Mitochondrial phosphate transporter and methyltransferase genes contribute to Fusarium head blight Type II disease resistance and grain development in wheat.

Fusarium head blight (FHB) is an economically important disease of wheat that results in yield loss and grain contaminated with fungal mycotoxins that are harmful to human and animal health. Herein we characterised two wheat genes involved in the FHB response in wheat: a wheat mitochondrial phosphat...

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Autores principales:	Keshav B Malla, Ganesh Thapa, Fiona M Doohan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9e7e7485b004b68bc09cabab4febf18
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spelling	oai:doaj.org-article:f9e7e7485b004b68bc09cabab4febf182021-12-02T20:16:53ZMitochondrial phosphate transporter and methyltransferase genes contribute to Fusarium head blight Type II disease resistance and grain development in wheat.1932-620310.1371/journal.pone.0258726https://doaj.org/article/f9e7e7485b004b68bc09cabab4febf182021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258726https://doaj.org/toc/1932-6203Fusarium head blight (FHB) is an economically important disease of wheat that results in yield loss and grain contaminated with fungal mycotoxins that are harmful to human and animal health. Herein we characterised two wheat genes involved in the FHB response in wheat: a wheat mitochondrial phosphate transporter (TaMPT) and a methyltransferase (TaSAM). Wheat has three sub-genomes (A, B, and D) and gene expression studies demonstrated that TaMPT and TaSAM homoeologs were differentially expressed in response to FHB infection and the mycotoxigenic Fusarium virulence factor deoxynivalenol (DON) in FHB resistant wheat cv. CM82036 and susceptible cv. Remus. Virus-induced gene silencing (VIGS) of either TaMPT or TaSAM enhanced the susceptibility of cv. CM82036 to FHB disease, reducing disease spread (Type II disease resistance). VIGS of TaMPT and TaSAM significantly reduced grain number and grain weight. This indicates TaSAM and TaMPT genes also contribute to grain development in wheat and adds to the increasing body of evidence linking FHB resistance genes to grain development. Hence, Fusarium responsive genes TaSAM and TaMPT warrant further study to determine their potential to enhance both disease resistance and grain development in wheat.Keshav B MallaGanesh ThapaFiona M DoohanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258726 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keshav B Malla Ganesh Thapa Fiona M Doohan Mitochondrial phosphate transporter and methyltransferase genes contribute to Fusarium head blight Type II disease resistance and grain development in wheat.
description	Fusarium head blight (FHB) is an economically important disease of wheat that results in yield loss and grain contaminated with fungal mycotoxins that are harmful to human and animal health. Herein we characterised two wheat genes involved in the FHB response in wheat: a wheat mitochondrial phosphate transporter (TaMPT) and a methyltransferase (TaSAM). Wheat has three sub-genomes (A, B, and D) and gene expression studies demonstrated that TaMPT and TaSAM homoeologs were differentially expressed in response to FHB infection and the mycotoxigenic Fusarium virulence factor deoxynivalenol (DON) in FHB resistant wheat cv. CM82036 and susceptible cv. Remus. Virus-induced gene silencing (VIGS) of either TaMPT or TaSAM enhanced the susceptibility of cv. CM82036 to FHB disease, reducing disease spread (Type II disease resistance). VIGS of TaMPT and TaSAM significantly reduced grain number and grain weight. This indicates TaSAM and TaMPT genes also contribute to grain development in wheat and adds to the increasing body of evidence linking FHB resistance genes to grain development. Hence, Fusarium responsive genes TaSAM and TaMPT warrant further study to determine their potential to enhance both disease resistance and grain development in wheat.
format	article
author	Keshav B Malla Ganesh Thapa Fiona M Doohan
author_facet	Keshav B Malla Ganesh Thapa Fiona M Doohan
author_sort	Keshav B Malla
title	Mitochondrial phosphate transporter and methyltransferase genes contribute to Fusarium head blight Type II disease resistance and grain development in wheat.
title_short	Mitochondrial phosphate transporter and methyltransferase genes contribute to Fusarium head blight Type II disease resistance and grain development in wheat.
title_full	Mitochondrial phosphate transporter and methyltransferase genes contribute to Fusarium head blight Type II disease resistance and grain development in wheat.
title_fullStr	Mitochondrial phosphate transporter and methyltransferase genes contribute to Fusarium head blight Type II disease resistance and grain development in wheat.
title_full_unstemmed	Mitochondrial phosphate transporter and methyltransferase genes contribute to Fusarium head blight Type II disease resistance and grain development in wheat.
title_sort	mitochondrial phosphate transporter and methyltransferase genes contribute to fusarium head blight type ii disease resistance and grain development in wheat.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f9e7e7485b004b68bc09cabab4febf18
work_keys_str_mv	AT keshavbmalla mitochondrialphosphatetransporterandmethyltransferasegenescontributetofusariumheadblighttypeiidiseaseresistanceandgraindevelopmentinwheat AT ganeshthapa mitochondrialphosphatetransporterandmethyltransferasegenescontributetofusariumheadblighttypeiidiseaseresistanceandgraindevelopmentinwheat AT fionamdoohan mitochondrialphosphatetransporterandmethyltransferasegenescontributetofusariumheadblighttypeiidiseaseresistanceandgraindevelopmentinwheat
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Mitochondrial phosphate transporter and methyltransferase genes contribute to Fusarium head blight Type II disease resistance and grain development in wheat.

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