A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary.

Systemic injection of a thymidine analogue such as bromodeoxyuridine (BrdU) in vertebrates is commonly used to detect and study cell production during development, adulthood, and pathology, particularly in studies of adult neurogenesis. Although researchers are applying this technique to multiple sp...

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Autores principales:	Jennifer M Barker, Thierry D Charlier, Gregory F Ball, Jacques Balthazart
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa2dc929d91549a18456da109c6806ba
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spelling	oai:doaj.org-article:fa2dc929d91549a18456da109c6806ba2021-11-18T07:45:49ZA new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary.1932-620310.1371/journal.pone.0063692https://doaj.org/article/fa2dc929d91549a18456da109c6806ba2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23691086/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Systemic injection of a thymidine analogue such as bromodeoxyuridine (BrdU) in vertebrates is commonly used to detect and study cell production during development, adulthood, and pathology, particularly in studies of adult neurogenesis. Although researchers are applying this technique to multiple species in various physiological conditions, the rate of BrdU clearance from the serum remains unknown in most cases. Changes in this clearance rate as a function of the species, sex or endocrine condition could however profoundly affect the interpretation of the results. We describe a rapid, sensitive, but simple bioassay for post-injection detection and quantification of BrdU in serum. This procedure was shown to be suitable for determining the length of time a thymidine analogue remains in the bloodstream of one avian species and seems applicable to any vertebrate provided sufficiently large blood samples can be collected. This technique was used to demonstrate that, in canaries, BrdU injected at a dose of 100 mg/kg is no longer available for incorporation into DNA between 30 and 60 min post-injection, a delay shorter than anticipated based on the available literature. Preliminary data suggest a similar fast clearance in Japanese quail and mice.Jennifer M BarkerThierry D CharlierGregory F BallJacques BalthazartPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63692 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jennifer M Barker Thierry D Charlier Gregory F Ball Jacques Balthazart A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary.
description	Systemic injection of a thymidine analogue such as bromodeoxyuridine (BrdU) in vertebrates is commonly used to detect and study cell production during development, adulthood, and pathology, particularly in studies of adult neurogenesis. Although researchers are applying this technique to multiple species in various physiological conditions, the rate of BrdU clearance from the serum remains unknown in most cases. Changes in this clearance rate as a function of the species, sex or endocrine condition could however profoundly affect the interpretation of the results. We describe a rapid, sensitive, but simple bioassay for post-injection detection and quantification of BrdU in serum. This procedure was shown to be suitable for determining the length of time a thymidine analogue remains in the bloodstream of one avian species and seems applicable to any vertebrate provided sufficiently large blood samples can be collected. This technique was used to demonstrate that, in canaries, BrdU injected at a dose of 100 mg/kg is no longer available for incorporation into DNA between 30 and 60 min post-injection, a delay shorter than anticipated based on the available literature. Preliminary data suggest a similar fast clearance in Japanese quail and mice.
format	article
author	Jennifer M Barker Thierry D Charlier Gregory F Ball Jacques Balthazart
author_facet	Jennifer M Barker Thierry D Charlier Gregory F Ball Jacques Balthazart
author_sort	Jennifer M Barker
title	A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary.
title_short	A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary.
title_full	A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary.
title_fullStr	A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary.
title_full_unstemmed	A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary.
title_sort	new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/fa2dc929d91549a18456da109c6806ba
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A new method for in vitro detection of bromodeoxyuridine in serum: a proof of concept in a songbird species, the canary.

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