Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization
Abstract Phage peptide display is a powerful technique for discovery of various target-specific ligands. However, target-unrelated peptides can often be obtained and cause ambiguous results. Peptide PB-TUP has been isolated repeatedly in our laboratory on different targets and we conducted a researc...
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2017
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oai:doaj.org-article:fa32cf24fe694719b2bcb838e6f45e392021-12-02T15:06:22ZDiscovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization10.1038/s41598-017-02891-x2045-2322https://doaj.org/article/fa32cf24fe694719b2bcb838e6f45e392017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02891-xhttps://doaj.org/toc/2045-2322Abstract Phage peptide display is a powerful technique for discovery of various target-specific ligands. However, target-unrelated peptides can often be obtained and cause ambiguous results. Peptide PB-TUP has been isolated repeatedly in our laboratory on different targets and we conducted a research on PB-TUP phage to investigate their binding properties and rate of propagation. ELISA and phage recovery assay demonstrated that PB-TUP phage had a significant superior affinity to polystyrene solid surface compared with control phage clones. In this study, some incidental bindings are excluded like blocking agents and non-specific binding of secondary antibodies. Propagation rate assays of the selected phage clones showed that the growth rate of PB-TUP phage was not superior to the control phages. Furthermore, the binding of PB-TUB to polystyrene was concentration dependent and varied with solution pH. Molecular modeling revealed that stable structures of α-helix and β-turn may contribute to the binding of PB-TUP to polystyrene plate. The PB-TUP sequence was fused to the N-terminus of peptide P2 and the fusion peptide significantly increased the binding affinity to polystyrene. The fusion peptide also enhanced the cell adhesion ability of peptide P2 with human umbilical vein endothelial cell (HUVEC). The addition of the polystyrene binding peptide provided a convenient method for peptide immobilization.Xu QiangKeyong SunLijun XingYifeng XuHong WangZhengpin ZhouJuan ZhangFang ZhangBilgen CaliskanMin WangZheng QiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
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Medicine R Science Q |
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Medicine R Science Q Xu Qiang Keyong Sun Lijun Xing Yifeng Xu Hong Wang Zhengpin Zhou Juan Zhang Fang Zhang Bilgen Caliskan Min Wang Zheng Qiu Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization |
| description |
Abstract Phage peptide display is a powerful technique for discovery of various target-specific ligands. However, target-unrelated peptides can often be obtained and cause ambiguous results. Peptide PB-TUP has been isolated repeatedly in our laboratory on different targets and we conducted a research on PB-TUP phage to investigate their binding properties and rate of propagation. ELISA and phage recovery assay demonstrated that PB-TUP phage had a significant superior affinity to polystyrene solid surface compared with control phage clones. In this study, some incidental bindings are excluded like blocking agents and non-specific binding of secondary antibodies. Propagation rate assays of the selected phage clones showed that the growth rate of PB-TUP phage was not superior to the control phages. Furthermore, the binding of PB-TUB to polystyrene was concentration dependent and varied with solution pH. Molecular modeling revealed that stable structures of α-helix and β-turn may contribute to the binding of PB-TUP to polystyrene plate. The PB-TUP sequence was fused to the N-terminus of peptide P2 and the fusion peptide significantly increased the binding affinity to polystyrene. The fusion peptide also enhanced the cell adhesion ability of peptide P2 with human umbilical vein endothelial cell (HUVEC). The addition of the polystyrene binding peptide provided a convenient method for peptide immobilization. |
| format |
article |
| author |
Xu Qiang Keyong Sun Lijun Xing Yifeng Xu Hong Wang Zhengpin Zhou Juan Zhang Fang Zhang Bilgen Caliskan Min Wang Zheng Qiu |
| author_facet |
Xu Qiang Keyong Sun Lijun Xing Yifeng Xu Hong Wang Zhengpin Zhou Juan Zhang Fang Zhang Bilgen Caliskan Min Wang Zheng Qiu |
| author_sort |
Xu Qiang |
| title |
Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization |
| title_short |
Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization |
| title_full |
Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization |
| title_fullStr |
Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization |
| title_full_unstemmed |
Discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization |
| title_sort |
discovery of a polystyrene binding peptide isolated from phage display library and its application in peptide immobilization |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/fa32cf24fe694719b2bcb838e6f45e39 |
| work_keys_str_mv |
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| _version_ |
1718388490446569472 |