High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics

Lemos Duarte et al. describe a strategy to produce, validate, and utilize highly-specific recombinant antibodies. As a proof of principle, they demonstrate development and validation of a panel of antibodies against proteins relevant to signaling by opiates. Their antibody-based tool can be useful i...

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Autores principales:	Mariana Lemos Duarte, Nikita A. Trimbake, Achla Gupta, Christine Tumanut, Xiaomin Fan, Catherine Woods, Akila Ram, Ivone Gomes, Erin N. Bobeck, Deborah Schechtman, Lakshmi A. Devi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fa3462494d824628b60f24925ecc50e1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

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spelling	oai:doaj.org-article:fa3462494d824628b60f24925ecc50e12021-12-02T14:13:39ZHigh-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics10.1038/s42003-021-01744-82399-3642https://doaj.org/article/fa3462494d824628b60f24925ecc50e12021-02-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01744-8https://doaj.org/toc/2399-3642Lemos Duarte et al. describe a strategy to produce, validate, and utilize highly-specific recombinant antibodies. As a proof of principle, they demonstrate development and validation of a panel of antibodies against proteins relevant to signaling by opiates. Their antibody-based tool can be useful in understanding the spatio-temporal dynamics of opioid signaling.Mariana Lemos DuarteNikita A. TrimbakeAchla GuptaChristine TumanutXiaomin FanCatherine WoodsAkila RamIvone GomesErin N. BobeckDeborah SchechtmanLakshmi A. DeviNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Mariana Lemos Duarte Nikita A. Trimbake Achla Gupta Christine Tumanut Xiaomin Fan Catherine Woods Akila Ram Ivone Gomes Erin N. Bobeck Deborah Schechtman Lakshmi A. Devi High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
description	Lemos Duarte et al. describe a strategy to produce, validate, and utilize highly-specific recombinant antibodies. As a proof of principle, they demonstrate development and validation of a panel of antibodies against proteins relevant to signaling by opiates. Their antibody-based tool can be useful in understanding the spatio-temporal dynamics of opioid signaling.
format	article
author	Mariana Lemos Duarte Nikita A. Trimbake Achla Gupta Christine Tumanut Xiaomin Fan Catherine Woods Akila Ram Ivone Gomes Erin N. Bobeck Deborah Schechtman Lakshmi A. Devi
author_facet	Mariana Lemos Duarte Nikita A. Trimbake Achla Gupta Christine Tumanut Xiaomin Fan Catherine Woods Akila Ram Ivone Gomes Erin N. Bobeck Deborah Schechtman Lakshmi A. Devi
author_sort	Mariana Lemos Duarte
title	High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
title_short	High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
title_full	High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
title_fullStr	High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
title_full_unstemmed	High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
title_sort	high-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fa3462494d824628b60f24925ecc50e1
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High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics

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