Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (Solanum lycopersicum) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics

The tomato is a research model for fruit-ripening, however, its fruit-ripening mechanism still needs more extensive and in-depth exploration. Here, using TMT and LC-MS, the proteome and phosphoproteome of AC++ (wild type) and rin (ripening-inhibitor) mutant...

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Autores principales:	Qiaoli Xie, Yanling Tian, Zongli Hu, Lincheng Zhang, Boyan Tang, Yunshu Wang, Jing Li, Guoping Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	proteomics phosphoproteomics fruit-ripening <i>rin</i> tomato (<i>Solanum lycopersicum</i>) Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/fa15d337f28e4e17ab02da7e281d22a2
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spelling	oai:doaj.org-article:fa15d337f28e4e17ab02da7e281d22a22021-11-11T17:14:04ZNovel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics10.3390/ijms2221117821422-00671661-6596https://doaj.org/article/fa15d337f28e4e17ab02da7e281d22a22021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11782https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The tomato is a research model for fruit-ripening, however, its fruit-ripening mechanism still needs more extensive and in-depth exploration. Here, using TMT and LC-MS, the proteome and phosphoproteome of AC<sup>++</sup> (wild type) and <i>rin</i> (ripening-inhibitor) mutant fruits were studied to investigate the translation and post-translational regulation mechanisms of tomato fruit-ripening. A total of 6141 proteins and 4011 phosphorylation sites contained quantitative information. One-hundred proteins were identified in both omics’ profiles, which were mainly found in ethylene biosynthesis and signal transduction, photosynthesis regulation, carotenoid and flavonoid biosynthesis, chlorophyll degradation, ribosomal subunit expression changes, MAPK pathway, transcription factors and kinases. The affected protein levels were correlated with their corresponding gene transcript levels, such as NAC-NOR, MADS-RIN, IMA, TAGL1, MADS-MC and TDR4. Changes in the phosphorylation levels of NAC-NOR and IMA were involved in the regulation of tomato fruit-ripening. Although photosynthesis was inhibited, there were diverse primary and secondary metabolic pathways, such as glycolysis, fatty acid metabolism, vitamin metabolism and isoprenoid biosynthesis, regulated by phosphorylation. These data constitute a map of protein—protein phosphorylation in the regulation of tomato fruit-ripening, which lays the foundation for future in-depth study of the sophisticated molecular mechanisms of fruit-ripening and provide guidance for molecular breeding.Qiaoli XieYanling TianZongli HuLincheng ZhangBoyan TangYunshu WangJing LiGuoping ChenMDPI AGarticleproteomicsphosphoproteomicsfruit-ripening<i>rin</i>tomato (<i>Solanum lycopersicum</i>)Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11782, p 11782 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	proteomics phosphoproteomics fruit-ripening <i>rin</i> tomato (<i>Solanum lycopersicum</i>) Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	proteomics phosphoproteomics fruit-ripening <i>rin</i> tomato (<i>Solanum lycopersicum</i>) Biology (General) QH301-705.5 Chemistry QD1-999 Qiaoli Xie Yanling Tian Zongli Hu Lincheng Zhang Boyan Tang Yunshu Wang Jing Li Guoping Chen Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics
description	The tomato is a research model for fruit-ripening, however, its fruit-ripening mechanism still needs more extensive and in-depth exploration. Here, using TMT and LC-MS, the proteome and phosphoproteome of AC<sup>++</sup> (wild type) and <i>rin</i> (ripening-inhibitor) mutant fruits were studied to investigate the translation and post-translational regulation mechanisms of tomato fruit-ripening. A total of 6141 proteins and 4011 phosphorylation sites contained quantitative information. One-hundred proteins were identified in both omics’ profiles, which were mainly found in ethylene biosynthesis and signal transduction, photosynthesis regulation, carotenoid and flavonoid biosynthesis, chlorophyll degradation, ribosomal subunit expression changes, MAPK pathway, transcription factors and kinases. The affected protein levels were correlated with their corresponding gene transcript levels, such as NAC-NOR, MADS-RIN, IMA, TAGL1, MADS-MC and TDR4. Changes in the phosphorylation levels of NAC-NOR and IMA were involved in the regulation of tomato fruit-ripening. Although photosynthesis was inhibited, there were diverse primary and secondary metabolic pathways, such as glycolysis, fatty acid metabolism, vitamin metabolism and isoprenoid biosynthesis, regulated by phosphorylation. These data constitute a map of protein—protein phosphorylation in the regulation of tomato fruit-ripening, which lays the foundation for future in-depth study of the sophisticated molecular mechanisms of fruit-ripening and provide guidance for molecular breeding.
format	article
author	Qiaoli Xie Yanling Tian Zongli Hu Lincheng Zhang Boyan Tang Yunshu Wang Jing Li Guoping Chen
author_facet	Qiaoli Xie Yanling Tian Zongli Hu Lincheng Zhang Boyan Tang Yunshu Wang Jing Li Guoping Chen
author_sort	Qiaoli Xie
title	Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics
title_short	Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics
title_full	Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics
title_fullStr	Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics
title_full_unstemmed	Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics
title_sort	novel translational and phosphorylation modification regulation mechanisms of tomato (<i>solanum lycopersicum</i>) fruit ripening revealed by integrative proteomics and phosphoproteomics
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fa15d337f28e4e17ab02da7e281d22a2
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Novel Translational and Phosphorylation Modification Regulation Mechanisms of Tomato (<i>Solanum lycopersicum</i>) Fruit Ripening Revealed by Integrative Proteomics and Phosphoproteomics

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