Fucoxanthin from microalgae Phaeodactylum tricornutum inhibits pro-inflammatory cytokines by regulating both NF-κB and NLRP3 inflammasome activation

Abstract Pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α are mediated by the activation of various kinds of signaling pathways in the innate immune system. Particularly, NF-κB and NLRP3 inflammasome signaling are involved in the production and secretion of these cytokines. Each signaling i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	A-Hyeon Lee, Hye-Yoon Shin, Jong-Hwi Park, Song Yi Koo, Sang Min Kim, Seung-Hoon Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9ea10da8d334362a17d0b165c4cb30e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f9ea10da8d334362a17d0b165c4cb30e
record_format	dspace
spelling	oai:doaj.org-article:f9ea10da8d334362a17d0b165c4cb30e2021-12-02T15:23:06ZFucoxanthin from microalgae Phaeodactylum tricornutum inhibits pro-inflammatory cytokines by regulating both NF-κB and NLRP3 inflammasome activation10.1038/s41598-020-80748-62045-2322https://doaj.org/article/f9ea10da8d334362a17d0b165c4cb30e2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80748-6https://doaj.org/toc/2045-2322Abstract Pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α are mediated by the activation of various kinds of signaling pathways in the innate immune system. Particularly, NF-κB and NLRP3 inflammasome signaling are involved in the production and secretion of these cytokines. Each signaling is participated in the two steps necessary for IL-1β, a representative pro-inflammatory cytokine, to be processed into a form secreted by cells. In the priming step stimulated by LPS, pro-IL-1β is synthesized through NF-κB activation. Pro-IL-1β cleavages into mature IL-1β by formed NLRP3 inflammasome in the activation step induced by ATP. The mature form of IL-1β is subsequently secreted out of the cell, causing inflammation. Moreover, IL-6 and TNF-α are known to increase in NLRP3 inflammasome-mediated conditions. Here, we found that fucoxanthin, one of the major components of Phaeodactylum tricornutum, has an inhibitory effect on NF-κB and NLRP3 inflammasome activation induced by the combination of LPS and ATP in bone marrow-derived immune cells as well as astrocytes. Fucoxanthin, which is abundant in the EtOH fraction of Phaeodactylum tricornutum extracts, has shown to have less cell toxicity and found to decrease the production of major pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α. Fucoxanthin has also shown to suppress the expression of cleaved caspase-1 and the oligomerization of ASC, which are the main components of the NLRP3 inflammasome. Furthermore, phosphorylated IκBα and pro-IL-1β expression decreased in the presence of fucoxanthin, suggesting that fucoxanthin can negatively regulate the priming step of inflammasome signaling. Thus, our results provide reliable evidence that fucoxanthin may serve as a key candidate in the development of potential therapeutic agents for inflammatory diseases as well as neurodegenerative diseases caused by NF-κB and NLRP3 inflammasome activation.A-Hyeon LeeHye-Yoon ShinJong-Hwi ParkSong Yi KooSang Min KimSeung-Hoon YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A-Hyeon Lee Hye-Yoon Shin Jong-Hwi Park Song Yi Koo Sang Min Kim Seung-Hoon Yang Fucoxanthin from microalgae Phaeodactylum tricornutum inhibits pro-inflammatory cytokines by regulating both NF-κB and NLRP3 inflammasome activation
