Characterization of the vaginal micro- and mycobiome in asymptomatic reproductive-age Estonian women.

The application of high-throughput sequencing methods has raised doubt in the concept of the uniform healthy vaginal microbiota consisting predominantly of lactobacilli by revealing the existence of more variable bacterial community composition. As this needs to be analyzed more extensively and ther...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tiina Drell, Triin Lillsaar, Lea Tummeleht, Jaak Simm, Anu Aaspõllu, Edda Väin, Ivo Saarma, Andres Salumets, Gilbert G G Donders, Madis Metsis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa33f39aa9784ede8b3e65d8a14cdc72
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fa33f39aa9784ede8b3e65d8a14cdc72
record_format	dspace
spelling	oai:doaj.org-article:fa33f39aa9784ede8b3e65d8a14cdc722021-11-18T08:00:23ZCharacterization of the vaginal micro- and mycobiome in asymptomatic reproductive-age Estonian women.1932-620310.1371/journal.pone.0054379https://doaj.org/article/fa33f39aa9784ede8b3e65d8a14cdc722013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23372716/?tool=EBIhttps://doaj.org/toc/1932-6203The application of high-throughput sequencing methods has raised doubt in the concept of the uniform healthy vaginal microbiota consisting predominantly of lactobacilli by revealing the existence of more variable bacterial community composition. As this needs to be analyzed more extensively and there is little straightforward data regarding the vaginal mycobiome of asymptomatic women we aimed to define bacterial and fungal communities in vaginal samples from 494 asymptomatic, reproductive-age Estonian women. The composition of the vaginal microbiota was determined by amplifying bacterial 16S rRNA and fungal internal transcribed spacer-1 (ITS-1) regions and subsequently sequencing them using 454 Life Sciences pyrosequencing. We delineated five major bacterial community groups with distinctive diversity and species composition. Lactobacilli were among the most abundant bacteria in all groups, but also members of genus Gardnerella had high relative abundance in some of the groups. Microbial diversity increased with higher vaginal pH values, and was also higher when a malodorous discharge was present, indicating that some of the women who consider themselves healthy may potentially have asymptomatic bacterial vaginosis (BV). Our study is the first of its kind to analyze the mycobiome that colonizes the healthy vaginal environment using barcoded pyrosequencing technology. We observed 196 fungal operational taxonomic units (OTUs), including 16 OTUs of Candida spp., which is more diverse than previously recognized. However, assessing true fungal diversity was complicated because of the problems regarding the possible air-borne contamination and bioinformatics used for identification of fungal taxons as significant proportion of fungal sequences were assigned to unspecified OTUs.Tiina DrellTriin LillsaarLea TummelehtJaak SimmAnu AaspõlluEdda VäinIvo SaarmaAndres SalumetsGilbert G G DondersMadis MetsisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54379 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tiina Drell Triin Lillsaar Lea Tummeleht Jaak Simm Anu Aaspõllu Edda Väin Ivo Saarma Andres Salumets Gilbert G G Donders Madis Metsis Characterization of the vaginal micro- and mycobiome in asymptomatic reproductive-age Estonian women.
description	The application of high-throughput sequencing methods has raised doubt in the concept of the uniform healthy vaginal microbiota consisting predominantly of lactobacilli by revealing the existence of more variable bacterial community composition. As this needs to be analyzed more extensively and there is little straightforward data regarding the vaginal mycobiome of asymptomatic women we aimed to define bacterial and fungal communities in vaginal samples from 494 asymptomatic, reproductive-age Estonian women. The composition of the vaginal microbiota was determined by amplifying bacterial 16S rRNA and fungal internal transcribed spacer-1 (ITS-1) regions and subsequently sequencing them using 454 Life Sciences pyrosequencing. We delineated five major bacterial community groups with distinctive diversity and species composition. Lactobacilli were among the most abundant bacteria in all groups, but also members of genus Gardnerella had high relative abundance in some of the groups. Microbial diversity increased with higher vaginal pH values, and was also higher when a malodorous discharge was present, indicating that some of the women who consider themselves healthy may potentially have asymptomatic bacterial vaginosis (BV). Our study is the first of its kind to analyze the mycobiome that colonizes the healthy vaginal environment using barcoded pyrosequencing technology. We observed 196 fungal operational taxonomic units (OTUs), including 16 OTUs of Candida spp., which is more diverse than previously recognized. However, assessing true fungal diversity was complicated because of the problems regarding the possible air-borne contamination and bioinformatics used for identification of fungal taxons as significant proportion of fungal sequences were assigned to unspecified OTUs.
format	article
author	Tiina Drell Triin Lillsaar Lea Tummeleht Jaak Simm Anu Aaspõllu Edda Väin Ivo Saarma Andres Salumets Gilbert G G Donders Madis Metsis
author_facet	Tiina Drell Triin Lillsaar Lea Tummeleht Jaak Simm Anu Aaspõllu Edda Väin Ivo Saarma Andres Salumets Gilbert G G Donders Madis Metsis
author_sort	Tiina Drell
title	Characterization of the vaginal micro- and mycobiome in asymptomatic reproductive-age Estonian women.
title_short	Characterization of the vaginal micro- and mycobiome in asymptomatic reproductive-age Estonian women.
title_full	Characterization of the vaginal micro- and mycobiome in asymptomatic reproductive-age Estonian women.
title_fullStr	Characterization of the vaginal micro- and mycobiome in asymptomatic reproductive-age Estonian women.
title_full_unstemmed	Characterization of the vaginal micro- and mycobiome in asymptomatic reproductive-age Estonian women.
title_sort	characterization of the vaginal micro- and mycobiome in asymptomatic reproductive-age estonian women.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/fa33f39aa9784ede8b3e65d8a14cdc72
work_keys_str_mv	AT tiinadrell characterizationofthevaginalmicroandmycobiomeinasymptomaticreproductiveageestonianwomen AT triinlillsaar characterizationofthevaginalmicroandmycobiomeinasymptomaticreproductiveageestonianwomen AT leatummeleht characterizationofthevaginalmicroandmycobiomeinasymptomaticreproductiveageestonianwomen AT jaaksimm characterizationofthevaginalmicroandmycobiomeinasymptomaticreproductiveageestonianwomen AT anuaaspollu characterizationofthevaginalmicroandmycobiomeinasymptomaticreproductiveageestonianwomen AT eddavain characterizationofthevaginalmicroandmycobiomeinasymptomaticreproductiveageestonianwomen AT ivosaarma characterizationofthevaginalmicroandmycobiomeinasymptomaticreproductiveageestonianwomen AT andressalumets characterizationofthevaginalmicroandmycobiomeinasymptomaticreproductiveageestonianwomen AT gilbertggdonders characterizationofthevaginalmicroandmycobiomeinasymptomaticreproductiveageestonianwomen AT madismetsis characterizationofthevaginalmicroandmycobiomeinasymptomaticreproductiveageestonianwomen
_version_	1718422705770856448

Characterization of the vaginal micro- and mycobiome in asymptomatic reproductive-age Estonian women.

Ejemplares similares