Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica

Abstract Methyl erythritol phosphate (MEP) is the metabolite found in the MEP pathway for isoprenoid biosynthesis, which is known to be utilized by plants, algae, and bacteria. In this study, an unprecedented observation was found in the oleaginous yeast Yarrowia lipolytica, in which one of the chro...

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Autores principales:	Sivamoke Dissook, Tomohisa Kuzuyama, Yuri Nishimoto, Shigeru Kitani, Sastia Putri, Eiichiro Fukusaki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa24a3f4c39f48d4927044ce076bac47
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spelling	oai:doaj.org-article:fa24a3f4c39f48d4927044ce076bac472021-12-02T11:35:52ZStable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica10.1038/s41598-021-85170-02045-2322https://doaj.org/article/fa24a3f4c39f48d4927044ce076bac472021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85170-0https://doaj.org/toc/2045-2322Abstract Methyl erythritol phosphate (MEP) is the metabolite found in the MEP pathway for isoprenoid biosynthesis, which is known to be utilized by plants, algae, and bacteria. In this study, an unprecedented observation was found in the oleaginous yeast Yarrowia lipolytica, in which one of the chromatographic peaks was annotated as MEP when cultivated in the nitrogen limiting condition. This finding raised an interesting hypothesis of whether Y. lipolytica utilizes the MEP pathway for isoprenoid biosynthesis or not, because there is no report of yeast harboring the MEP pathway. Three independent approaches were used to investigate the existence of the MEP pathway in Y. lipolytica; the spiking of the authentic standard, the MEP pathway inhibitor, and the 13C labeling incorporation analysis. The study suggested that the mevalonate and MEP pathways co-exist in Y. lipolytica and the nitrogen limiting condition triggers the utilization of the MEP pathway in Y. lipolytica.Sivamoke DissookTomohisa KuzuyamaYuri NishimotoShigeru KitaniSastia PutriEiichiro FukusakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sivamoke Dissook Tomohisa Kuzuyama Yuri Nishimoto Shigeru Kitani Sastia Putri Eiichiro Fukusaki Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica
description	Abstract Methyl erythritol phosphate (MEP) is the metabolite found in the MEP pathway for isoprenoid biosynthesis, which is known to be utilized by plants, algae, and bacteria. In this study, an unprecedented observation was found in the oleaginous yeast Yarrowia lipolytica, in which one of the chromatographic peaks was annotated as MEP when cultivated in the nitrogen limiting condition. This finding raised an interesting hypothesis of whether Y. lipolytica utilizes the MEP pathway for isoprenoid biosynthesis or not, because there is no report of yeast harboring the MEP pathway. Three independent approaches were used to investigate the existence of the MEP pathway in Y. lipolytica; the spiking of the authentic standard, the MEP pathway inhibitor, and the 13C labeling incorporation analysis. The study suggested that the mevalonate and MEP pathways co-exist in Y. lipolytica and the nitrogen limiting condition triggers the utilization of the MEP pathway in Y. lipolytica.
format	article
author	Sivamoke Dissook Tomohisa Kuzuyama Yuri Nishimoto Shigeru Kitani Sastia Putri Eiichiro Fukusaki
author_facet	Sivamoke Dissook Tomohisa Kuzuyama Yuri Nishimoto Shigeru Kitani Sastia Putri Eiichiro Fukusaki
author_sort	Sivamoke Dissook
title	Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica
title_short	Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica
title_full	Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica
title_fullStr	Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica
title_full_unstemmed	Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica
title_sort	stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast yarrowia lipolytica
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fa24a3f4c39f48d4927044ce076bac47
work_keys_str_mv	AT sivamokedissook stableisotopeandchemicalinhibitionanalysessuggestedtheexistenceofanonmevalonatelikepathwayintheyeastyarrowialipolytica AT tomohisakuzuyama stableisotopeandchemicalinhibitionanalysessuggestedtheexistenceofanonmevalonatelikepathwayintheyeastyarrowialipolytica AT yurinishimoto stableisotopeandchemicalinhibitionanalysessuggestedtheexistenceofanonmevalonatelikepathwayintheyeastyarrowialipolytica AT shigerukitani stableisotopeandchemicalinhibitionanalysessuggestedtheexistenceofanonmevalonatelikepathwayintheyeastyarrowialipolytica AT sastiaputri stableisotopeandchemicalinhibitionanalysessuggestedtheexistenceofanonmevalonatelikepathwayintheyeastyarrowialipolytica AT eiichirofukusaki stableisotopeandchemicalinhibitionanalysessuggestedtheexistenceofanonmevalonatelikepathwayintheyeastyarrowialipolytica
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Stable isotope and chemical inhibition analyses suggested the existence of a non-mevalonate-like pathway in the yeast Yarrowia lipolytica

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