Identification of essential genes for Escherichia coli aryl polyene biosynthesis and function in biofilm formation

Abstract Aryl polyenes (APEs) are specialized polyunsaturated carboxylic acids that were identified in silico as the product of the most widespread family of bacterial biosynthetic gene clusters (BGCs). They are present in several Gram-negative host-associated bacteria, including multidrug-resistant...

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Autores principales:	Isabel Johnston, Lucas J. Osborn, Rachel L. Markley, Elizabeth A. McManus, Anagha Kadam, Karlee B. Schultz, Nagashreyaa Nagajothi, Philip P. Ahern, J. Mark Brown, Jan Claesen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/fa1a661b9c6b49bfbb6551d03da69c3f
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spelling	oai:doaj.org-article:fa1a661b9c6b49bfbb6551d03da69c3f2021-12-02T16:10:46ZIdentification of essential genes for Escherichia coli aryl polyene biosynthesis and function in biofilm formation10.1038/s41522-021-00226-32055-5008https://doaj.org/article/fa1a661b9c6b49bfbb6551d03da69c3f2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00226-3https://doaj.org/toc/2055-5008Abstract Aryl polyenes (APEs) are specialized polyunsaturated carboxylic acids that were identified in silico as the product of the most widespread family of bacterial biosynthetic gene clusters (BGCs). They are present in several Gram-negative host-associated bacteria, including multidrug-resistant human pathogens. Here, we characterize a biological function of APEs, focusing on the BGC from a uropathogenic Escherichia coli (UPEC) strain. We first perform a genetic deletion analysis to identify the essential genes required for APE biosynthesis. Next, we show that APEs function as fitness factors that increase protection from oxidative stress and contribute to biofilm formation. Together, our study highlights key steps in the APE biosynthesis pathway that can be explored as potential drug targets for complementary strategies to reduce fitness and prevent biofilm formation of multi-drug resistant pathogens.Isabel JohnstonLucas J. OsbornRachel L. MarkleyElizabeth A. McManusAnagha KadamKarlee B. SchultzNagashreyaa NagajothiPhilip P. AhernJ. Mark BrownJan ClaesenNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Isabel Johnston Lucas J. Osborn Rachel L. Markley Elizabeth A. McManus Anagha Kadam Karlee B. Schultz Nagashreyaa Nagajothi Philip P. Ahern J. Mark Brown Jan Claesen Identification of essential genes for Escherichia coli aryl polyene biosynthesis and function in biofilm formation
description	Abstract Aryl polyenes (APEs) are specialized polyunsaturated carboxylic acids that were identified in silico as the product of the most widespread family of bacterial biosynthetic gene clusters (BGCs). They are present in several Gram-negative host-associated bacteria, including multidrug-resistant human pathogens. Here, we characterize a biological function of APEs, focusing on the BGC from a uropathogenic Escherichia coli (UPEC) strain. We first perform a genetic deletion analysis to identify the essential genes required for APE biosynthesis. Next, we show that APEs function as fitness factors that increase protection from oxidative stress and contribute to biofilm formation. Together, our study highlights key steps in the APE biosynthesis pathway that can be explored as potential drug targets for complementary strategies to reduce fitness and prevent biofilm formation of multi-drug resistant pathogens.
format	article
author	Isabel Johnston Lucas J. Osborn Rachel L. Markley Elizabeth A. McManus Anagha Kadam Karlee B. Schultz Nagashreyaa Nagajothi Philip P. Ahern J. Mark Brown Jan Claesen
author_facet	Isabel Johnston Lucas J. Osborn Rachel L. Markley Elizabeth A. McManus Anagha Kadam Karlee B. Schultz Nagashreyaa Nagajothi Philip P. Ahern J. Mark Brown Jan Claesen
author_sort	Isabel Johnston
title	Identification of essential genes for Escherichia coli aryl polyene biosynthesis and function in biofilm formation
title_short	Identification of essential genes for Escherichia coli aryl polyene biosynthesis and function in biofilm formation
title_full	Identification of essential genes for Escherichia coli aryl polyene biosynthesis and function in biofilm formation
title_fullStr	Identification of essential genes for Escherichia coli aryl polyene biosynthesis and function in biofilm formation
title_full_unstemmed	Identification of essential genes for Escherichia coli aryl polyene biosynthesis and function in biofilm formation
title_sort	identification of essential genes for escherichia coli aryl polyene biosynthesis and function in biofilm formation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fa1a661b9c6b49bfbb6551d03da69c3f
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Identification of essential genes for Escherichia coli aryl polyene biosynthesis and function in biofilm formation

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