Fecal transplantation alleviates acute liver injury in mice through regulating Treg/Th17 cytokines balance

Abstract Changes in intestinal microecology during acute liver failure (ALF) directly affect the occurrence and development of the disease. The study aimed to investigate the relationship between the intestinal microbiota and the key immune cells. Fecal microbiota transplantation (FMT) was used to d...

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Autores principales:	Yongmei Liu, Linda Fan, Zhuo Cheng, Lei Yu, Shuo Cong, Yaxin Hu, Lili Zhu, Baofang Zhang, Yiju Cheng, Peiling Zhao, Xueke Zhao, Mingliang Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa3d813129194d06b562d0d0d5921088
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spelling	oai:doaj.org-article:fa3d813129194d06b562d0d0d59210882021-12-02T14:01:38ZFecal transplantation alleviates acute liver injury in mice through regulating Treg/Th17 cytokines balance10.1038/s41598-021-81263-y2045-2322https://doaj.org/article/fa3d813129194d06b562d0d0d59210882021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81263-yhttps://doaj.org/toc/2045-2322Abstract Changes in intestinal microecology during acute liver failure (ALF) directly affect the occurrence and development of the disease. The study aimed to investigate the relationship between the intestinal microbiota and the key immune cells. Fecal microbiota transplantation (FMT) was used to determine whether ALF can balance Th17/Treg cytokines. The relationship between gut microbiota and clinical indicators was analyzed. BALB/c mice were treated with d-galactosamine (d-GalN) to induce a murine ALF model. FMT to d-GalN mice was conducted to test for liver function indicators. Results showed that the proportions of Lachnospiraceae, Prevotella, S24-7, Odoribacter and Rikenellaceae in d-GalN mice with intestinal microbiota disorder were restored after FMT. Further, CIA analysis showed that bacteria had a covariant relationship with clinical indicators. Microbiota could account for changes in 49.9% of the overall clinical indicators. Adonis analysis showed that Ruminococcus, and Enterococcus have a greater impact on clinical indicators. FMT down-regulated the expression of IL-17A, TNF-α, and TGF-β, while up-regulated IL-10 and IL-22. Transplantation of feces from Saccharomyces boulardii donor mice improved GalN-induced liver damage. These findings indicate that FMT attenuates d-GalN-induced liver damage in mice, and a clinical trial is required to validate the relevance of our findings in humans, and to test whether this therapeutic approach is effective for patients with ALF.Yongmei LiuLinda FanZhuo ChengLei YuShuo CongYaxin HuLili ZhuBaofang ZhangYiju ChengPeiling ZhaoXueke ZhaoMingliang ChengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yongmei Liu Linda Fan Zhuo Cheng Lei Yu Shuo Cong Yaxin Hu Lili Zhu Baofang Zhang Yiju Cheng Peiling Zhao Xueke Zhao Mingliang Cheng Fecal transplantation alleviates acute liver injury in mice through regulating Treg/Th17 cytokines balance
description	Abstract Changes in intestinal microecology during acute liver failure (ALF) directly affect the occurrence and development of the disease. The study aimed to investigate the relationship between the intestinal microbiota and the key immune cells. Fecal microbiota transplantation (FMT) was used to determine whether ALF can balance Th17/Treg cytokines. The relationship between gut microbiota and clinical indicators was analyzed. BALB/c mice were treated with d-galactosamine (d-GalN) to induce a murine ALF model. FMT to d-GalN mice was conducted to test for liver function indicators. Results showed that the proportions of Lachnospiraceae, Prevotella, S24-7, Odoribacter and Rikenellaceae in d-GalN mice with intestinal microbiota disorder were restored after FMT. Further, CIA analysis showed that bacteria had a covariant relationship with clinical indicators. Microbiota could account for changes in 49.9% of the overall clinical indicators. Adonis analysis showed that Ruminococcus, and Enterococcus have a greater impact on clinical indicators. FMT down-regulated the expression of IL-17A, TNF-α, and TGF-β, while up-regulated IL-10 and IL-22. Transplantation of feces from Saccharomyces boulardii donor mice improved GalN-induced liver damage. These findings indicate that FMT attenuates d-GalN-induced liver damage in mice, and a clinical trial is required to validate the relevance of our findings in humans, and to test whether this therapeutic approach is effective for patients with ALF.
format	article
author	Yongmei Liu Linda Fan Zhuo Cheng Lei Yu Shuo Cong Yaxin Hu Lili Zhu Baofang Zhang Yiju Cheng Peiling Zhao Xueke Zhao Mingliang Cheng
author_facet	Yongmei Liu Linda Fan Zhuo Cheng Lei Yu Shuo Cong Yaxin Hu Lili Zhu Baofang Zhang Yiju Cheng Peiling Zhao Xueke Zhao Mingliang Cheng
author_sort	Yongmei Liu
title	Fecal transplantation alleviates acute liver injury in mice through regulating Treg/Th17 cytokines balance
title_short	Fecal transplantation alleviates acute liver injury in mice through regulating Treg/Th17 cytokines balance
title_full	Fecal transplantation alleviates acute liver injury in mice through regulating Treg/Th17 cytokines balance
title_fullStr	Fecal transplantation alleviates acute liver injury in mice through regulating Treg/Th17 cytokines balance
title_full_unstemmed	Fecal transplantation alleviates acute liver injury in mice through regulating Treg/Th17 cytokines balance
title_sort	fecal transplantation alleviates acute liver injury in mice through regulating treg/th17 cytokines balance
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fa3d813129194d06b562d0d0d5921088
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Fecal transplantation alleviates acute liver injury in mice through regulating Treg/Th17 cytokines balance

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