Molecular characterization of Fe-acquisition genes causing decreased Fe uptake and photosynthetic inefficiency in Fe-deficient sunflower

Abstract Iron (Fe) deficiency in plants hinders growth and yield. Thus, this study aims to elucidate the responses and molecular characterization of genes in Fe-deficient sunflower. The study was conducted on 14 days-old sunflower plants cultivated in hydroponic culture under Fe-sufficient and Fe-de...

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Autores principales:	Ahmad Humayan Kabir, Sharaban Tahura, Mona M. Elseehy, Ahmed M. El-Shehawi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9dfd2cea2b544d4bdcc08d11350f589
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spelling	oai:doaj.org-article:f9dfd2cea2b544d4bdcc08d11350f5892021-12-02T15:54:02ZMolecular characterization of Fe-acquisition genes causing decreased Fe uptake and photosynthetic inefficiency in Fe-deficient sunflower10.1038/s41598-021-85147-z2045-2322https://doaj.org/article/f9dfd2cea2b544d4bdcc08d11350f5892021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85147-zhttps://doaj.org/toc/2045-2322Abstract Iron (Fe) deficiency in plants hinders growth and yield. Thus, this study aims to elucidate the responses and molecular characterization of genes in Fe-deficient sunflower. The study was conducted on 14 days-old sunflower plants cultivated in hydroponic culture under Fe-sufficient and Fe-deficient conditions. The Fe-starved sunflower showed substantial decrease in plant biomass, SPAD score, quantum yield efficiency of PSII (Fv/Fm), photosynthetic performance index (Pi_ABS). Further, Fe shortage reduced Fe and Zn concentrations in roots and shoots, accompanied by a marked decrease of HaNramp1 and HaZIP1 expression in roots, suggesting the association of Zn status contributing to photosynthetic inefficiency in sunflower. The ferric chelate reductase (FCR) activity, along with HaFRO2 and HaIRT1 transcripts, were constitutively expressed, suggesting that sunflower plants can regulate FCR activity, although the lack of bioavailable Fe in the rhizosphere strongly corresponds to the limited Fe uptake in sunflower. The substantial increase of proton extrusion in roots and the localization of Fe-related genes in the plasma membrane are also evident in sunflower as common responses to Fe-deficiency by this Strategy I plant species. Analysis showed that three motifs of Fe-related proteins were linked to the ZIP zinc transporter. The interactome map revealed the close partnership of these Fe-related genes in addition to FRU gene encoding putative transcription factor linked to Fe uptake response. The cis-regulatory analysis of promoter suggested the involvement of auxin, salicylic acid, and methyl jasmonate-responsive elements in the regulatory process in response to Fe deficiency. These findings may be beneficial to develop Fe-efficient sunflower plants through breeding or genome editing approaches.Ahmad Humayan KabirSharaban TahuraMona M. ElseehyAhmed M. El-ShehawiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ahmad Humayan Kabir Sharaban Tahura Mona M. Elseehy Ahmed M. El-Shehawi Molecular characterization of Fe-acquisition genes causing decreased Fe uptake and photosynthetic inefficiency in Fe-deficient sunflower
description	Abstract Iron (Fe) deficiency in plants hinders growth and yield. Thus, this study aims to elucidate the responses and molecular characterization of genes in Fe-deficient sunflower. The study was conducted on 14 days-old sunflower plants cultivated in hydroponic culture under Fe-sufficient and Fe-deficient conditions. The Fe-starved sunflower showed substantial decrease in plant biomass, SPAD score, quantum yield efficiency of PSII (Fv/Fm), photosynthetic performance index (Pi_ABS). Further, Fe shortage reduced Fe and Zn concentrations in roots and shoots, accompanied by a marked decrease of HaNramp1 and HaZIP1 expression in roots, suggesting the association of Zn status contributing to photosynthetic inefficiency in sunflower. The ferric chelate reductase (FCR) activity, along with HaFRO2 and HaIRT1 transcripts, were constitutively expressed, suggesting that sunflower plants can regulate FCR activity, although the lack of bioavailable Fe in the rhizosphere strongly corresponds to the limited Fe uptake in sunflower. The substantial increase of proton extrusion in roots and the localization of Fe-related genes in the plasma membrane are also evident in sunflower as common responses to Fe-deficiency by this Strategy I plant species. Analysis showed that three motifs of Fe-related proteins were linked to the ZIP zinc transporter. The interactome map revealed the close partnership of these Fe-related genes in addition to FRU gene encoding putative transcription factor linked to Fe uptake response. The cis-regulatory analysis of promoter suggested the involvement of auxin, salicylic acid, and methyl jasmonate-responsive elements in the regulatory process in response to Fe deficiency. These findings may be beneficial to develop Fe-efficient sunflower plants through breeding or genome editing approaches.
format	article
author	Ahmad Humayan Kabir Sharaban Tahura Mona M. Elseehy Ahmed M. El-Shehawi
author_facet	Ahmad Humayan Kabir Sharaban Tahura Mona M. Elseehy Ahmed M. El-Shehawi
author_sort	Ahmad Humayan Kabir
title	Molecular characterization of Fe-acquisition genes causing decreased Fe uptake and photosynthetic inefficiency in Fe-deficient sunflower
title_short	Molecular characterization of Fe-acquisition genes causing decreased Fe uptake and photosynthetic inefficiency in Fe-deficient sunflower
title_full	Molecular characterization of Fe-acquisition genes causing decreased Fe uptake and photosynthetic inefficiency in Fe-deficient sunflower
title_fullStr	Molecular characterization of Fe-acquisition genes causing decreased Fe uptake and photosynthetic inefficiency in Fe-deficient sunflower
title_full_unstemmed	Molecular characterization of Fe-acquisition genes causing decreased Fe uptake and photosynthetic inefficiency in Fe-deficient sunflower
title_sort	molecular characterization of fe-acquisition genes causing decreased fe uptake and photosynthetic inefficiency in fe-deficient sunflower
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f9dfd2cea2b544d4bdcc08d11350f589
work_keys_str_mv	AT ahmadhumayankabir molecularcharacterizationoffeacquisitiongenescausingdecreasedfeuptakeandphotosyntheticinefficiencyinfedeficientsunflower AT sharabantahura molecularcharacterizationoffeacquisitiongenescausingdecreasedfeuptakeandphotosyntheticinefficiencyinfedeficientsunflower AT monamelseehy molecularcharacterizationoffeacquisitiongenescausingdecreasedfeuptakeandphotosyntheticinefficiencyinfedeficientsunflower AT ahmedmelshehawi molecularcharacterizationoffeacquisitiongenescausingdecreasedfeuptakeandphotosyntheticinefficiencyinfedeficientsunflower
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Molecular characterization of Fe-acquisition genes causing decreased Fe uptake and photosynthetic inefficiency in Fe-deficient sunflower

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