Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody

Abstract Active collagen oligopeptides (ACOP) are bioactive collagen-derived peptides detected by a recently-established ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody use...

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Autores principales:	Yoshihiro Osawa, Kaho Nomura, Yoshifumi Kimira, Seiji Kushibe, Ken-ichi Takeyama, Masashi Nagao, Aya Kataoka-Matsushita, Seiko Koizumi, Hiroshi Mano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa2c70db6bd1427391fe9545c1b99e66
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spelling	oai:doaj.org-article:fa2c70db6bd1427391fe9545c1b99e662021-12-02T16:31:51ZMonitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody10.1038/s41598-021-92934-12045-2322https://doaj.org/article/fa2c70db6bd1427391fe9545c1b99e662021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92934-1https://doaj.org/toc/2045-2322Abstract Active collagen oligopeptides (ACOP) are bioactive collagen-derived peptides detected by a recently-established ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody used in this ELISA. We then investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC–MS/MS, and ELISA. We observed that the antibody showed strong cross-reactivity to Pro-Hyp and Gly-Pro-Hyp and weak cross-reactivity to commercial CP. CP ingestion increased the urinary level of ACOP over time, which correlated highly with urinary levels of peptide forms of Hyp and Pro-Hyp. Physical activity significantly decreased the urinary ACOP level. This study demonstrates changes in urinary ACOP following oral CP intake and physical activity using ELISA with the novel anti-ACOP antibody. Thus, ACOP may be useful as a new biomarker for collagen metabolism.Yoshihiro OsawaKaho NomuraYoshifumi KimiraSeiji KushibeKen-ichi TakeyamaMasashi NagaoAya Kataoka-MatsushitaSeiko KoizumiHiroshi ManoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoshihiro Osawa Kaho Nomura Yoshifumi Kimira Seiji Kushibe Ken-ichi Takeyama Masashi Nagao Aya Kataoka-Matsushita Seiko Koizumi Hiroshi Mano Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody
description	Abstract Active collagen oligopeptides (ACOP) are bioactive collagen-derived peptides detected by a recently-established ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody used in this ELISA. We then investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC–MS/MS, and ELISA. We observed that the antibody showed strong cross-reactivity to Pro-Hyp and Gly-Pro-Hyp and weak cross-reactivity to commercial CP. CP ingestion increased the urinary level of ACOP over time, which correlated highly with urinary levels of peptide forms of Hyp and Pro-Hyp. Physical activity significantly decreased the urinary ACOP level. This study demonstrates changes in urinary ACOP following oral CP intake and physical activity using ELISA with the novel anti-ACOP antibody. Thus, ACOP may be useful as a new biomarker for collagen metabolism.
format	article
author	Yoshihiro Osawa Kaho Nomura Yoshifumi Kimira Seiji Kushibe Ken-ichi Takeyama Masashi Nagao Aya Kataoka-Matsushita Seiko Koizumi Hiroshi Mano
author_facet	Yoshihiro Osawa Kaho Nomura Yoshifumi Kimira Seiji Kushibe Ken-ichi Takeyama Masashi Nagao Aya Kataoka-Matsushita Seiko Koizumi Hiroshi Mano
author_sort	Yoshihiro Osawa
title	Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody
title_short	Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody
title_full	Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody
title_fullStr	Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody
title_full_unstemmed	Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody
title_sort	monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using elisa with anti active collagen oligopeptide antibody
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fa2c70db6bd1427391fe9545c1b99e66
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Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody

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