A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin

Boonnada Pamornpathomkul,1 Adisak Wongkajornsilp,2 Wanida Laiwattanapaisal,3 Theerasak Rojanarata,1 Praneet Opanasopit,1 Tanasait Ngawhirunpat1 1Department of Pharmaceutical Technology, Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group, Silpakorn University, Nakhon Pathom,...

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Autores principales:	Pamornpathomkul B, Wongkajornsilp A, Laiwattanapaisal W, Rojanarata T, Opanasopit P, Ngawhirunpat T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Hollow microneedle Solid microneedle Electroporation Plasmid Ovalbumin Skin immunization Nanocarrier Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fa611eea63a447e1b5398974630a22d2
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spelling	oai:doaj.org-article:fa611eea63a447e1b5398974630a22d22021-12-02T02:10:26ZA combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin1178-2013https://doaj.org/article/fa611eea63a447e1b5398974630a22d22017-01-01T00:00:00Zhttps://www.dovepress.com/a-combined-approach-of-hollow-microneedles-and-nanocarriers-for-skin-i-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Boonnada Pamornpathomkul,1 Adisak Wongkajornsilp,2 Wanida Laiwattanapaisal,3 Theerasak Rojanarata,1 Praneet Opanasopit,1 Tanasait Ngawhirunpat1 1Department of Pharmaceutical Technology, Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group, Silpakorn University, Nakhon Pathom, 2Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 3Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand Abstract: The aim of this study was to investigate the use of different types of microneedles (MNs) and nanocarriers for in vitro skin permeation and in vivo immunization of plasmid DNA encoding ovalbumin (pOVA). In vitro skin permeation studies indicated that hollow MNs had a superior enhancing effect on skin permeation compared with solid MN patches, electroporation (EP) patches, the combination of MN and EP patches, and untreated skin. Upon using hollow MNs combined with nanocarriers for pOVA delivery, the skin permeation was higher than for the delivery of naked pOVA, as evidenced by the increased amount of pOVA in Franz diffusion cells and immunoglobulin G (IgG) antibody responses. When the hollow MNs were used for the delivery of nanocarrier:pOVA complexes into the skin of mice, they induced a stronger IgG immune response than conventional subcutaneous (SC) injections. In addition, immunization of mice with the hollow MNs did not induce signs of skin infection or pinpoint bleeding. Accordingly, the hollow MNs combined with a nanocarrier delivery system is a promising approach for delivering pOVA complexes to the skin for promoting successful immunization. Keywords: hollow microneedle, solid microneedle, electroporation, plasmid DNA encoding ovalbumin, skin immunization, nanocarrierPamornpathomkul BWongkajornsilp ALaiwattanapaisal WRojanarata TOpanasopit PNgawhirunpat TDove Medical PressarticleHollow microneedleSolid microneedleElectroporationPlasmid OvalbuminSkin immunizationNanocarrierMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 885-898 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hollow microneedle Solid microneedle Electroporation Plasmid Ovalbumin Skin immunization Nanocarrier Medicine (General) R5-920
spellingShingle	Hollow microneedle Solid microneedle Electroporation Plasmid Ovalbumin Skin immunization Nanocarrier Medicine (General) R5-920 Pamornpathomkul B Wongkajornsilp A Laiwattanapaisal W Rojanarata T Opanasopit P Ngawhirunpat T A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin
description	Boonnada Pamornpathomkul,1 Adisak Wongkajornsilp,2 Wanida Laiwattanapaisal,3 Theerasak Rojanarata,1 Praneet Opanasopit,1 Tanasait Ngawhirunpat1 1Department of Pharmaceutical Technology, Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group, Silpakorn University, Nakhon Pathom, 2Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 3Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand Abstract: The aim of this study was to investigate the use of different types of microneedles (MNs) and nanocarriers for in vitro skin permeation and in vivo immunization of plasmid DNA encoding ovalbumin (pOVA). In vitro skin permeation studies indicated that hollow MNs had a superior enhancing effect on skin permeation compared with solid MN patches, electroporation (EP) patches, the combination of MN and EP patches, and untreated skin. Upon using hollow MNs combined with nanocarriers for pOVA delivery, the skin permeation was higher than for the delivery of naked pOVA, as evidenced by the increased amount of pOVA in Franz diffusion cells and immunoglobulin G (IgG) antibody responses. When the hollow MNs were used for the delivery of nanocarrier:pOVA complexes into the skin of mice, they induced a stronger IgG immune response than conventional subcutaneous (SC) injections. In addition, immunization of mice with the hollow MNs did not induce signs of skin infection or pinpoint bleeding. Accordingly, the hollow MNs combined with a nanocarrier delivery system is a promising approach for delivering pOVA complexes to the skin for promoting successful immunization. Keywords: hollow microneedle, solid microneedle, electroporation, plasmid DNA encoding ovalbumin, skin immunization, nanocarrier
format	article
author	Pamornpathomkul B Wongkajornsilp A Laiwattanapaisal W Rojanarata T Opanasopit P Ngawhirunpat T
author_facet	Pamornpathomkul B Wongkajornsilp A Laiwattanapaisal W Rojanarata T Opanasopit P Ngawhirunpat T
author_sort	Pamornpathomkul B
title	A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin
title_short	A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin
title_full	A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin
title_fullStr	A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin
title_full_unstemmed	A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin
title_sort	combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid dna encoding ovalbumin
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/fa611eea63a447e1b5398974630a22d2
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A combined approach of hollow microneedles and nanocarriers for skin immunization with plasmid DNA encoding ovalbumin

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