Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs.
Enterococci have the dual characteristics of being opportunistic pathogens and promising probiotics. The isolation from patients of CDC PNS-E2, a newly described Enterococcus species Enterococcus sanguinicola, may pose potential hazards. Enterococcus thailandicus from fermented sausage is a senior s...
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oai:doaj.org-article:fa3ec2ad31b049659729595e6ca745252021-12-02T20:09:44ZIdentification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs.1932-620310.1371/journal.pone.0254081https://doaj.org/article/fa3ec2ad31b049659729595e6ca745252021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254081https://doaj.org/toc/1932-6203Enterococci have the dual characteristics of being opportunistic pathogens and promising probiotics. The isolation from patients of CDC PNS-E2, a newly described Enterococcus species Enterococcus sanguinicola, may pose potential hazards. Enterococcus thailandicus from fermented sausage is a senior subjective synonym of E. sanguinicola. In this study, Enterococcus thailandicus TC1 was first isolated in healthy pigs in Tongcheng, China and identified by phenotypic analysis and 16S rRNA-based techniques. To evaluate the strain safety, an approach including virulence factors, antibiotic resistance, and animal experiments was adopted. The results show that cylA, gelE, esp, agg, ace, efaAfm, efaAfs, ptsD genes were undetected, and that the strain was sensitive or poorly resistant to some clinically relevant antibiotics. However, the isolated strain demonstrated β-hemolytic activity in rabbit blood agar plates. Analysis of animal experiments revealed that the isolated strain had no adverse effect on translocation and the internal organ indices, though significant differences in histology (villi height, crypts height) of ileum were observed. The data acquired suggest that E. thailandicus TC1 may be associated with a potential health risk.Xiaoying WuBei WuYue LiXiue JinXiliang WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254081 (2021) |
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Medicine R Science Q Xiaoying Wu Bei Wu Yue Li Xiue Jin Xiliang Wang Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs. |
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Enterococci have the dual characteristics of being opportunistic pathogens and promising probiotics. The isolation from patients of CDC PNS-E2, a newly described Enterococcus species Enterococcus sanguinicola, may pose potential hazards. Enterococcus thailandicus from fermented sausage is a senior subjective synonym of E. sanguinicola. In this study, Enterococcus thailandicus TC1 was first isolated in healthy pigs in Tongcheng, China and identified by phenotypic analysis and 16S rRNA-based techniques. To evaluate the strain safety, an approach including virulence factors, antibiotic resistance, and animal experiments was adopted. The results show that cylA, gelE, esp, agg, ace, efaAfm, efaAfs, ptsD genes were undetected, and that the strain was sensitive or poorly resistant to some clinically relevant antibiotics. However, the isolated strain demonstrated β-hemolytic activity in rabbit blood agar plates. Analysis of animal experiments revealed that the isolated strain had no adverse effect on translocation and the internal organ indices, though significant differences in histology (villi height, crypts height) of ileum were observed. The data acquired suggest that E. thailandicus TC1 may be associated with a potential health risk. |
format |
article |
author |
Xiaoying Wu Bei Wu Yue Li Xiue Jin Xiliang Wang |
author_facet |
Xiaoying Wu Bei Wu Yue Li Xiue Jin Xiliang Wang |
author_sort |
Xiaoying Wu |
title |
Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs. |
title_short |
Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs. |
title_full |
Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs. |
title_fullStr |
Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs. |
title_full_unstemmed |
Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs. |
title_sort |
identification and safety assessment of enterococcus thailandicus tc1 isolated from healthy pigs. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doaj.org/article/fa3ec2ad31b049659729595e6ca74525 |
work_keys_str_mv |
AT xiaoyingwu identificationandsafetyassessmentofenterococcusthailandicustc1isolatedfromhealthypigs AT beiwu identificationandsafetyassessmentofenterococcusthailandicustc1isolatedfromhealthypigs AT yueli identificationandsafetyassessmentofenterococcusthailandicustc1isolatedfromhealthypigs AT xiuejin identificationandsafetyassessmentofenterococcusthailandicustc1isolatedfromhealthypigs AT xiliangwang identificationandsafetyassessmentofenterococcusthailandicustc1isolatedfromhealthypigs |
_version_ |
1718375100062892032 |