Severity of heterosubtypic influenza virus infection in ferrets is reduced by live attenuated influenza vaccine

Abstract Live attenuated influenza vaccine (LAIV) is widely used to protect humans from seasonal influenza infection, particularly in children. In contrast to inactivated vaccines, the LAIV can induce both mucosal and cellular immune responses. Here we show that a single dose of monovalent H1N1pdm09...

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Autores principales:	Anthony C. Marriott, Karen E. Gooch, Phillip J. Brown, Kathryn A. Ryan, Nicola J. Jones, Natasha Merredew, Nathan Wiblin, Oliver Dibben, Helen Bright, Bassam Hallis, Catherine J. Whittaker, Miles W. Carroll
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/f9f97dcbeef94e3ca43053085c7250dd
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spelling	oai:doaj.org-article:f9f97dcbeef94e3ca43053085c7250dd2021-12-02T14:24:55ZSeverity of heterosubtypic influenza virus infection in ferrets is reduced by live attenuated influenza vaccine10.1038/s41541-021-00306-72059-0105https://doaj.org/article/f9f97dcbeef94e3ca43053085c7250dd2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00306-7https://doaj.org/toc/2059-0105Abstract Live attenuated influenza vaccine (LAIV) is widely used to protect humans from seasonal influenza infection, particularly in children. In contrast to inactivated vaccines, the LAIV can induce both mucosal and cellular immune responses. Here we show that a single dose of monovalent H1N1pdm09-specific LAIV in the ferret model is fully protective against a subsequent wild-type H1N1pdm09 challenge, and furthermore reduces the severity of disease following challenge with a different influenza A subtype (H3N2). The reduced severity comprised reductions in weight loss and fever, as well as more rapid clearance of virus, compared to non-vaccinated H3N2-challenged ferrets. No H3N2-neutralizing antibodies were detected in vaccinated ferret sera. Rather, heterosubtypic protection correlated with interferon-gamma+ (IFN-γ+) T-cell responses measured in peripheral blood and in lung lymphocytes. The IFN-γ+ cells were cross-reactive to H3N2 virus even when obtained from vaccinated animals that had never been exposed to H3N2 virus. We believe this study provides compelling evidence that the LAIV can provide a significant reduction in infection and symptoms when challenged with heterosubtypic influenza strains not included in the LAIV, highlighting the importance of cross-reactive T-cells in the design of a universal influenza vaccine.Anthony C. MarriottKaren E. GoochPhillip J. BrownKathryn A. RyanNicola J. JonesNatasha MerredewNathan WiblinOliver DibbenHelen BrightBassam HallisCatherine J. WhittakerMiles W. CarrollNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Anthony C. Marriott Karen E. Gooch Phillip J. Brown Kathryn A. Ryan Nicola J. Jones Natasha Merredew Nathan Wiblin Oliver Dibben Helen Bright Bassam Hallis Catherine J. Whittaker Miles W. Carroll Severity of heterosubtypic influenza virus infection in ferrets is reduced by live attenuated influenza vaccine
description	Abstract Live attenuated influenza vaccine (LAIV) is widely used to protect humans from seasonal influenza infection, particularly in children. In contrast to inactivated vaccines, the LAIV can induce both mucosal and cellular immune responses. Here we show that a single dose of monovalent H1N1pdm09-specific LAIV in the ferret model is fully protective against a subsequent wild-type H1N1pdm09 challenge, and furthermore reduces the severity of disease following challenge with a different influenza A subtype (H3N2). The reduced severity comprised reductions in weight loss and fever, as well as more rapid clearance of virus, compared to non-vaccinated H3N2-challenged ferrets. No H3N2-neutralizing antibodies were detected in vaccinated ferret sera. Rather, heterosubtypic protection correlated with interferon-gamma+ (IFN-γ+) T-cell responses measured in peripheral blood and in lung lymphocytes. The IFN-γ+ cells were cross-reactive to H3N2 virus even when obtained from vaccinated animals that had never been exposed to H3N2 virus. We believe this study provides compelling evidence that the LAIV can provide a significant reduction in infection and symptoms when challenged with heterosubtypic influenza strains not included in the LAIV, highlighting the importance of cross-reactive T-cells in the design of a universal influenza vaccine.
format	article
author	Anthony C. Marriott Karen E. Gooch Phillip J. Brown Kathryn A. Ryan Nicola J. Jones Natasha Merredew Nathan Wiblin Oliver Dibben Helen Bright Bassam Hallis Catherine J. Whittaker Miles W. Carroll
author_facet	Anthony C. Marriott Karen E. Gooch Phillip J. Brown Kathryn A. Ryan Nicola J. Jones Natasha Merredew Nathan Wiblin Oliver Dibben Helen Bright Bassam Hallis Catherine J. Whittaker Miles W. Carroll
author_sort	Anthony C. Marriott
title	Severity of heterosubtypic influenza virus infection in ferrets is reduced by live attenuated influenza vaccine
title_short	Severity of heterosubtypic influenza virus infection in ferrets is reduced by live attenuated influenza vaccine
title_full	Severity of heterosubtypic influenza virus infection in ferrets is reduced by live attenuated influenza vaccine
title_fullStr	Severity of heterosubtypic influenza virus infection in ferrets is reduced by live attenuated influenza vaccine
title_full_unstemmed	Severity of heterosubtypic influenza virus infection in ferrets is reduced by live attenuated influenza vaccine
title_sort	severity of heterosubtypic influenza virus infection in ferrets is reduced by live attenuated influenza vaccine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f9f97dcbeef94e3ca43053085c7250dd
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Severity of heterosubtypic influenza virus infection in ferrets is reduced by live attenuated influenza vaccine

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