Expression of Specific Alleles of Zinc-Finger Transcription Factors, HvSAP8 and HvSAP16, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations

Two genes, HvSAP8 and HvSAP16, encoding Zinc-finger proteins, were identified earlier as active in barley plants. Based on bioinformatics and sequencing analysis, six SNPs were found in the promoter regions of HvSAP8 and one in HvSAP1...

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Autores principales:	Akmaral Baidyussen, Satyvaldy Jatayev, Gulmira Khassanova, Bekzak Amantayev, Grigory Sereda, Sergey Sereda, Narendra K. Gupta, Sunita Gupta, Carly Schramm, Peter Anderson, Colin L. D. Jenkins, Kathleen L. Soole, Peter Langridge, Yuri Shavrukov
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ASQ and Amplifluor markers barley drought gene expression grain yield and yield components SNP plant genotyping Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/fa27f56bbb5f4aa7a9a3378f3f21f42b
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spelling	oai:doaj.org-article:fa27f56bbb5f4aa7a9a3378f3f21f42b2021-11-25T17:53:52ZExpression of Specific Alleles of Zinc-Finger Transcription Factors, <i>HvSAP8</i> and <i>HvSAP16</i>, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations10.3390/ijms2222121561422-00671661-6596https://doaj.org/article/fa27f56bbb5f4aa7a9a3378f3f21f42b2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12156https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Two genes, <i>HvSAP8</i> and <i>HvSAP16</i>, encoding Zinc-finger proteins, were identified earlier as active in barley plants. Based on bioinformatics and sequencing analysis, six SNPs were found in the promoter regions of <i>HvSAP8</i> and one in <i>HvSAP16</i>, among parents of two barley segregating populations, Granal × Baisheshek and Natali × Auksiniai-2. ASQ and Amplifluor markers were developed for <i>HvSAP8</i> and <i>HvSAP16</i>, one SNP in each gene, and in each of two populations, showing simple Mendelian segregation. Plants of F<sub>6</sub> selected breeding lines and parents were evaluated in a soil-based drought screen, revealing differential expression of <i>HvSAP8</i> and <i>HvSAP16</i> corresponding with the stress. After almost doubling expression during the early stages of stress, <i>HvSAP8</i> returned to pre-stress level or was strongly down-regulated in plants with Granal or Baisheshek genotypes, respectively. For <i>HvSAP16</i> under drought conditions, a high expression level was followed by either a return to original levels or strong down-regulation in plants with Natali or Auksiniai-2 genotypes, respectively. Grain yield in the same breeding lines and parents grown under moderate drought was strongly associated with their <i>HvSAP8</i> and <i>HvSAP16</i> genotypes. Additionally, Granal and Natali genotypes with specific alleles at <i>HvSAP8</i> and <i>HvSAP16</i> were associated with improved performance under drought via higher 1000 grain weight and more shoots per plant, respectively.Akmaral BaidyussenSatyvaldy JatayevGulmira KhassanovaBekzak AmantayevGrigory SeredaSergey SeredaNarendra K. GuptaSunita GuptaCarly SchrammPeter AndersonColin L. D. JenkinsKathleen L. SoolePeter LangridgeYuri ShavrukovMDPI AGarticleASQ and Amplifluor markersbarleydroughtgene expressiongrain yield and yield componentsSNP plant genotypingBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12156, p 12156 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ASQ and Amplifluor markers barley drought gene expression grain yield and yield components SNP plant genotyping Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	ASQ and Amplifluor markers barley drought gene expression grain yield and yield components SNP plant genotyping Biology (General) QH301-705.5 Chemistry QD1-999 Akmaral Baidyussen Satyvaldy Jatayev Gulmira Khassanova Bekzak Amantayev Grigory Sereda Sergey Sereda Narendra K. Gupta Sunita Gupta Carly Schramm Peter Anderson Colin L. D. Jenkins Kathleen L. Soole Peter Langridge Yuri Shavrukov Expression of Specific Alleles of Zinc-Finger Transcription Factors, <i>HvSAP8</i> and <i>HvSAP16</i>, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations
description	Two genes, <i>HvSAP8</i> and <i>HvSAP16</i>, encoding Zinc-finger proteins, were identified earlier as active in barley plants. Based on bioinformatics and sequencing analysis, six SNPs were found in the promoter regions of <i>HvSAP8</i> and one in <i>HvSAP16</i>, among parents of two barley segregating populations, Granal × Baisheshek and Natali × Auksiniai-2. ASQ and Amplifluor markers were developed for <i>HvSAP8</i> and <i>HvSAP16</i>, one SNP in each gene, and in each of two populations, showing simple Mendelian segregation. Plants of F<sub>6</sub> selected breeding lines and parents were evaluated in a soil-based drought screen, revealing differential expression of <i>HvSAP8</i> and <i>HvSAP16</i> corresponding with the stress. After almost doubling expression during the early stages of stress, <i>HvSAP8</i> returned to pre-stress level or was strongly down-regulated in plants with Granal or Baisheshek genotypes, respectively. For <i>HvSAP16</i> under drought conditions, a high expression level was followed by either a return to original levels or strong down-regulation in plants with Natali or Auksiniai-2 genotypes, respectively. Grain yield in the same breeding lines and parents grown under moderate drought was strongly associated with their <i>HvSAP8</i> and <i>HvSAP16</i> genotypes. Additionally, Granal and Natali genotypes with specific alleles at <i>HvSAP8</i> and <i>HvSAP16</i> were associated with improved performance under drought via higher 1000 grain weight and more shoots per plant, respectively.
format	article
author	Akmaral Baidyussen Satyvaldy Jatayev Gulmira Khassanova Bekzak Amantayev Grigory Sereda Sergey Sereda Narendra K. Gupta Sunita Gupta Carly Schramm Peter Anderson Colin L. D. Jenkins Kathleen L. Soole Peter Langridge Yuri Shavrukov
author_facet	Akmaral Baidyussen Satyvaldy Jatayev Gulmira Khassanova Bekzak Amantayev Grigory Sereda Sergey Sereda Narendra K. Gupta Sunita Gupta Carly Schramm Peter Anderson Colin L. D. Jenkins Kathleen L. Soole Peter Langridge Yuri Shavrukov
author_sort	Akmaral Baidyussen
title	Expression of Specific Alleles of Zinc-Finger Transcription Factors, <i>HvSAP8</i> and <i>HvSAP16</i>, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations
title_short	Expression of Specific Alleles of Zinc-Finger Transcription Factors, <i>HvSAP8</i> and <i>HvSAP16</i>, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations
title_full	Expression of Specific Alleles of Zinc-Finger Transcription Factors, <i>HvSAP8</i> and <i>HvSAP16</i>, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations
title_fullStr	Expression of Specific Alleles of Zinc-Finger Transcription Factors, <i>HvSAP8</i> and <i>HvSAP16</i>, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations
title_full_unstemmed	Expression of Specific Alleles of Zinc-Finger Transcription Factors, <i>HvSAP8</i> and <i>HvSAP16</i>, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations
title_sort	expression of specific alleles of zinc-finger transcription factors, <i>hvsap8</i> and <i>hvsap16</i>, and corresponding snp markers, are associated with drought tolerance in barley populations
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fa27f56bbb5f4aa7a9a3378f3f21f42b
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Expression of Specific Alleles of Zinc-Finger Transcription Factors, <i>HvSAP8</i> and <i>HvSAP16</i>, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations

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