Cloning and characterization of the first serine carboxypeptidase from a plant parasitic nematode, Radopholus similis

Abstract Radopholus similis is an important parasitic nematode of plants. Serine carboxypeptidases (SCPs) are peptidases that hydrolyse peptides and proteins and play critical roles in the development, invasion, and pathogenesis of certain parasitic nematodes and other animal pathogens. In this stud...

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Autores principales:	Xin Huang, Chun-Ling Xu, Wan-Zhu Chen, Chun Chen, Hui Xie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa4cc9b52a144034b3f24b694423ca22
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spelling	oai:doaj.org-article:fa4cc9b52a144034b3f24b694423ca222021-12-02T15:05:13ZCloning and characterization of the first serine carboxypeptidase from a plant parasitic nematode, Radopholus similis10.1038/s41598-017-05093-72045-2322https://doaj.org/article/fa4cc9b52a144034b3f24b694423ca222017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05093-7https://doaj.org/toc/2045-2322Abstract Radopholus similis is an important parasitic nematode of plants. Serine carboxypeptidases (SCPs) are peptidases that hydrolyse peptides and proteins and play critical roles in the development, invasion, and pathogenesis of certain parasitic nematodes and other animal pathogens. In this study, we obtained the full-length sequence of the SCP gene from R. similis (Rs-scp-1), which is 1665 bp long and includes a 1461-bp open reading frames encoding 486 amino acids with an 18-aa signal peptide. This gene is a double-copy gene in R. similis. Rs-scp-1 was expressed in the procorpus, esophageal glands and intestines of females and in the esophageal glands and intestines of juveniles. Rs-scp-1 expression levels were highest in females, followed by juveniles and males, and lowest in eggs. Rs-scp-1 expression levels were significantly suppressed after R. similis was soaked in Rs-scp-1 dsRNA for 12 h. Nematodes were then inoculated into Anthurium andraeanum after RNAi treatment. Compared with water treatment, R. similis treated with RNAi were reduced in number and pathogenicity. In summary, we obtained the first SCP gene from a plant parasitic nematode and confirmed its role in the parasitic process.Xin HuangChun-Ling XuWan-Zhu ChenChun ChenHui XieNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xin Huang Chun-Ling Xu Wan-Zhu Chen Chun Chen Hui Xie Cloning and characterization of the first serine carboxypeptidase from a plant parasitic nematode, Radopholus similis
description	Abstract Radopholus similis is an important parasitic nematode of plants. Serine carboxypeptidases (SCPs) are peptidases that hydrolyse peptides and proteins and play critical roles in the development, invasion, and pathogenesis of certain parasitic nematodes and other animal pathogens. In this study, we obtained the full-length sequence of the SCP gene from R. similis (Rs-scp-1), which is 1665 bp long and includes a 1461-bp open reading frames encoding 486 amino acids with an 18-aa signal peptide. This gene is a double-copy gene in R. similis. Rs-scp-1 was expressed in the procorpus, esophageal glands and intestines of females and in the esophageal glands and intestines of juveniles. Rs-scp-1 expression levels were highest in females, followed by juveniles and males, and lowest in eggs. Rs-scp-1 expression levels were significantly suppressed after R. similis was soaked in Rs-scp-1 dsRNA for 12 h. Nematodes were then inoculated into Anthurium andraeanum after RNAi treatment. Compared with water treatment, R. similis treated with RNAi were reduced in number and pathogenicity. In summary, we obtained the first SCP gene from a plant parasitic nematode and confirmed its role in the parasitic process.
format	article
author	Xin Huang Chun-Ling Xu Wan-Zhu Chen Chun Chen Hui Xie
author_facet	Xin Huang Chun-Ling Xu Wan-Zhu Chen Chun Chen Hui Xie
author_sort	Xin Huang
title	Cloning and characterization of the first serine carboxypeptidase from a plant parasitic nematode, Radopholus similis
title_short	Cloning and characterization of the first serine carboxypeptidase from a plant parasitic nematode, Radopholus similis
title_full	Cloning and characterization of the first serine carboxypeptidase from a plant parasitic nematode, Radopholus similis
title_fullStr	Cloning and characterization of the first serine carboxypeptidase from a plant parasitic nematode, Radopholus similis
title_full_unstemmed	Cloning and characterization of the first serine carboxypeptidase from a plant parasitic nematode, Radopholus similis
title_sort	cloning and characterization of the first serine carboxypeptidase from a plant parasitic nematode, radopholus similis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fa4cc9b52a144034b3f24b694423ca22
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Cloning and characterization of the first serine carboxypeptidase from a plant parasitic nematode, Radopholus similis

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