Efficacy and Molecular Effects of a Reduced Graphene Oxide/Fe3O4 Nanocomposite in Photothermal Therapy Against Cancer

Claudia C Barrera,1 Helena Groot,1 Watson L Vargas,2 Diana M Narváez1 1Human Genetics Laboratory, Department of Biological Sciences, Universidad de Los Andes, Bogotá, Colombia; 2Department of Chemical Engineering, Universidad de Los Andes, Bogotá, ColombiaCorresponde...

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Autores principales:	Barrera CC, Groot H, Vargas WL, Narváez DM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	reduced graphene oxide iron oxide photothermal therapy cell viability anti-apoptotic genes molecular effect. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fa43c443eea44fafb8cd23d5e622cb99
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spelling	oai:doaj.org-article:fa43c443eea44fafb8cd23d5e622cb992021-12-02T11:11:00ZEfficacy and Molecular Effects of a Reduced Graphene Oxide/Fe3O4 Nanocomposite in Photothermal Therapy Against Cancer1178-2013https://doaj.org/article/fa43c443eea44fafb8cd23d5e622cb992020-08-01T00:00:00Zhttps://www.dovepress.com/efficacy-and-molecular-effects-of-a-reduced-graphene-oxidefe3o4-nanoco-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Claudia C Barrera,1 Helena Groot,1 Watson L Vargas,2 Diana M Narváez1 1Human Genetics Laboratory, Department of Biological Sciences, Universidad de Los Andes, Bogotá, Colombia; 2Department of Chemical Engineering, Universidad de Los Andes, Bogotá, ColombiaCorrespondence: Claudia C BarreraHuman Genetics Laboratory, Department of Biological Sciences, Universidad de Los Andes, Cra. 1 # 18a-10, Bogotá, ColombiaTel +57 1 310 2936500Email cc.barrera1103@uniandes.edu.coPurpose: Expanded research on the biomedical applications of graphene has shown promising results, although interactions between cells and graphene are still unclear. The current study aims to dissect the cellular and molecular effects of graphene nanocomposite in photothermal therapy against cancer, and to evaluate its efficacy.Methods: In this study, a reduced graphene oxide and iron oxide (rGO-Fe3O4) nanocomposite was obtained by chemical synthesis. The nanocomposite was fully characterized by Raman spectroscopy, TEM, VSM and thermal profiling. Cell-nanocomposite interaction was evaluated by confocal microscopy and viability assays on cancer cell line HeLa. The efficacy of the thermal therapy and changes in gene expression of Bcl-2 and Hsp70 was assessed.Results: The resulting rGO-Fe3O4 nanocomposite exhibited superparamagnetic properties and the capacity to increase the surrounding temperature by 18– 20°C with respect to the initial temperature. The studies of cell-nanocomposite interaction showed that rGO-Fe3O4 attaches to cell membrane but there is a range of concentration at which the nanomaterial preserves cell viability. Photothermal therapy reduced cell viability to 32.6% and 23.7% with 50 and 100 μg/mL of nanomaterial, respectively. The effect of treatment on the molecular mechanism of cell death demonstrated an overexpression of anti-apoptotic proteins Hsp70 and Bcl-2 as an initial response to the therapy and depending on the aggressiveness of the treatment.Conclusion: The results of this study contribute to understanding the interactions between cell and graphene and support its application in photothermal therapy against cancer due to its promising results.Keywords: reduced graphene oxide, iron oxide, photothermal therapy, cell viability, anti-apoptotic genes, molecular effectBarrera CCGroot HVargas WLNarváez DMDove Medical Pressarticlereduced graphene oxideiron oxidephotothermal therapycell viabilityanti-apoptotic genesmolecular effect.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 6421-6432 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	reduced graphene oxide iron oxide photothermal therapy cell viability anti-apoptotic genes molecular effect. Medicine (General) R5-920
spellingShingle	reduced graphene oxide iron oxide photothermal therapy cell viability anti-apoptotic genes molecular effect. Medicine (General) R5-920 Barrera CC Groot H Vargas WL Narváez DM Efficacy and Molecular Effects of a Reduced Graphene Oxide/Fe3O4 Nanocomposite in Photothermal Therapy Against Cancer
