Expression of Specific Alleles of Zinc-Finger Transcription Factors, <i>HvSAP8</i> and <i>HvSAP16</i>, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations

Expression of Specific Alleles of Zinc-Finger Transcription Factors, <i>HvSAP8</i> and <i>HvSAP16</i>, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations

Two genes, <i>HvSAP8</i> and <i>HvSAP16</i>, encoding Zinc-finger proteins, were identified earlier as active in barley plants. Based on bioinformatics and sequencing analysis, six SNPs were found in the promoter regions of <i>HvSAP8</i> and one in <i>HvSAP1...

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Main Authors: Akmaral Baidyussen, Satyvaldy Jatayev, Gulmira Khassanova, Bekzak Amantayev, Grigory Sereda, Sergey Sereda, Narendra K. Gupta, Sunita Gupta, Carly Schramm, Peter Anderson, Colin L. D. Jenkins, Kathleen L. Soole, Peter Langridge, Yuri Shavrukov
Format: article
Language:EN
Published: MDPI AG 2021
Subjects:
ASQ and Amplifluor markers
barley
drought
gene expression
grain yield and yield components
SNP plant genotyping
Biology (General)
QH301-705.5
Chemistry
QD1-999
Online Access:https://doaj.org/article/fa27f56bbb5f4aa7a9a3378f3f21f42b
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Summary:Two genes, <i>HvSAP8</i> and <i>HvSAP16</i>, encoding Zinc-finger proteins, were identified earlier as active in barley plants. Based on bioinformatics and sequencing analysis, six SNPs were found in the promoter regions of <i>HvSAP8</i> and one in <i>HvSAP16</i>, among parents of two barley segregating populations, Granal × Baisheshek and Natali × Auksiniai-2. ASQ and Amplifluor markers were developed for <i>HvSAP8</i> and <i>HvSAP16</i>, one SNP in each gene, and in each of two populations, showing simple Mendelian segregation. Plants of F<sub>6</sub> selected breeding lines and parents were evaluated in a soil-based drought screen, revealing differential expression of <i>HvSAP8</i> and <i>HvSAP16</i> corresponding with the stress. After almost doubling expression during the early stages of stress, <i>HvSAP8</i> returned to pre-stress level or was strongly down-regulated in plants with Granal or Baisheshek genotypes, respectively. For <i>HvSAP16</i> under drought conditions, a high expression level was followed by either a return to original levels or strong down-regulation in plants with Natali or Auksiniai-2 genotypes, respectively. Grain yield in the same breeding lines and parents grown under moderate drought was strongly associated with their <i>HvSAP8</i> and <i>HvSAP16</i> genotypes. Additionally, Granal and Natali genotypes with specific alleles at <i>HvSAP8</i> and <i>HvSAP16</i> were associated with improved performance under drought via higher 1000 grain weight and more shoots per plant, respectively.

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