Expression of Specific Alleles of Zinc-Finger Transcription Factors, <i>HvSAP8</i> and <i>HvSAP16</i>, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations
Two genes, <i>HvSAP8</i> and <i>HvSAP16</i>, encoding Zinc-finger proteins, were identified earlier as active in barley plants. Based on bioinformatics and sequencing analysis, six SNPs were found in the promoter regions of <i>HvSAP8</i> and one in <i>HvSAP1...
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| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/fa27f56bbb5f4aa7a9a3378f3f21f42b |
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| Sumario: | Two genes, <i>HvSAP8</i> and <i>HvSAP16</i>, encoding Zinc-finger proteins, were identified earlier as active in barley plants. Based on bioinformatics and sequencing analysis, six SNPs were found in the promoter regions of <i>HvSAP8</i> and one in <i>HvSAP16</i>, among parents of two barley segregating populations, Granal × Baisheshek and Natali × Auksiniai-2. ASQ and Amplifluor markers were developed for <i>HvSAP8</i> and <i>HvSAP16</i>, one SNP in each gene, and in each of two populations, showing simple Mendelian segregation. Plants of F<sub>6</sub> selected breeding lines and parents were evaluated in a soil-based drought screen, revealing differential expression of <i>HvSAP8</i> and <i>HvSAP16</i> corresponding with the stress. After almost doubling expression during the early stages of stress, <i>HvSAP8</i> returned to pre-stress level or was strongly down-regulated in plants with Granal or Baisheshek genotypes, respectively. For <i>HvSAP16</i> under drought conditions, a high expression level was followed by either a return to original levels or strong down-regulation in plants with Natali or Auksiniai-2 genotypes, respectively. Grain yield in the same breeding lines and parents grown under moderate drought was strongly associated with their <i>HvSAP8</i> and <i>HvSAP16</i> genotypes. Additionally, Granal and Natali genotypes with specific alleles at <i>HvSAP8</i> and <i>HvSAP16</i> were associated with improved performance under drought via higher 1000 grain weight and more shoots per plant, respectively. |
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