Identification of an operon involved in fluoride resistance in Enterobacter cloacae FRM

Abstract Fluorine is ubiquitous and the most active non-metal element in nature. While many microorganisms have developed fluoride resistance as a result of the widespread and prolonged application of oral hygiene products, the mechanisms used by these organisms to overcome fluoride toxicity are inc...

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Autores principales:	Xiaoqing Liu, Jian Tian, Lihui Liu, Tao Zhu, Xiaoxia Yu, Xiaoyu Chu, Bin Yao, Ningfeng Wu, Yunliu Fan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9d87b22469d4124b11c9b103951dc36
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spelling	oai:doaj.org-article:f9d87b22469d4124b11c9b103951dc362021-12-02T15:05:41ZIdentification of an operon involved in fluoride resistance in Enterobacter cloacae FRM10.1038/s41598-017-06988-12045-2322https://doaj.org/article/f9d87b22469d4124b11c9b103951dc362017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06988-1https://doaj.org/toc/2045-2322Abstract Fluorine is ubiquitous and the most active non-metal element in nature. While many microorganisms have developed fluoride resistance as a result of the widespread and prolonged application of oral hygiene products, the mechanisms used by these organisms to overcome fluoride toxicity are incompletely understood. In this study, a fluoride-resistant strain, Enterobacter cloacae FRM, was identified which could grow well at a fluoride concentration of 4,000 mg/L. According to comparative genomics, transcriptome under fluoride stress, and sequence analyses of two fluoride-resistant fosmid clones, the genomic island GI3 was found to be important for fluoride resistance. The result of quantitative RT-PCR indicated that six genes on GI3, ppaC, uspA, eno, gpmA, crcB, and orf5249, which encode a fluoride transporter, fluoride-inhibited enzymes, and a universal stress protein, reside in an operon and are transcribed into two mRNAs activated by fluoride with a fluoride riboswitch. The results of knockout and complementation experiments indicated that these genes work together to provide high fluoride resistance to E. cloacae FRM. This study clarified the resistance mechanism of this high fluoride-resistant organism and has expanded our understanding of the biological effects of fluoride.Xiaoqing LiuJian TianLihui LiuTao ZhuXiaoxia YuXiaoyu ChuBin YaoNingfeng WuYunliu FanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoqing Liu Jian Tian Lihui Liu Tao Zhu Xiaoxia Yu Xiaoyu Chu Bin Yao Ningfeng Wu Yunliu Fan Identification of an operon involved in fluoride resistance in Enterobacter cloacae FRM
description	Abstract Fluorine is ubiquitous and the most active non-metal element in nature. While many microorganisms have developed fluoride resistance as a result of the widespread and prolonged application of oral hygiene products, the mechanisms used by these organisms to overcome fluoride toxicity are incompletely understood. In this study, a fluoride-resistant strain, Enterobacter cloacae FRM, was identified which could grow well at a fluoride concentration of 4,000 mg/L. According to comparative genomics, transcriptome under fluoride stress, and sequence analyses of two fluoride-resistant fosmid clones, the genomic island GI3 was found to be important for fluoride resistance. The result of quantitative RT-PCR indicated that six genes on GI3, ppaC, uspA, eno, gpmA, crcB, and orf5249, which encode a fluoride transporter, fluoride-inhibited enzymes, and a universal stress protein, reside in an operon and are transcribed into two mRNAs activated by fluoride with a fluoride riboswitch. The results of knockout and complementation experiments indicated that these genes work together to provide high fluoride resistance to E. cloacae FRM. This study clarified the resistance mechanism of this high fluoride-resistant organism and has expanded our understanding of the biological effects of fluoride.
format	article
author	Xiaoqing Liu Jian Tian Lihui Liu Tao Zhu Xiaoxia Yu Xiaoyu Chu Bin Yao Ningfeng Wu Yunliu Fan
author_facet	Xiaoqing Liu Jian Tian Lihui Liu Tao Zhu Xiaoxia Yu Xiaoyu Chu Bin Yao Ningfeng Wu Yunliu Fan
author_sort	Xiaoqing Liu
title	Identification of an operon involved in fluoride resistance in Enterobacter cloacae FRM
title_short	Identification of an operon involved in fluoride resistance in Enterobacter cloacae FRM
title_full	Identification of an operon involved in fluoride resistance in Enterobacter cloacae FRM
title_fullStr	Identification of an operon involved in fluoride resistance in Enterobacter cloacae FRM
title_full_unstemmed	Identification of an operon involved in fluoride resistance in Enterobacter cloacae FRM
title_sort	identification of an operon involved in fluoride resistance in enterobacter cloacae frm
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f9d87b22469d4124b11c9b103951dc36
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Identification of an operon involved in fluoride resistance in Enterobacter cloacae FRM

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