Molecular record for the first authentication of Isaria cicadae from Vietnam

The entomopathogenic fungus T011, parasitizing on nymph of Cicada, collected in the coffee garden in Dak Lak Province, Vietnam, was preliminarily morphologically identified as Isaria cicadae, belonged to order Hypocreales and family Clavicipitaceae. To ensure the authenticity of T011, phylogenetic a...

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Autores principales:	Lao Thuan Duc, Van Trinh Hanh, Vuong Loi, Tien Vu Luyen, Huyen Le Thuy Ai, Dinh Hiep Minh, Truong Nguyen Binh
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	nuclear small ribosomal subunit nuclear large ribosomal subunit isaria cicadae phylogeny Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fa460935611e4e61a7088b90ad011f89
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spelling	oai:doaj.org-article:fa460935611e4e61a7088b90ad011f892021-12-05T14:10:41ZMolecular record for the first authentication of Isaria cicadae from Vietnam2391-541210.1515/biol-2021-0074https://doaj.org/article/fa460935611e4e61a7088b90ad011f892021-07-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0074https://doaj.org/toc/2391-5412The entomopathogenic fungus T011, parasitizing on nymph of Cicada, collected in the coffee garden in Dak Lak Province, Vietnam, was preliminarily morphologically identified as Isaria cicadae, belonged to order Hypocreales and family Clavicipitaceae. To ensure the authenticity of T011, phylogenetic analysis of the concatenated set of multiple genes including ITS, nrLSU, nrSSU, Rpb1, and Tef1 was applied to support the identification. Genomic DNA was isolated from dried sample T011. The PCR assay sequencing was applied to amplify ITS, nrLSU, nrSSU, Rpb1, and Tef1 gene. For phylogenetic analysis, the concatenated data of both target gens were constructed with MEGAX with a 1,000 replicate bootstrap based on the neighbor-joining, maximum likelihood, maximum parsimony method. As the result, the concatenated data containing 62 sequences belonged to order Hypocreales, families Clavicipitaceae, and 2 outgroup sequences belonged to order Hypocreales, genus Verticillium. The phylogenetic analysis results indicated that T011 was accepted at subclade Cordyceps and significantly formed the monophyletic group with referent Cordyceps cicadae (Telemorph of Isaria cicadae) with high bootstrap value. The phylogenetically analyzed result was strongly supported by our morphological analysis described as the Isaria cicadae. In summary, phylogenetic analyses based on the concatenated dataset were successfully applied to strengthen the identification of T011 as Isaria cicadae.Lao Thuan DucVan Trinh HanhVuong LoiTien Vu LuyenHuyen Le Thuy AiDinh Hiep MinhTruong Nguyen BinhDe Gruyterarticlenuclear small ribosomal subunitnuclear large ribosomal subunitisaria cicadaephylogenyBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 711-718 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nuclear small ribosomal subunit nuclear large ribosomal subunit isaria cicadae phylogeny Biology (General) QH301-705.5
spellingShingle	nuclear small ribosomal subunit nuclear large ribosomal subunit isaria cicadae phylogeny Biology (General) QH301-705.5 Lao Thuan Duc Van Trinh Hanh Vuong Loi Tien Vu Luyen Huyen Le Thuy Ai Dinh Hiep Minh Truong Nguyen Binh Molecular record for the first authentication of Isaria cicadae from Vietnam
description	The entomopathogenic fungus T011, parasitizing on nymph of Cicada, collected in the coffee garden in Dak Lak Province, Vietnam, was preliminarily morphologically identified as Isaria cicadae, belonged to order Hypocreales and family Clavicipitaceae. To ensure the authenticity of T011, phylogenetic analysis of the concatenated set of multiple genes including ITS, nrLSU, nrSSU, Rpb1, and Tef1 was applied to support the identification. Genomic DNA was isolated from dried sample T011. The PCR assay sequencing was applied to amplify ITS, nrLSU, nrSSU, Rpb1, and Tef1 gene. For phylogenetic analysis, the concatenated data of both target gens were constructed with MEGAX with a 1,000 replicate bootstrap based on the neighbor-joining, maximum likelihood, maximum parsimony method. As the result, the concatenated data containing 62 sequences belonged to order Hypocreales, families Clavicipitaceae, and 2 outgroup sequences belonged to order Hypocreales, genus Verticillium. The phylogenetic analysis results indicated that T011 was accepted at subclade Cordyceps and significantly formed the monophyletic group with referent Cordyceps cicadae (Telemorph of Isaria cicadae) with high bootstrap value. The phylogenetically analyzed result was strongly supported by our morphological analysis described as the Isaria cicadae. In summary, phylogenetic analyses based on the concatenated dataset were successfully applied to strengthen the identification of T011 as Isaria cicadae.
format	article
author	Lao Thuan Duc Van Trinh Hanh Vuong Loi Tien Vu Luyen Huyen Le Thuy Ai Dinh Hiep Minh Truong Nguyen Binh
author_facet	Lao Thuan Duc Van Trinh Hanh Vuong Loi Tien Vu Luyen Huyen Le Thuy Ai Dinh Hiep Minh Truong Nguyen Binh
author_sort	Lao Thuan Duc
title	Molecular record for the first authentication of Isaria cicadae from Vietnam
title_short	Molecular record for the first authentication of Isaria cicadae from Vietnam
title_full	Molecular record for the first authentication of Isaria cicadae from Vietnam
title_fullStr	Molecular record for the first authentication of Isaria cicadae from Vietnam
title_full_unstemmed	Molecular record for the first authentication of Isaria cicadae from Vietnam
title_sort	molecular record for the first authentication of isaria cicadae from vietnam
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/fa460935611e4e61a7088b90ad011f89
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Molecular record for the first authentication of Isaria cicadae from Vietnam

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