High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma

Background: Malignant fibrous histiocytoma (MFH) is a common type of soft tissue sarcoma and a serious threat to human health. MFH often relapses locally after the curettage is related to the residual cancer stem cells (CSCs). Currently, the dysregulation of microRNA (miRNA) has been found to be clo...

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Autores principales:	Dejian Li, Kai Zhao, Ziwen Zhao, Bo Jiang, Xianxu Gong, Yan Zhang, Yingqi Guo, Han Xiao, Ye Wang, Hui Liu, Chengqing Yi, Wenguang Gu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	miRNA malignant fibrous histiocytoma (MFH) cancer stem cell (CSC) ALDH+ cells ALDH– cells Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fa0aa6348ad84d44b779a74b05ee06eb
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spelling	oai:doaj.org-article:fa0aa6348ad84d44b779a74b05ee06eb2021-12-01T01:38:04ZHigh Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma2296-634X10.3389/fcell.2021.751833https://doaj.org/article/fa0aa6348ad84d44b779a74b05ee06eb2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.751833/fullhttps://doaj.org/toc/2296-634XBackground: Malignant fibrous histiocytoma (MFH) is a common type of soft tissue sarcoma and a serious threat to human health. MFH often relapses locally after the curettage is related to the residual cancer stem cells (CSCs). Currently, the dysregulation of microRNA (miRNA) has been found to be closely related to the recurrence of CSCs. However, whether dysregulations of miRNAs exist in MFH, CSCs remained unknown.Methods: In this study, miRNAs in MFH CSCs and MFH common cells were examined by gene probe. Then, target genes and their functions involved in the signal pathway were predicted by the relevant database. Finally, the miRNAs’ target regulatory network was constructed. Furthermore, the miRNAs and target genes were identified by quantitative polymerase chain reaction, whereas miRNA analogs and antagonists were transfected in tumor cells to investigate cell proliferation ability further.Results: Results showed that a total of 47 miRNAs were found, including 16 that were upregulated and 31 that were downregulated. The screened differential miRNA showed a different expression in the cell resistant strains compared with the control group. Quantitative polymerase chain reaction analysis confirmed that the relative abundance of seven miRNAs and four target genes varied significantly. The encouraging issue is that we found Hsa-miR-206 significantly inhibited MFH proliferative activity.Conclusion: Hsa-miR-206 played a key role in regulating MFH CSC properties that might be a representative marker and target for the diagnosis and treatment of MFH in the future.Dejian LiDejian LiKai ZhaoZiwen ZhaoBo JiangXianxu GongYan ZhangYingqi GuoHan XiaoYe WangHui LiuChengqing YiWenguang GuFrontiers Media S.A.articlemiRNAmalignant fibrous histiocytoma (MFH)cancer stem cell (CSC)ALDH+ cellsALDH– cellsBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	miRNA malignant fibrous histiocytoma (MFH) cancer stem cell (CSC) ALDH+ cells ALDH– cells Biology (General) QH301-705.5
spellingShingle	miRNA malignant fibrous histiocytoma (MFH) cancer stem cell (CSC) ALDH+ cells ALDH– cells Biology (General) QH301-705.5 Dejian Li Dejian Li Kai Zhao Ziwen Zhao Bo Jiang Xianxu Gong Yan Zhang Yingqi Guo Han Xiao Ye Wang Hui Liu Chengqing Yi Wenguang Gu High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma
description	Background: Malignant fibrous histiocytoma (MFH) is a common type of soft tissue sarcoma and a serious threat to human health. MFH often relapses locally after the curettage is related to the residual cancer stem cells (CSCs). Currently, the dysregulation of microRNA (miRNA) has been found to be closely related to the recurrence of CSCs. However, whether dysregulations of miRNAs exist in MFH, CSCs remained unknown.Methods: In this study, miRNAs in MFH CSCs and MFH common cells were examined by gene probe. Then, target genes and their functions involved in the signal pathway were predicted by the relevant database. Finally, the miRNAs’ target regulatory network was constructed. Furthermore, the miRNAs and target genes were identified by quantitative polymerase chain reaction, whereas miRNA analogs and antagonists were transfected in tumor cells to investigate cell proliferation ability further.Results: Results showed that a total of 47 miRNAs were found, including 16 that were upregulated and 31 that were downregulated. The screened differential miRNA showed a different expression in the cell resistant strains compared with the control group. Quantitative polymerase chain reaction analysis confirmed that the relative abundance of seven miRNAs and four target genes varied significantly. The encouraging issue is that we found Hsa-miR-206 significantly inhibited MFH proliferative activity.Conclusion: Hsa-miR-206 played a key role in regulating MFH CSC properties that might be a representative marker and target for the diagnosis and treatment of MFH in the future.
format	article
author	Dejian Li Dejian Li Kai Zhao Ziwen Zhao Bo Jiang Xianxu Gong Yan Zhang Yingqi Guo Han Xiao Ye Wang Hui Liu Chengqing Yi Wenguang Gu
author_facet	Dejian Li Dejian Li Kai Zhao Ziwen Zhao Bo Jiang Xianxu Gong Yan Zhang Yingqi Guo Han Xiao Ye Wang Hui Liu Chengqing Yi Wenguang Gu
author_sort	Dejian Li
title	High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma
title_short	High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma
title_full	High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma
title_fullStr	High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma
title_full_unstemmed	High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma
title_sort	high expression microrna-206 inhibits the growth of tumor cells in human malignant fibrous histiocytoma
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/fa0aa6348ad84d44b779a74b05ee06eb
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High Expression MicroRNA-206 Inhibits the Growth of Tumor Cells in Human Malignant Fibrous Histiocytoma

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