle	ozone ROS rice bran enzymatic extract MAPK Plant culture SB1-1110 Sandra Macias-Benitez Salvadora Navarro-Torre Pablo Caballero Luis Martín Elisa Revilla Angélica Castaño Juan Parrado Biostimulant Capacity of an Enzymatic Extract From Rice Bran Against Ozone-Induced Damage in Capsicum annum
description	Ozone is a destructive pollutant, damaging crops, and decreasing crop yield. Therefore, there is great interest in finding strategies to alleviate ozone-induced crop losses. In plants, ozone enters leaves through the stomata and is immediately degraded into reactive oxygen species (ROS), producing ROS stress in plants. ROS stress can be controlled by ROS-scavenging systems that include enzymatic or non-enzymatic mechanisms. Our research group has developed a product from rice bran, a by-product of rice milling which has bioactive molecules that act as an antioxidant compound. This product is a water-soluble rice bran enzymatic extract (RBEE) which preserves all the properties and improves the solubility of proteins and the antioxidant components of rice bran. In previous works, the beneficial properties of RBEE have been demonstrated in animals. However, to date, RBEE has not been used as a protective agent against oxidative damage in agricultural fields. The main goal of this study was to investigate the ability of RBEE to be used as a biostimulant by preventing oxidative damage in plants, after ozone exposure. To perform this investigation, pepper plants (Capsicum annuum) exposed to ozone were treated with RBEE. RBEE protected the ozone-induced damage, as revealed by net photosynthetic rate and the content of photosynthetic pigments. RBEE also decreased the induction of antioxidant enzyme activities in leaves (catalase, superoxide dismutase, and ascorbate peroxidase) due to ozone exposure. ROS generation is a common consequence of diverse cellular traumas that also activate the mitogen-activated protein kinase (MAPK) cascade. Thus, it is known that the ozone damages are triggered by the MAPK cascade. To examine the involvement of the MAPK cascade in the ozone damage CaMPK6-1, CaMPK6-2, and CaMKK5 genes were analyzed by qRT-PCR. The results showed the involvement of the MAPK pathway in both, not only in ozone damage but especially in its protection by RBEE. Taken together, these results support that RBEE protects plants against ozone exposure and its use as a new biostimulant could be proposed.
format	article
author	Sandra Macias-Benitez Salvadora Navarro-Torre Pablo Caballero Luis Martín Elisa Revilla Angélica Castaño Juan Parrado
author_facet	Sandra Macias-Benitez Salvadora Navarro-Torre Pablo Caballero Luis Martín Elisa Revilla Angélica Castaño Juan Parrado
author_sort	Sandra Macias-Benitez
title	Biostimulant Capacity of an Enzymatic Extract From Rice Bran Against Ozone-Induced Damage in Capsicum annum
title_short	Biostimulant Capacity of an Enzymatic Extract From Rice Bran Against Ozone-Induced Damage in Capsicum annum
title_full	Biostimulant Capacity of an Enzymatic Extract From Rice Bran Against Ozone-Induced Damage in Capsicum annum
title_fullStr	Biostimulant Capacity of an Enzymatic Extract From Rice Bran Against Ozone-Induced Damage in Capsicum annum
title_full_unstemmed	Biostimulant Capacity of an Enzymatic Extract From Rice Bran Against Ozone-Induced Damage in Capsicum annum
title_sort	biostimulant capacity of an enzymatic extract from rice bran against ozone-induced damage in capsicum annum
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f9c35086efd948718b426d1fdafbeaef
work_keys_str_mv	AT sandramaciasbenitez biostimulantcapacityofanenzymaticextractfromricebranagainstozoneinduceddamageincapsicumannum AT salvadoranavarrotorre biostimulantcapacityofanenzymaticextractfromricebranagainstozoneinduceddamageincapsicumannum AT pablocaballero biostimulantcapacityofanenzymaticextractfromricebranagainstozoneinduceddamageincapsicumannum AT luismartin biostimulantcapacityofanenzymaticextractfromricebranagainstozoneinduceddamageincapsicumannum AT elisarevilla biostimulantcapacityofanenzymaticextractfromricebranagainstozoneinduceddamageincapsicumannum AT angelicacastano biostimulantcapacityofanenzymaticextractfromricebranagainstozoneinduceddamageincapsicumannum AT juanparrado biostimulantcapacityofanenzymaticextractfromricebranagainstozoneinduceddamageincapsicumannum
_version_	1718420047923249152

Biostimulant Capacity of an Enzymatic Extract From Rice Bran Against Ozone-Induced Damage in Capsicum annum

Ejemplares similares