Avian adeno-associated virus vector efficiently transduces neurons in the embryonic and post-embryonic chicken brain.

The domestic chicken is an attractive model system to explore the development and function of brain circuits. Electroporation-mediated and retrovirus (including lentivirus) vector-mediated gene transfer techniques have been widely used to introduce genetic material into chicken cells. However, it is...

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Autores principales:	Ryosuke Matsui, Yasuto Tanabe, Dai Watanabe
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa234d0e442f4e35a4eebab530affdfc
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spelling	oai:doaj.org-article:fa234d0e442f4e35a4eebab530affdfc2021-11-18T08:09:42ZAvian adeno-associated virus vector efficiently transduces neurons in the embryonic and post-embryonic chicken brain.1932-620310.1371/journal.pone.0048730https://doaj.org/article/fa234d0e442f4e35a4eebab530affdfc2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23144948/?tool=EBIhttps://doaj.org/toc/1932-6203The domestic chicken is an attractive model system to explore the development and function of brain circuits. Electroporation-mediated and retrovirus (including lentivirus) vector-mediated gene transfer techniques have been widely used to introduce genetic material into chicken cells. However, it is still challenging to efficiently transduce chicken postmitotic neurons without harming the cells. To overcome this problem, we searched for a virus vector suitable for gene transfer into chicken neurons, and report here a novel recombinant virus vector derived from avian adeno-associated virus (A3V). A3V vector efficiently transduces neuronal cells, but not non-neuronal cells in the brain. A single A3V injection into a postembryonic chick brain allows gene expression selectively in neuronal cells within 24 hrs. Such rapid and neuron-specific gene transduction raises the possibility that A3V vector can be utilized for studies of memory formation in filial imprinting, which occurs during the early postnatal days. A3V injection into the neural tube near the ear vesicle at early embryonic stage resulted in persistent and robust gene expression until E20.5 in the auditory brainstem. We further devised an A3V-mediated tetracycline (Tet) dependent gene expression system as a tool for studying the auditory circuit, consisting of the nucleus magnocellularis (NM) and nucleus laminaris (NL), that primarily computes interaural time differences (ITDs). Using this Tet system, we can transduce NM neurons without affecting NL neurons. Thus, the A3V technology complements current gene transfer techniques in chicken studies and will contribute to better understanding of the functional organization of neural circuits.Ryosuke MatsuiYasuto TanabeDai WatanabePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e48730 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryosuke Matsui Yasuto Tanabe Dai Watanabe Avian adeno-associated virus vector efficiently transduces neurons in the embryonic and post-embryonic chicken brain.
description	The domestic chicken is an attractive model system to explore the development and function of brain circuits. Electroporation-mediated and retrovirus (including lentivirus) vector-mediated gene transfer techniques have been widely used to introduce genetic material into chicken cells. However, it is still challenging to efficiently transduce chicken postmitotic neurons without harming the cells. To overcome this problem, we searched for a virus vector suitable for gene transfer into chicken neurons, and report here a novel recombinant virus vector derived from avian adeno-associated virus (A3V). A3V vector efficiently transduces neuronal cells, but not non-neuronal cells in the brain. A single A3V injection into a postembryonic chick brain allows gene expression selectively in neuronal cells within 24 hrs. Such rapid and neuron-specific gene transduction raises the possibility that A3V vector can be utilized for studies of memory formation in filial imprinting, which occurs during the early postnatal days. A3V injection into the neural tube near the ear vesicle at early embryonic stage resulted in persistent and robust gene expression until E20.5 in the auditory brainstem. We further devised an A3V-mediated tetracycline (Tet) dependent gene expression system as a tool for studying the auditory circuit, consisting of the nucleus magnocellularis (NM) and nucleus laminaris (NL), that primarily computes interaural time differences (ITDs). Using this Tet system, we can transduce NM neurons without affecting NL neurons. Thus, the A3V technology complements current gene transfer techniques in chicken studies and will contribute to better understanding of the functional organization of neural circuits.
format	article
author	Ryosuke Matsui Yasuto Tanabe Dai Watanabe
author_facet	Ryosuke Matsui Yasuto Tanabe Dai Watanabe
author_sort	Ryosuke Matsui
title	Avian adeno-associated virus vector efficiently transduces neurons in the embryonic and post-embryonic chicken brain.
title_short	Avian adeno-associated virus vector efficiently transduces neurons in the embryonic and post-embryonic chicken brain.
title_full	Avian adeno-associated virus vector efficiently transduces neurons in the embryonic and post-embryonic chicken brain.
title_fullStr	Avian adeno-associated virus vector efficiently transduces neurons in the embryonic and post-embryonic chicken brain.
title_full_unstemmed	Avian adeno-associated virus vector efficiently transduces neurons in the embryonic and post-embryonic chicken brain.
title_sort	avian adeno-associated virus vector efficiently transduces neurons in the embryonic and post-embryonic chicken brain.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/fa234d0e442f4e35a4eebab530affdfc
work_keys_str_mv	AT ryosukematsui avianadenoassociatedvirusvectorefficientlytransducesneuronsintheembryonicandpostembryonicchickenbrain AT yasutotanabe avianadenoassociatedvirusvectorefficientlytransducesneuronsintheembryonicandpostembryonicchickenbrain AT daiwatanabe avianadenoassociatedvirusvectorefficientlytransducesneuronsintheembryonicandpostembryonicchickenbrain
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Avian adeno-associated virus vector efficiently transduces neurons in the embryonic and post-embryonic chicken brain.

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