Improved drug loading and antibacterial activity of minocycline-loaded PLGA nanoparticles prepared by solid/oil/water ion pairing method

Tahereh Sadat Jafarzadeh Kashi1, Solmaz Eskandarion1,2, Mehdi Esfandyari-Manesh3,4, Seyyed Mahmoud Amin Marashi5, Nasrin Samadi6, Seyyed Mostafa Fatemi1,7, Fatemeh Atyabi2,3, Saeed Eshraghi8, Rassoul Dinarvand2,31Dental Materials Department, Faculty of Dentistry, 2Department of Pharmaceutics, 3Nanot...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jafarzadeh Kashi TS, Eskandarion S, Esfandyari-Manesh M, Amin Marashi SM, Samadi N, Fatemi SM, Atyabi F, Eshraghi S, Dinarv, R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fa264121f9654c71a6513191a2075bd2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fa264121f9654c71a6513191a2075bd2
record_format	dspace
spelling	oai:doaj.org-article:fa264121f9654c71a6513191a2075bd22021-12-02T02:34:26ZImproved drug loading and antibacterial activity of minocycline-loaded PLGA nanoparticles prepared by solid/oil/water ion pairing method1176-91141178-2013https://doaj.org/article/fa264121f9654c71a6513191a2075bd22012-01-01T00:00:00Zhttp://www.dovepress.com/improved-drug-loading-and-antibacterial-activity-of-minocycline-loaded-a9032https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Tahereh Sadat Jafarzadeh Kashi1, Solmaz Eskandarion1,2, Mehdi Esfandyari-Manesh3,4, Seyyed Mahmoud Amin Marashi5, Nasrin Samadi6, Seyyed Mostafa Fatemi1,7, Fatemeh Atyabi2,3, Saeed Eshraghi8, Rassoul Dinarvand2,31Dental Materials Department, Faculty of Dentistry, 2Department of Pharmaceutics, 3Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 4Department of Chemistry, Amirkabir University of Technology, Tehran, 5Department of Microbiology and Immunology, Faculty of Medicine, Babol University of Medical Sciences, Babol, 6Drug and Food Control Department, Faculty of Pharmacy, 7Research Center of Science and Technology in Medicine, 8Department of Microbiology, Tehran University of Medical Sciences, Tehran, IranBackground: Low drug entrapment efficiency of hydrophilic drugs into poly(lactic-co-glycolic acid) (PLGA) nanoparticles is a major drawback. The objective of this work was to investigate different methods of producing PLGA nanoparticles containing minocycline, a drug suitable for periodontal infections.Methods: Different methods, such as single and double solvent evaporation emulsion, ion pairing, and nanoprecipitation were used to prepare both PLGA and PEGylated PLGA nanoparticles. The resulting nanoparticles were analyzed for their morphology, particle size and size distribution, drug loading and entrapment efficiency, thermal properties, and antibacterial activity.Results: The nanoparticles prepared in this study were spherical, with an average particle size of 85&ndash;424 nm. The entrapment efficiency of the nanoparticles prepared using different methods was as follows: solid/oil/water ion pairing (29.9%) &gt; oil/oil (5.5%) &gt; water/oil/water (4.7%) &gt; modified oil/water (4.1%) &gt; nano precipitation (0.8%). Addition of dextran sulfate as an ion pairing agent, acting as an ionic spacer between PEGylated PLGA and minocycline, decreased the water solubility of minocycline, hence increasing the drug entrapment efficiency. Entrapment efficiency was also increased when low molecular weight PLGA and high molecular weight dextran sulfate was used. Drug release studies performed in phosphate buffer at pH 7.4 indicated slow release of minocycline from 3 days to several weeks. On antibacterial analysis, the minimum inhibitory concentration and minimum bactericidal concentration of nanoparticles was at least two times lower than that of the free drug.Conclusion: Novel minocycline-PEGylated PLGA nanoparticles prepared by the ion pairing method had the best drug loading and entrapment efficiency compared with other prepared nanoparticles. They also showed higher in vitro antibacterial activity than the free drug.Keywords: nanoparticle, PEGylation, PLGA, ion pairing, minocycline, antibacterialJafarzadeh Kashi TSEskandarion SEsfandyari-Manesh MAmin Marashi SMSamadi NFatemi SMAtyabi FEshraghi SDinarvRDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 221-234 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Jafarzadeh Kashi TS Eskandarion S Esfandyari-Manesh M Amin Marashi SM Samadi N Fatemi SM Atyabi F Eshraghi S Dinarv R Improved drug loading and antibacterial activity of minocycline-loaded PLGA nanoparticles prepared by solid/oil/water ion pairing method
