A simple and fast non-radioactive bridging immunoassay for insulin autoantibodies.

Type 1 diabetes (T1D) is an autoimmune disease which results from the destruction of pancreatic beta cells. Autoantibodies directed against islet antigens are valuable diagnostic tools. Insulin autoantibodies (IAAs) are usually the first to appear and also the most difficult to detect amongst the fo...

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Autores principales:	Ingrid Kikkas, Roberto Mallone, Nadia Tubiana-Rufi, Didier Chevenne, Jean Claude Carel, Christophe Créminon, Hervé Volland, Christian Boitard, Nathalie Morel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9f5cf705ab049a4b911befdbe3068ac
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spelling	oai:doaj.org-article:f9f5cf705ab049a4b911befdbe3068ac2021-11-18T09:02:30ZA simple and fast non-radioactive bridging immunoassay for insulin autoantibodies.1932-620310.1371/journal.pone.0069021https://doaj.org/article/f9f5cf705ab049a4b911befdbe3068ac2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23922678/?tool=EBIhttps://doaj.org/toc/1932-6203Type 1 diabetes (T1D) is an autoimmune disease which results from the destruction of pancreatic beta cells. Autoantibodies directed against islet antigens are valuable diagnostic tools. Insulin autoantibodies (IAAs) are usually the first to appear and also the most difficult to detect amongst the four major islet autoantibodies. A non-radioactive IAA bridging ELISA was developed to this end. In this assay, one site of the IAAs from serum samples is bound to a hapten-labeled insulin (GC300-insulin), which is subsequently captured on anti-GC300 antibody-coated 96-well plates. The other site of the IAAs is bound to biotinylated insulin, allowing the complex to be detected by an enzyme-streptavidin conjugate. In the present study, 50 serum samples from patients with newly diagnosed T1D and 100 control sera from non-diabetic individuals were analyzed with our new assay and the results were correlated with an IAA radioimmunoassay (RIA). Using IAA bridging ELISA, IAAs were detected in 32 out of 50 T1D children, whereas with IAA RIA, 41 out of 50 children with newly diagnosed T1D were scored as positive. In conclusion, the IAA bridging ELISA could serve as an attractive approach for rapid and automated detection of IAAs in T1D patients for diagnostic purposes.Ingrid KikkasRoberto MalloneNadia Tubiana-RufiDidier ChevenneJean Claude CarelChristophe CréminonHervé VollandChristian BoitardNathalie MorelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69021 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ingrid Kikkas Roberto Mallone Nadia Tubiana-Rufi Didier Chevenne Jean Claude Carel Christophe Créminon Hervé Volland Christian Boitard Nathalie Morel A simple and fast non-radioactive bridging immunoassay for insulin autoantibodies.
description	Type 1 diabetes (T1D) is an autoimmune disease which results from the destruction of pancreatic beta cells. Autoantibodies directed against islet antigens are valuable diagnostic tools. Insulin autoantibodies (IAAs) are usually the first to appear and also the most difficult to detect amongst the four major islet autoantibodies. A non-radioactive IAA bridging ELISA was developed to this end. In this assay, one site of the IAAs from serum samples is bound to a hapten-labeled insulin (GC300-insulin), which is subsequently captured on anti-GC300 antibody-coated 96-well plates. The other site of the IAAs is bound to biotinylated insulin, allowing the complex to be detected by an enzyme-streptavidin conjugate. In the present study, 50 serum samples from patients with newly diagnosed T1D and 100 control sera from non-diabetic individuals were analyzed with our new assay and the results were correlated with an IAA radioimmunoassay (RIA). Using IAA bridging ELISA, IAAs were detected in 32 out of 50 T1D children, whereas with IAA RIA, 41 out of 50 children with newly diagnosed T1D were scored as positive. In conclusion, the IAA bridging ELISA could serve as an attractive approach for rapid and automated detection of IAAs in T1D patients for diagnostic purposes.
format	article
author	Ingrid Kikkas Roberto Mallone Nadia Tubiana-Rufi Didier Chevenne Jean Claude Carel Christophe Créminon Hervé Volland Christian Boitard Nathalie Morel
author_facet	Ingrid Kikkas Roberto Mallone Nadia Tubiana-Rufi Didier Chevenne Jean Claude Carel Christophe Créminon Hervé Volland Christian Boitard Nathalie Morel
author_sort	Ingrid Kikkas
title	A simple and fast non-radioactive bridging immunoassay for insulin autoantibodies.
title_short	A simple and fast non-radioactive bridging immunoassay for insulin autoantibodies.
title_full	A simple and fast non-radioactive bridging immunoassay for insulin autoantibodies.
title_fullStr	A simple and fast non-radioactive bridging immunoassay for insulin autoantibodies.
title_full_unstemmed	A simple and fast non-radioactive bridging immunoassay for insulin autoantibodies.
title_sort	simple and fast non-radioactive bridging immunoassay for insulin autoantibodies.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f9f5cf705ab049a4b911befdbe3068ac
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A simple and fast non-radioactive bridging immunoassay for insulin autoantibodies.

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