Leading individual features of antioxidant systematically classified by the ORAC assay and its single electron transfer and hydrogen atom transfer reactivities; analyzing ALS therapeutic drug Edaravone

Many natural compounds mop up radicals and limit radical reactions and may prove useful in reducing or preventing oxidative stress-related diseases in vivo. Several assays have been developed to measure antioxidant or anti-radical activity. Here, we measured the anti-radical activities of representa...

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Autores principales:	Miwa Takatsuka, Satoru Goto, Kenshiro Kobayashi, Yuta Otsuka, Yohsuke Shimada
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Anti-radical activity Antioxidant Voronoi polyhedral division ORAC analysis Edaravone Biochemistry QD415-436 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/f9da5f0f82004817b1b81e831f02a54e
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spelling	oai:doaj.org-article:f9da5f0f82004817b1b81e831f02a54e2021-11-18T04:53:49ZLeading individual features of antioxidant systematically classified by the ORAC assay and its single electron transfer and hydrogen atom transfer reactivities; analyzing ALS therapeutic drug Edaravone2667-160310.1016/j.bbadva.2021.100030https://doaj.org/article/f9da5f0f82004817b1b81e831f02a54e2021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667160321000296https://doaj.org/toc/2667-1603Many natural compounds mop up radicals and limit radical reactions and may prove useful in reducing or preventing oxidative stress-related diseases in vivo. Several assays have been developed to measure antioxidant or anti-radical activity. Here, we measured the anti-radical activities of representative antioxidants using different assays. The oxygen radical absorption capacity (ORAC) assay has two mechanistic stages. We classified antioxidant behavior using two characteristic values thought to be related to the two stages — peroxyl radical formation time (lag time) and fluorescein annihilation rate (kobs) — by applying Voronoi polyhedral division. We focused on four class-representative antioxidants, Trolox ®, vitamin C, l-cysteine, and 2,6-di‑tert‑butyl‑p-cresol, and compared their characteristic activities with those of edaravone. Our analysis indicates that edaravone is in the same group as cysteine and may function via a similar mechanism. Our results suggest that analyzing lag time and kobs is a useful method to characterize antioxidants.Miwa TakatsukaSatoru GotoKenshiro KobayashiYuta OtsukaYohsuke ShimadaElsevierarticleAnti-radical activityAntioxidantVoronoi polyhedral divisionORAC analysisEdaravoneBiochemistryQD415-436GeneticsQH426-470ENBBA Advances, Vol 1, Iss , Pp 100030- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Anti-radical activity Antioxidant Voronoi polyhedral division ORAC analysis Edaravone Biochemistry QD415-436 Genetics QH426-470
spellingShingle	Anti-radical activity Antioxidant Voronoi polyhedral division ORAC analysis Edaravone Biochemistry QD415-436 Genetics QH426-470 Miwa Takatsuka Satoru Goto Kenshiro Kobayashi Yuta Otsuka Yohsuke Shimada Leading individual features of antioxidant systematically classified by the ORAC assay and its single electron transfer and hydrogen atom transfer reactivities; analyzing ALS therapeutic drug Edaravone
description	Many natural compounds mop up radicals and limit radical reactions and may prove useful in reducing or preventing oxidative stress-related diseases in vivo. Several assays have been developed to measure antioxidant or anti-radical activity. Here, we measured the anti-radical activities of representative antioxidants using different assays. The oxygen radical absorption capacity (ORAC) assay has two mechanistic stages. We classified antioxidant behavior using two characteristic values thought to be related to the two stages — peroxyl radical formation time (lag time) and fluorescein annihilation rate (kobs) — by applying Voronoi polyhedral division. We focused on four class-representative antioxidants, Trolox ®, vitamin C, l-cysteine, and 2,6-di‑tert‑butyl‑p-cresol, and compared their characteristic activities with those of edaravone. Our analysis indicates that edaravone is in the same group as cysteine and may function via a similar mechanism. Our results suggest that analyzing lag time and kobs is a useful method to characterize antioxidants.
format	article
author	Miwa Takatsuka Satoru Goto Kenshiro Kobayashi Yuta Otsuka Yohsuke Shimada
author_facet	Miwa Takatsuka Satoru Goto Kenshiro Kobayashi Yuta Otsuka Yohsuke Shimada
author_sort	Miwa Takatsuka
title	Leading individual features of antioxidant systematically classified by the ORAC assay and its single electron transfer and hydrogen atom transfer reactivities; analyzing ALS therapeutic drug Edaravone
title_short	Leading individual features of antioxidant systematically classified by the ORAC assay and its single electron transfer and hydrogen atom transfer reactivities; analyzing ALS therapeutic drug Edaravone
title_full	Leading individual features of antioxidant systematically classified by the ORAC assay and its single electron transfer and hydrogen atom transfer reactivities; analyzing ALS therapeutic drug Edaravone
title_fullStr	Leading individual features of antioxidant systematically classified by the ORAC assay and its single electron transfer and hydrogen atom transfer reactivities; analyzing ALS therapeutic drug Edaravone
title_full_unstemmed	Leading individual features of antioxidant systematically classified by the ORAC assay and its single electron transfer and hydrogen atom transfer reactivities; analyzing ALS therapeutic drug Edaravone
title_sort	leading individual features of antioxidant systematically classified by the orac assay and its single electron transfer and hydrogen atom transfer reactivities; analyzing als therapeutic drug edaravone
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f9da5f0f82004817b1b81e831f02a54e
work_keys_str_mv	AT miwatakatsuka leadingindividualfeaturesofantioxidantsystematicallyclassifiedbytheoracassayanditssingleelectrontransferandhydrogenatomtransferreactivitiesanalyzingalstherapeuticdrugedaravone AT satorugoto leadingindividualfeaturesofantioxidantsystematicallyclassifiedbytheoracassayanditssingleelectrontransferandhydrogenatomtransferreactivitiesanalyzingalstherapeuticdrugedaravone AT kenshirokobayashi leadingindividualfeaturesofantioxidantsystematicallyclassifiedbytheoracassayanditssingleelectrontransferandhydrogenatomtransferreactivitiesanalyzingalstherapeuticdrugedaravone AT yutaotsuka leadingindividualfeaturesofantioxidantsystematicallyclassifiedbytheoracassayanditssingleelectrontransferandhydrogenatomtransferreactivitiesanalyzingalstherapeuticdrugedaravone AT yohsukeshimada leadingindividualfeaturesofantioxidantsystematicallyclassifiedbytheoracassayanditssingleelectrontransferandhydrogenatomtransferreactivitiesanalyzingalstherapeuticdrugedaravone
_version_	1718424952311382016

Leading individual features of antioxidant systematically classified by the ORAC assay and its single electron transfer and hydrogen atom transfer reactivities; analyzing ALS therapeutic drug Edaravone

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