description	Tahereh Sadat Jafarzadeh Kashi1, Solmaz Eskandarion1,2, Mehdi Esfandyari-Manesh3,4, Seyyed Mahmoud Amin Marashi5, Nasrin Samadi6, Seyyed Mostafa Fatemi1,7, Fatemeh Atyabi2,3, Saeed Eshraghi8, Rassoul Dinarvand2,31Dental Materials Department, Faculty of Dentistry, 2Department of Pharmaceutics, 3Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 4Department of Chemistry, Amirkabir University of Technology, Tehran, 5Department of Microbiology and Immunology, Faculty of Medicine, Babol University of Medical Sciences, Babol, 6Drug and Food Control Department, Faculty of Pharmacy, 7Research Center of Science and Technology in Medicine, 8Department of Microbiology, Tehran University of Medical Sciences, Tehran, IranBackground: Low drug entrapment efficiency of hydrophilic drugs into poly(lactic-co-glycolic acid) (PLGA) nanoparticles is a major drawback. The objective of this work was to investigate different methods of producing PLGA nanoparticles containing minocycline, a drug suitable for periodontal infections.Methods: Different methods, such as single and double solvent evaporation emulsion, ion pairing, and nanoprecipitation were used to prepare both PLGA and PEGylated PLGA nanoparticles. The resulting nanoparticles were analyzed for their morphology, particle size and size distribution, drug loading and entrapment efficiency, thermal properties, and antibacterial activity.Results: The nanoparticles prepared in this study were spherical, with an average particle size of 85&ndash;424 nm. The entrapment efficiency of the nanoparticles prepared using different methods was as follows: solid/oil/water ion pairing (29.9%) &gt; oil/oil (5.5%) &gt; water/oil/water (4.7%) &gt; modified oil/water (4.1%) &gt; nano precipitation (0.8%). Addition of dextran sulfate as an ion pairing agent, acting as an ionic spacer between PEGylated PLGA and minocycline, decreased the water solubility of minocycline, hence increasing the drug entrapment efficiency. Entrapment efficiency was also increased when low molecular weight PLGA and high molecular weight dextran sulfate was used. Drug release studies performed in phosphate buffer at pH 7.4 indicated slow release of minocycline from 3 days to several weeks. On antibacterial analysis, the minimum inhibitory concentration and minimum bactericidal concentration of nanoparticles was at least two times lower than that of the free drug.Conclusion: Novel minocycline-PEGylated PLGA nanoparticles prepared by the ion pairing method had the best drug loading and entrapment efficiency compared with other prepared nanoparticles. They also showed higher in vitro antibacterial activity than the free drug.Keywords: nanoparticle, PEGylation, PLGA, ion pairing, minocycline, antibacterial
format	article
author	Jafarzadeh Kashi TS Eskandarion S Esfandyari-Manesh M Amin Marashi SM Samadi N Fatemi SM Atyabi F Eshraghi S Dinarv R
author_facet	Jafarzadeh Kashi TS Eskandarion S Esfandyari-Manesh M Amin Marashi SM Samadi N Fatemi SM Atyabi F Eshraghi S Dinarv R
author_sort	Jafarzadeh Kashi TS
title	Improved drug loading and antibacterial activity of minocycline-loaded PLGA nanoparticles prepared by solid/oil/water ion pairing method
title_short	Improved drug loading and antibacterial activity of minocycline-loaded PLGA nanoparticles prepared by solid/oil/water ion pairing method
title_full	Improved drug loading and antibacterial activity of minocycline-loaded PLGA nanoparticles prepared by solid/oil/water ion pairing method
title_fullStr	Improved drug loading and antibacterial activity of minocycline-loaded PLGA nanoparticles prepared by solid/oil/water ion pairing method
title_full_unstemmed	Improved drug loading and antibacterial activity of minocycline-loaded PLGA nanoparticles prepared by solid/oil/water ion pairing method
title_sort	improved drug loading and antibacterial activity of minocycline-loaded plga nanoparticles prepared by solid/oil/water ion pairing method
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/fa264121f9654c71a6513191a2075bd2
work_keys_str_mv	AT jafarzadehkashits improveddrugloadingandantibacterialactivityofminocyclineloadedplgananoparticlespreparedbysolidoilwaterionpairingmethod AT eskandarions improveddrugloadingandantibacterialactivityofminocyclineloadedplgananoparticlespreparedbysolidoilwaterionpairingmethod AT esfandyarimaneshm improveddrugloadingandantibacterialactivityofminocyclineloadedplgananoparticlespreparedbysolidoilwaterionpairingmethod AT aminmarashism improveddrugloadingandantibacterialactivityofminocyclineloadedplgananoparticlespreparedbysolidoilwaterionpairingmethod AT samadin improveddrugloadingandantibacterialactivityofminocyclineloadedplgananoparticlespreparedbysolidoilwaterionpairingmethod AT fatemism improveddrugloadingandantibacterialactivityofminocyclineloadedplgananoparticlespreparedbysolidoilwaterionpairingmethod AT atyabif improveddrugloadingandantibacterialactivityofminocyclineloadedplgananoparticlespreparedbysolidoilwaterionpairingmethod AT eshraghis improveddrugloadingandantibacterialactivityofminocyclineloadedplgananoparticlespreparedbysolidoilwaterionpairingmethod AT dinarv improveddrugloadingandantibacterialactivityofminocyclineloadedplgananoparticlespreparedbysolidoilwaterionpairingmethod AT r improveddrugloadingandantibacterialactivityofminocyclineloadedplgananoparticlespreparedbysolidoilwaterionpairingmethod
_version_	1718402384749658112

Improved drug loading and antibacterial activity of minocycline-loaded PLGA nanoparticles prepared by solid/oil/water ion pairing method

Ejemplares similares