description	Abstract Pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α are mediated by the activation of various kinds of signaling pathways in the innate immune system. Particularly, NF-κB and NLRP3 inflammasome signaling are involved in the production and secretion of these cytokines. Each signaling is participated in the two steps necessary for IL-1β, a representative pro-inflammatory cytokine, to be processed into a form secreted by cells. In the priming step stimulated by LPS, pro-IL-1β is synthesized through NF-κB activation. Pro-IL-1β cleavages into mature IL-1β by formed NLRP3 inflammasome in the activation step induced by ATP. The mature form of IL-1β is subsequently secreted out of the cell, causing inflammation. Moreover, IL-6 and TNF-α are known to increase in NLRP3 inflammasome-mediated conditions. Here, we found that fucoxanthin, one of the major components of Phaeodactylum tricornutum, has an inhibitory effect on NF-κB and NLRP3 inflammasome activation induced by the combination of LPS and ATP in bone marrow-derived immune cells as well as astrocytes. Fucoxanthin, which is abundant in the EtOH fraction of Phaeodactylum tricornutum extracts, has shown to have less cell toxicity and found to decrease the production of major pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α. Fucoxanthin has also shown to suppress the expression of cleaved caspase-1 and the oligomerization of ASC, which are the main components of the NLRP3 inflammasome. Furthermore, phosphorylated IκBα and pro-IL-1β expression decreased in the presence of fucoxanthin, suggesting that fucoxanthin can negatively regulate the priming step of inflammasome signaling. Thus, our results provide reliable evidence that fucoxanthin may serve as a key candidate in the development of potential therapeutic agents for inflammatory diseases as well as neurodegenerative diseases caused by NF-κB and NLRP3 inflammasome activation.
format	article
author	A-Hyeon Lee Hye-Yoon Shin Jong-Hwi Park Song Yi Koo Sang Min Kim Seung-Hoon Yang
author_facet	A-Hyeon Lee Hye-Yoon Shin Jong-Hwi Park Song Yi Koo Sang Min Kim Seung-Hoon Yang
author_sort	A-Hyeon Lee
title	Fucoxanthin from microalgae Phaeodactylum tricornutum inhibits pro-inflammatory cytokines by regulating both NF-κB and NLRP3 inflammasome activation
title_short	Fucoxanthin from microalgae Phaeodactylum tricornutum inhibits pro-inflammatory cytokines by regulating both NF-κB and NLRP3 inflammasome activation
title_full	Fucoxanthin from microalgae Phaeodactylum tricornutum inhibits pro-inflammatory cytokines by regulating both NF-κB and NLRP3 inflammasome activation
title_fullStr	Fucoxanthin from microalgae Phaeodactylum tricornutum inhibits pro-inflammatory cytokines by regulating both NF-κB and NLRP3 inflammasome activation
title_full_unstemmed	Fucoxanthin from microalgae Phaeodactylum tricornutum inhibits pro-inflammatory cytokines by regulating both NF-κB and NLRP3 inflammasome activation
title_sort	fucoxanthin from microalgae phaeodactylum tricornutum inhibits pro-inflammatory cytokines by regulating both nf-κb and nlrp3 inflammasome activation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f9ea10da8d334362a17d0b165c4cb30e
work_keys_str_mv	AT ahyeonlee fucoxanthinfrommicroalgaephaeodactylumtricornutuminhibitsproinflammatorycytokinesbyregulatingbothnfkbandnlrp3inflammasomeactivation AT hyeyoonshin fucoxanthinfrommicroalgaephaeodactylumtricornutuminhibitsproinflammatorycytokinesbyregulatingbothnfkbandnlrp3inflammasomeactivation AT jonghwipark fucoxanthinfrommicroalgaephaeodactylumtricornutuminhibitsproinflammatorycytokinesbyregulatingbothnfkbandnlrp3inflammasomeactivation AT songyikoo fucoxanthinfrommicroalgaephaeodactylumtricornutuminhibitsproinflammatorycytokinesbyregulatingbothnfkbandnlrp3inflammasomeactivation AT sangminkim fucoxanthinfrommicroalgaephaeodactylumtricornutuminhibitsproinflammatorycytokinesbyregulatingbothnfkbandnlrp3inflammasomeactivation AT seunghoonyang fucoxanthinfrommicroalgaephaeodactylumtricornutuminhibitsproinflammatorycytokinesbyregulatingbothnfkbandnlrp3inflammasomeactivation
_version_	1718387363998072832

Fucoxanthin from microalgae Phaeodactylum tricornutum inhibits pro-inflammatory cytokines by regulating both NF-κB and NLRP3 inflammasome activation

Ejemplares similares