Characterising clinical Staphylococcus aureus isolates from the sinuses of patients with chronic rhinosinusitis

Abstract The role of Staphylococcus aureus in the pathogenesis of the chronic sinonasal disease chronic rhinosinusitis (CRS), has not been definitively established. Comparative analyses of S. aureus isolates from CRS with those from control participants may offer insight into a possible pathogenic l...

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Autores principales:	Brett Wagner Mackenzie, Melissa Zoing, Fiona Clow, David W. Waite, Fiona J. Radcliff, Michael W. Taylor, Kristi Biswas, Richard G. Douglas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa0bbe6bbdc04bf4a4adf456bbc8008c
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spelling	oai:doaj.org-article:fa0bbe6bbdc04bf4a4adf456bbc8008c2021-11-14T12:19:25ZCharacterising clinical Staphylococcus aureus isolates from the sinuses of patients with chronic rhinosinusitis10.1038/s41598-021-01297-02045-2322https://doaj.org/article/fa0bbe6bbdc04bf4a4adf456bbc8008c2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01297-0https://doaj.org/toc/2045-2322Abstract The role of Staphylococcus aureus in the pathogenesis of the chronic sinonasal disease chronic rhinosinusitis (CRS), has not been definitively established. Comparative analyses of S. aureus isolates from CRS with those from control participants may offer insight into a possible pathogenic link between this organism and CRS. The intra- and inter-subject S. aureus strain-level diversity in the sinuses of patients with and without CRS were compared in this cross-sectional study. In total, 100 patients (CRS = 64, control = 36) were screened for S. aureus carriage. The overall carriage prevalence of S. aureus in this cohort was 24% (CRS n = 13, control n = 11). Cultured S. aureus isolates from 18 participants were strain-typed using spa gene sequencing. The bacterial community composition of the middle meatus was assessed using amplicon sequencing targeting the V3V4 hypervariable region of the bacterial 16S rRNA gene. S. aureus isolates cultured from patients were grown in co-culture with the commensal bacterium Dolosigranulum pigrum and characterised. All participants harboured a single S. aureus strain and no trend in disease-specific strain-level diversity was observed. Bacterial community analyses revealed a significant negative correlation in the relative abundances of S. aureus and D. pigrum sequences, suggesting an antagonistic interaction between these organisms. Co-cultivation experiments with these bacteria, however, did not confirm this interaction in vitro. We saw no significant associations of CRS disease with S. aureus strain types. The functional role that S. aureus occupies in CRS likely depends on other factors such as variations in gene expression and interactions with other members of the sinus bacterial community.Brett Wagner MackenzieMelissa ZoingFiona ClowDavid W. WaiteFiona J. RadcliffMichael W. TaylorKristi BiswasRichard G. DouglasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Brett Wagner Mackenzie Melissa Zoing Fiona Clow David W. Waite Fiona J. Radcliff Michael W. Taylor Kristi Biswas Richard G. Douglas Characterising clinical Staphylococcus aureus isolates from the sinuses of patients with chronic rhinosinusitis
description	Abstract The role of Staphylococcus aureus in the pathogenesis of the chronic sinonasal disease chronic rhinosinusitis (CRS), has not been definitively established. Comparative analyses of S. aureus isolates from CRS with those from control participants may offer insight into a possible pathogenic link between this organism and CRS. The intra- and inter-subject S. aureus strain-level diversity in the sinuses of patients with and without CRS were compared in this cross-sectional study. In total, 100 patients (CRS = 64, control = 36) were screened for S. aureus carriage. The overall carriage prevalence of S. aureus in this cohort was 24% (CRS n = 13, control n = 11). Cultured S. aureus isolates from 18 participants were strain-typed using spa gene sequencing. The bacterial community composition of the middle meatus was assessed using amplicon sequencing targeting the V3V4 hypervariable region of the bacterial 16S rRNA gene. S. aureus isolates cultured from patients were grown in co-culture with the commensal bacterium Dolosigranulum pigrum and characterised. All participants harboured a single S. aureus strain and no trend in disease-specific strain-level diversity was observed. Bacterial community analyses revealed a significant negative correlation in the relative abundances of S. aureus and D. pigrum sequences, suggesting an antagonistic interaction between these organisms. Co-cultivation experiments with these bacteria, however, did not confirm this interaction in vitro. We saw no significant associations of CRS disease with S. aureus strain types. The functional role that S. aureus occupies in CRS likely depends on other factors such as variations in gene expression and interactions with other members of the sinus bacterial community.
format	article
author	Brett Wagner Mackenzie Melissa Zoing Fiona Clow David W. Waite Fiona J. Radcliff Michael W. Taylor Kristi Biswas Richard G. Douglas
author_facet	Brett Wagner Mackenzie Melissa Zoing Fiona Clow David W. Waite Fiona J. Radcliff Michael W. Taylor Kristi Biswas Richard G. Douglas
author_sort	Brett Wagner Mackenzie
title	Characterising clinical Staphylococcus aureus isolates from the sinuses of patients with chronic rhinosinusitis
title_short	Characterising clinical Staphylococcus aureus isolates from the sinuses of patients with chronic rhinosinusitis
title_full	Characterising clinical Staphylococcus aureus isolates from the sinuses of patients with chronic rhinosinusitis
title_fullStr	Characterising clinical Staphylococcus aureus isolates from the sinuses of patients with chronic rhinosinusitis
title_full_unstemmed	Characterising clinical Staphylococcus aureus isolates from the sinuses of patients with chronic rhinosinusitis
title_sort	characterising clinical staphylococcus aureus isolates from the sinuses of patients with chronic rhinosinusitis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fa0bbe6bbdc04bf4a4adf456bbc8008c
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Characterising clinical Staphylococcus aureus isolates from the sinuses of patients with chronic rhinosinusitis

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