Topical Administration of Heat-Killed Enterococcus faecalis Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing

Lactic acid bacteria (LAB) are known to have beneficial effects on immune responses when they are orally administered as bacterial products. Although the beneficial effects of LAB have been reported for the genera Lactobacillus and Lactococcus, little has been u...

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Autores principales:	Hiromasa Tanno, Emi Kanno, Shiho Kurosaka, Yukari Oikawa, Takumi Watanabe, Ko Sato, Jun Kasamatsu, Tomomitsu Miyasaka, Shinyo Ishi, Miki Shoji, Naoyuki Takagi, Yoshimichi Imai, Keiko Ishii, Masahiro Tachi, Kazuyoshi Kawakami
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	wound-healing <i>Enterococcus faecalis</i> KH2 angiogenesis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f9fc564098254bf39ca3b5eabe7670e1
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spelling	oai:doaj.org-article:f9fc564098254bf39ca3b5eabe7670e12021-11-25T16:48:30ZTopical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing10.3390/biomedicines91115202227-9059https://doaj.org/article/f9fc564098254bf39ca3b5eabe7670e12021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1520https://doaj.org/toc/2227-9059Lactic acid bacteria (LAB) are known to have beneficial effects on immune responses when they are orally administered as bacterial products. Although the beneficial effects of LAB have been reported for the genera <i>Lactobacillus</i> and <i>Lactococcus</i>, little has been uncovered on the effects of the genus <i>Enterococcus</i> on skin wound-healing. In this study, we aimed to clarify the effect of heat-killed <i>Enterococcus faecalis</i> KH2 (heat-killed KH2) strain on the wound-healing process and to evaluate the therapeutic potential in chronic skin wounds. We analyzed percent wound closure, re-epithelialization, and granulation area, and cytokine and growth factor production. We found that heat-killed KH2 contributed to the acceleration of re-epithelialization and the formation of granulation tissue by inducing tumor necrosis factor-α, interleukin-6, basic fibroblast growth factor, transforming growth factor (TGF)-β1, and vascular endothelial growth factor production. In addition, heat-killed KH2 also improved wound closure, which was accompanied by the increased production of TGF-β1 in diabetic mice. Topical administration of heat-killed KH2 might have therapeutic potential for the treatment of chronic skin wounds in diabetes mellitus. In the present study, we concluded that heat-killed KH2 promoted skin wound-healing through the formation of granulation tissues and the production of inflammatory cytokines and growth factors.Hiromasa TannoEmi KannoShiho KurosakaYukari OikawaTakumi WatanabeKo SatoJun KasamatsuTomomitsu MiyasakaShinyo IshiMiki ShojiNaoyuki TakagiYoshimichi ImaiKeiko IshiiMasahiro TachiKazuyoshi KawakamiMDPI AGarticlewound-healing<i>Enterococcus faecalis</i> KH2angiogenesisBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1520, p 1520 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	wound-healing <i>Enterococcus faecalis</i> KH2 angiogenesis Biology (General) QH301-705.5
spellingShingle	wound-healing <i>Enterococcus faecalis</i> KH2 angiogenesis Biology (General) QH301-705.5 Hiromasa Tanno Emi Kanno Shiho Kurosaka Yukari Oikawa Takumi Watanabe Ko Sato Jun Kasamatsu Tomomitsu Miyasaka Shinyo Ishi Miki Shoji Naoyuki Takagi Yoshimichi Imai Keiko Ishii Masahiro Tachi Kazuyoshi Kawakami Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing
description	Lactic acid bacteria (LAB) are known to have beneficial effects on immune responses when they are orally administered as bacterial products. Although the beneficial effects of LAB have been reported for the genera <i>Lactobacillus</i> and <i>Lactococcus</i>, little has been uncovered on the effects of the genus <i>Enterococcus</i> on skin wound-healing. In this study, we aimed to clarify the effect of heat-killed <i>Enterococcus faecalis</i> KH2 (heat-killed KH2) strain on the wound-healing process and to evaluate the therapeutic potential in chronic skin wounds. We analyzed percent wound closure, re-epithelialization, and granulation area, and cytokine and growth factor production. We found that heat-killed KH2 contributed to the acceleration of re-epithelialization and the formation of granulation tissue by inducing tumor necrosis factor-α, interleukin-6, basic fibroblast growth factor, transforming growth factor (TGF)-β1, and vascular endothelial growth factor production. In addition, heat-killed KH2 also improved wound closure, which was accompanied by the increased production of TGF-β1 in diabetic mice. Topical administration of heat-killed KH2 might have therapeutic potential for the treatment of chronic skin wounds in diabetes mellitus. In the present study, we concluded that heat-killed KH2 promoted skin wound-healing through the formation of granulation tissues and the production of inflammatory cytokines and growth factors.
format	article
author	Hiromasa Tanno Emi Kanno Shiho Kurosaka Yukari Oikawa Takumi Watanabe Ko Sato Jun Kasamatsu Tomomitsu Miyasaka Shinyo Ishi Miki Shoji Naoyuki Takagi Yoshimichi Imai Keiko Ishii Masahiro Tachi Kazuyoshi Kawakami
author_facet	Hiromasa Tanno Emi Kanno Shiho Kurosaka Yukari Oikawa Takumi Watanabe Ko Sato Jun Kasamatsu Tomomitsu Miyasaka Shinyo Ishi Miki Shoji Naoyuki Takagi Yoshimichi Imai Keiko Ishii Masahiro Tachi Kazuyoshi Kawakami
author_sort	Hiromasa Tanno
title	Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing
title_short	Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing
title_full	Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing
title_fullStr	Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing
title_full_unstemmed	Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing
title_sort	topical administration of heat-killed <i>enterococcus faecalis</i> strain kh2 promotes re-epithelialization and granulation tissue formation during skin wound-healing
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f9fc564098254bf39ca3b5eabe7670e1
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Topical Administration of Heat-Killed <i>Enterococcus faecalis</i> Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing

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