Functional characterization of the PHT1 family transporters of foxtail millet with development of a novel Agrobacterium-mediated transformation procedure

Abstract Phosphate is an essential nutrient for plant growth and is acquired from the environment and distributed within the plant in part through the action of phosphate transporters of the PHT1 family. Foxtail millet (Setaria italica) is an orphan crop essential to the food security of many small...

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Autores principales:	S. Antony Ceasar, Alison Baker, S. Ignacimuthu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa00d99514ca42c5902ddcf7371e1df6
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spelling	oai:doaj.org-article:fa00d99514ca42c5902ddcf7371e1df62021-12-02T11:40:12ZFunctional characterization of the PHT1 family transporters of foxtail millet with development of a novel Agrobacterium-mediated transformation procedure10.1038/s41598-017-14447-02045-2322https://doaj.org/article/fa00d99514ca42c5902ddcf7371e1df62017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-14447-0https://doaj.org/toc/2045-2322Abstract Phosphate is an essential nutrient for plant growth and is acquired from the environment and distributed within the plant in part through the action of phosphate transporters of the PHT1 family. Foxtail millet (Setaria italica) is an orphan crop essential to the food security of many small farmers in Asia and Africa and is a model system for other millets. A novel Agrobacterium-mediated transformation and direct plant regeneration procedure was developed from shoot apex explants and used to downregulate expression of 3 members of the PHT1 phosphate transporter family SiPHT1;2 SiPHT1;3 and SiPHT1;4. Transformants were recovered with close to 10% efficiency. The downregulation of individual transporters was confirmed by RT-PCR. Downregulation of individual transporters significantly reduced the total and inorganic P contents in shoot and root tissues and increased the number of lateral roots and root hairs showing they have non-redundant roles. Downregulation of SiPHT1;2 had the strongest effect on total and inorganic P in shoot and root tissues. Complementation experiments in S. cerevisiae provide evidence for the ability of SiPHT1;1, 1;2, 1;3, 1;7 and 1;8 to function as high affinity Pi transporters. This work will aid development of improved millet varieties for global food security.S. Antony CeasarAlison BakerS. IgnacimuthuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S. Antony Ceasar Alison Baker S. Ignacimuthu Functional characterization of the PHT1 family transporters of foxtail millet with development of a novel Agrobacterium-mediated transformation procedure
description	Abstract Phosphate is an essential nutrient for plant growth and is acquired from the environment and distributed within the plant in part through the action of phosphate transporters of the PHT1 family. Foxtail millet (Setaria italica) is an orphan crop essential to the food security of many small farmers in Asia and Africa and is a model system for other millets. A novel Agrobacterium-mediated transformation and direct plant regeneration procedure was developed from shoot apex explants and used to downregulate expression of 3 members of the PHT1 phosphate transporter family SiPHT1;2 SiPHT1;3 and SiPHT1;4. Transformants were recovered with close to 10% efficiency. The downregulation of individual transporters was confirmed by RT-PCR. Downregulation of individual transporters significantly reduced the total and inorganic P contents in shoot and root tissues and increased the number of lateral roots and root hairs showing they have non-redundant roles. Downregulation of SiPHT1;2 had the strongest effect on total and inorganic P in shoot and root tissues. Complementation experiments in S. cerevisiae provide evidence for the ability of SiPHT1;1, 1;2, 1;3, 1;7 and 1;8 to function as high affinity Pi transporters. This work will aid development of improved millet varieties for global food security.
format	article
author	S. Antony Ceasar Alison Baker S. Ignacimuthu
author_facet	S. Antony Ceasar Alison Baker S. Ignacimuthu
author_sort	S. Antony Ceasar
title	Functional characterization of the PHT1 family transporters of foxtail millet with development of a novel Agrobacterium-mediated transformation procedure
title_short	Functional characterization of the PHT1 family transporters of foxtail millet with development of a novel Agrobacterium-mediated transformation procedure
title_full	Functional characterization of the PHT1 family transporters of foxtail millet with development of a novel Agrobacterium-mediated transformation procedure
title_fullStr	Functional characterization of the PHT1 family transporters of foxtail millet with development of a novel Agrobacterium-mediated transformation procedure
title_full_unstemmed	Functional characterization of the PHT1 family transporters of foxtail millet with development of a novel Agrobacterium-mediated transformation procedure
title_sort	functional characterization of the pht1 family transporters of foxtail millet with development of a novel agrobacterium-mediated transformation procedure
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fa00d99514ca42c5902ddcf7371e1df6
work_keys_str_mv	AT santonyceasar functionalcharacterizationofthepht1familytransportersoffoxtailmilletwithdevelopmentofanovelagrobacteriummediatedtransformationprocedure AT alisonbaker functionalcharacterizationofthepht1familytransportersoffoxtailmilletwithdevelopmentofanovelagrobacteriummediatedtransformationprocedure AT signacimuthu functionalcharacterizationofthepht1familytransportersoffoxtailmilletwithdevelopmentofanovelagrobacteriummediatedtransformationprocedure
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Functional characterization of the PHT1 family transporters of foxtail millet with development of a novel Agrobacterium-mediated transformation procedure

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