Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs.

Enterococci have the dual characteristics of being opportunistic pathogens and promising probiotics. The isolation from patients of CDC PNS-E2, a newly described Enterococcus species Enterococcus sanguinicola, may pose potential hazards. Enterococcus thailandicus from fermented sausage is a senior s...

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Autores principales: Xiaoying Wu, Bei Wu, Yue Li, Xiue Jin, Xiliang Wang
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Publicado: Public Library of Science (PLoS) 2021
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spelling oai:doaj.org-article:fa3ec2ad31b049659729595e6ca745252021-12-02T20:09:44ZIdentification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs.1932-620310.1371/journal.pone.0254081https://doaj.org/article/fa3ec2ad31b049659729595e6ca745252021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254081https://doaj.org/toc/1932-6203Enterococci have the dual characteristics of being opportunistic pathogens and promising probiotics. The isolation from patients of CDC PNS-E2, a newly described Enterococcus species Enterococcus sanguinicola, may pose potential hazards. Enterococcus thailandicus from fermented sausage is a senior subjective synonym of E. sanguinicola. In this study, Enterococcus thailandicus TC1 was first isolated in healthy pigs in Tongcheng, China and identified by phenotypic analysis and 16S rRNA-based techniques. To evaluate the strain safety, an approach including virulence factors, antibiotic resistance, and animal experiments was adopted. The results show that cylA, gelE, esp, agg, ace, efaAfm, efaAfs, ptsD genes were undetected, and that the strain was sensitive or poorly resistant to some clinically relevant antibiotics. However, the isolated strain demonstrated β-hemolytic activity in rabbit blood agar plates. Analysis of animal experiments revealed that the isolated strain had no adverse effect on translocation and the internal organ indices, though significant differences in histology (villi height, crypts height) of ileum were observed. The data acquired suggest that E. thailandicus TC1 may be associated with a potential health risk.Xiaoying WuBei WuYue LiXiue JinXiliang WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254081 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Xiaoying Wu
Bei Wu
Yue Li
Xiue Jin
Xiliang Wang
Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs.
description Enterococci have the dual characteristics of being opportunistic pathogens and promising probiotics. The isolation from patients of CDC PNS-E2, a newly described Enterococcus species Enterococcus sanguinicola, may pose potential hazards. Enterococcus thailandicus from fermented sausage is a senior subjective synonym of E. sanguinicola. In this study, Enterococcus thailandicus TC1 was first isolated in healthy pigs in Tongcheng, China and identified by phenotypic analysis and 16S rRNA-based techniques. To evaluate the strain safety, an approach including virulence factors, antibiotic resistance, and animal experiments was adopted. The results show that cylA, gelE, esp, agg, ace, efaAfm, efaAfs, ptsD genes were undetected, and that the strain was sensitive or poorly resistant to some clinically relevant antibiotics. However, the isolated strain demonstrated β-hemolytic activity in rabbit blood agar plates. Analysis of animal experiments revealed that the isolated strain had no adverse effect on translocation and the internal organ indices, though significant differences in histology (villi height, crypts height) of ileum were observed. The data acquired suggest that E. thailandicus TC1 may be associated with a potential health risk.
format article
author Xiaoying Wu
Bei Wu
Yue Li
Xiue Jin
Xiliang Wang
author_facet Xiaoying Wu
Bei Wu
Yue Li
Xiue Jin
Xiliang Wang
author_sort Xiaoying Wu
title Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs.
title_short Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs.
title_full Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs.
title_fullStr Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs.
title_full_unstemmed Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs.
title_sort identification and safety assessment of enterococcus thailandicus tc1 isolated from healthy pigs.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/fa3ec2ad31b049659729595e6ca74525
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AT beiwu identificationandsafetyassessmentofenterococcusthailandicustc1isolatedfromhealthypigs
AT yueli identificationandsafetyassessmentofenterococcusthailandicustc1isolatedfromhealthypigs
AT xiuejin identificationandsafetyassessmentofenterococcusthailandicustc1isolatedfromhealthypigs
AT xiliangwang identificationandsafetyassessmentofenterococcusthailandicustc1isolatedfromhealthypigs
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