Kar9 symmetry breaking alone is insufficient to ensure spindle alignment

Abstract Spindle positioning must be tightly regulated to ensure asymmetric cell divisions are successful. In budding yeast, spindle positioning is mediated by the asymmetric localization of microtubule + end tracking protein Kar9. Kar9 asymmetry is believed to be essential for spindle alignment. Ho...

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Autores principales:	Miram Meziane, Rachel Genthial, Jackie Vogel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa04e7d4d13a495c8b0ef53736aaf624
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spelling	oai:doaj.org-article:fa04e7d4d13a495c8b0ef53736aaf6242021-12-02T12:11:52ZKar9 symmetry breaking alone is insufficient to ensure spindle alignment10.1038/s41598-021-83136-w2045-2322https://doaj.org/article/fa04e7d4d13a495c8b0ef53736aaf6242021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83136-whttps://doaj.org/toc/2045-2322Abstract Spindle positioning must be tightly regulated to ensure asymmetric cell divisions are successful. In budding yeast, spindle positioning is mediated by the asymmetric localization of microtubule + end tracking protein Kar9. Kar9 asymmetry is believed to be essential for spindle alignment. However, the temporal correlation between symmetry breaking and spindle alignment has not been measured. Here, we establish a method of quantifying Kar9 symmetry breaking and find that Kar9 asymmetry is not well coupled with stable spindle alignment. We report the spindles are not aligned in the majority of asymmetric cells. Rather, stable alignment is correlated with Kar9 residence in the bud, regardless of symmetry state. Our findings suggest that Kar9 asymmetry alone is insufficient for stable alignment and reveal a possible role for Swe1 in regulating Kar9 residence in the bud.Miram MezianeRachel GenthialJackie VogelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miram Meziane Rachel Genthial Jackie Vogel Kar9 symmetry breaking alone is insufficient to ensure spindle alignment
description	Abstract Spindle positioning must be tightly regulated to ensure asymmetric cell divisions are successful. In budding yeast, spindle positioning is mediated by the asymmetric localization of microtubule + end tracking protein Kar9. Kar9 asymmetry is believed to be essential for spindle alignment. However, the temporal correlation between symmetry breaking and spindle alignment has not been measured. Here, we establish a method of quantifying Kar9 symmetry breaking and find that Kar9 asymmetry is not well coupled with stable spindle alignment. We report the spindles are not aligned in the majority of asymmetric cells. Rather, stable alignment is correlated with Kar9 residence in the bud, regardless of symmetry state. Our findings suggest that Kar9 asymmetry alone is insufficient for stable alignment and reveal a possible role for Swe1 in regulating Kar9 residence in the bud.
format	article
author	Miram Meziane Rachel Genthial Jackie Vogel
author_facet	Miram Meziane Rachel Genthial Jackie Vogel
author_sort	Miram Meziane
title	Kar9 symmetry breaking alone is insufficient to ensure spindle alignment
title_short	Kar9 symmetry breaking alone is insufficient to ensure spindle alignment
title_full	Kar9 symmetry breaking alone is insufficient to ensure spindle alignment
title_fullStr	Kar9 symmetry breaking alone is insufficient to ensure spindle alignment
title_full_unstemmed	Kar9 symmetry breaking alone is insufficient to ensure spindle alignment
title_sort	kar9 symmetry breaking alone is insufficient to ensure spindle alignment
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fa04e7d4d13a495c8b0ef53736aaf624
work_keys_str_mv	AT mirammeziane kar9symmetrybreakingaloneisinsufficienttoensurespindlealignment AT rachelgenthial kar9symmetrybreakingaloneisinsufficienttoensurespindlealignment AT jackievogel kar9symmetrybreakingaloneisinsufficienttoensurespindlealignment
_version_	1718394609758896128

Kar9 symmetry breaking alone is insufficient to ensure spindle alignment

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