The Role of 20-HETE, COX, Thromboxane Receptors, and Blood Plasma Antioxidant Status in Vascular Relaxation of Copper-Nanoparticle-Fed WKY Rats
Recently, the addition of copper nanoparticles (NPs) in a daily diet (6.5 mg/kg) was studied in different animal models as a possible alternative to ionic forms. Male Wistar–Kyoto rats (24-week-old, <i>n</i> = 11) were fed with copper, either in the form of carbonate salt (Cu<sub>6...
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| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/f9e467905e73469ca6a67df89ac9d40c |
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| Sumario: | Recently, the addition of copper nanoparticles (NPs) in a daily diet (6.5 mg/kg) was studied in different animal models as a possible alternative to ionic forms. Male Wistar–Kyoto rats (24-week-old, <i>n</i> = 11) were fed with copper, either in the form of carbonate salt (Cu<sub>6.5</sub>) or metal-based copper NPs (NP<sub>6.5</sub>), for 8 weeks. The third group was fed with a half dose of each (NP<sub>3.25</sub> + Cu<sub>3.25</sub>). The thoracic aorta and blood plasma was studied. Supplementation with NP<sub>6.5</sub> decreased the Cu (×0.7), Cu/Zn-ratio (×0.6) and catalase (CAT, ×0.7), and increased Zn (×1.2) and superoxide dismutase (SOD, ×1.4). Meanwhile, NP<sub>3.25</sub> + Cu<sub>3.25</sub> decreased the Cu/Zn-ratio (×0.7), and CAT (×0.7), and increased the daily feed intake (×1.06). Preincubation with either the selective cyclooxygenase (COX)-2 inhibitor, or the non-selective COX-1/2 inhibitor attenuated vasodilation of rat thoracic aorta in the NP<sub>6.5</sub> group exclusively. However, an increased vasodilator response was observed in the NP<sub>6.5</sub> and NP<sub>3.25</sub> + Cu<sub>3.25</sub> group of rats after preincubation with an inhibitor of 20-hydroxyeicosatetraenoic acid (20-HETE) formation, and the thromboxane receptor (TP) antagonist. Significant differences were observed between the NP<sub>6.5</sub> and NP<sub>3.25</sub> + Cu<sub>3.25</sub> groups of rats in: dietary intake, acetylcholine-induced vasodilation, and response to COX-inhibitors. Copper NPs in a standard daily dose had more significant effects on the mechanism(s) responsible for the utilization of reactive oxygen species in the blood plasma with the participation of prostanoids derived from COX-2 in the vascular relaxation. Dietary copper NPs in both doses modified vasodilation through the vasoconstrictor 20-HETE and the TP receptors. |
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