description	Claudia C Barrera,1 Helena Groot,1 Watson L Vargas,2 Diana M Narváez1 1Human Genetics Laboratory, Department of Biological Sciences, Universidad de Los Andes, Bogotá, Colombia; 2Department of Chemical Engineering, Universidad de Los Andes, Bogotá, ColombiaCorrespondence: Claudia C BarreraHuman Genetics Laboratory, Department of Biological Sciences, Universidad de Los Andes, Cra. 1 # 18a-10, Bogotá, ColombiaTel +57 1 310 2936500Email cc.barrera1103@uniandes.edu.coPurpose: Expanded research on the biomedical applications of graphene has shown promising results, although interactions between cells and graphene are still unclear. The current study aims to dissect the cellular and molecular effects of graphene nanocomposite in photothermal therapy against cancer, and to evaluate its efficacy.Methods: In this study, a reduced graphene oxide and iron oxide (rGO-Fe3O4) nanocomposite was obtained by chemical synthesis. The nanocomposite was fully characterized by Raman spectroscopy, TEM, VSM and thermal profiling. Cell-nanocomposite interaction was evaluated by confocal microscopy and viability assays on cancer cell line HeLa. The efficacy of the thermal therapy and changes in gene expression of Bcl-2 and Hsp70 was assessed.Results: The resulting rGO-Fe3O4 nanocomposite exhibited superparamagnetic properties and the capacity to increase the surrounding temperature by 18– 20°C with respect to the initial temperature. The studies of cell-nanocomposite interaction showed that rGO-Fe3O4 attaches to cell membrane but there is a range of concentration at which the nanomaterial preserves cell viability. Photothermal therapy reduced cell viability to 32.6% and 23.7% with 50 and 100 μg/mL of nanomaterial, respectively. The effect of treatment on the molecular mechanism of cell death demonstrated an overexpression of anti-apoptotic proteins Hsp70 and Bcl-2 as an initial response to the therapy and depending on the aggressiveness of the treatment.Conclusion: The results of this study contribute to understanding the interactions between cell and graphene and support its application in photothermal therapy against cancer due to its promising results.Keywords: reduced graphene oxide, iron oxide, photothermal therapy, cell viability, anti-apoptotic genes, molecular effect
format	article
author	Barrera CC Groot H Vargas WL Narváez DM
author_facet	Barrera CC Groot H Vargas WL Narváez DM
author_sort	Barrera CC
title	Efficacy and Molecular Effects of a Reduced Graphene Oxide/Fe3O4 Nanocomposite in Photothermal Therapy Against Cancer
title_short	Efficacy and Molecular Effects of a Reduced Graphene Oxide/Fe3O4 Nanocomposite in Photothermal Therapy Against Cancer
title_full	Efficacy and Molecular Effects of a Reduced Graphene Oxide/Fe3O4 Nanocomposite in Photothermal Therapy Against Cancer
title_fullStr	Efficacy and Molecular Effects of a Reduced Graphene Oxide/Fe3O4 Nanocomposite in Photothermal Therapy Against Cancer
title_full_unstemmed	Efficacy and Molecular Effects of a Reduced Graphene Oxide/Fe3O4 Nanocomposite in Photothermal Therapy Against Cancer
title_sort	efficacy and molecular effects of a reduced graphene oxide/fe3o4 nanocomposite in photothermal therapy against cancer
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/fa43c443eea44fafb8cd23d5e622cb99
work_keys_str_mv	AT barreracc efficacyandmoleculareffectsofareducedgrapheneoxidefe3o4nanocompositeinphotothermaltherapyagainstcancer AT grooth efficacyandmoleculareffectsofareducedgrapheneoxidefe3o4nanocompositeinphotothermaltherapyagainstcancer AT vargaswl efficacyandmoleculareffectsofareducedgrapheneoxidefe3o4nanocompositeinphotothermaltherapyagainstcancer AT narvaezdm efficacyandmoleculareffectsofareducedgrapheneoxidefe3o4nanocompositeinphotothermaltherapyagainstcancer
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Efficacy and Molecular Effects of a Reduced Graphene Oxide/Fe3O4 Nanocomposite in Photothermal Therapy Against Cancer

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