Stable fabrication of a large nanopore by controlled dielectric breakdown in a high-pH solution for the detection of various-sized molecules

Abstract For nanopore sensing of various-sized molecules with high sensitivity, the size of the nanopore should be adjusted according to the size of each target molecule. For solid-state nanopores, a simple and inexpensive nanopore fabrication method utilizing dielectric breakdown of a membrane is w...

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Autores principales:	Itaru Yanagi, Rena Akahori, Ken-ichi Takeda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fa620ac505f24f958fcde37bfecb279a
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spelling	oai:doaj.org-article:fa620ac505f24f958fcde37bfecb279a2021-12-02T16:08:05ZStable fabrication of a large nanopore by controlled dielectric breakdown in a high-pH solution for the detection of various-sized molecules10.1038/s41598-019-49622-y2045-2322https://doaj.org/article/fa620ac505f24f958fcde37bfecb279a2019-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-49622-yhttps://doaj.org/toc/2045-2322Abstract For nanopore sensing of various-sized molecules with high sensitivity, the size of the nanopore should be adjusted according to the size of each target molecule. For solid-state nanopores, a simple and inexpensive nanopore fabrication method utilizing dielectric breakdown of a membrane is widely used. This method is suitable for fabricating a small nanopore. However, it suffers two serious problems when attempting to fabricate a large nanopore: the generation of multiple nanopores and the non-opening failure of a nanopore. In this study, we found that nanopore fabrication by dielectric breakdown of a SiN membrane under high-pH conditions (pH ≥ 11.3) could overcome these two problems and enabled the formation of a single large nanopore up to 40 nm in diameter within one minute. Moreover, the ionic-current blockades derived from streptavidin-labelled and non-labelled DNA passing through the fabricated nanopore were clearly distinguished. The current blockades caused by streptavidin-labelled DNA could be identified even when its concentration is 1% of the total DNA.Itaru YanagiRena AkahoriKen-ichi TakedaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-15 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Itaru Yanagi Rena Akahori Ken-ichi Takeda Stable fabrication of a large nanopore by controlled dielectric breakdown in a high-pH solution for the detection of various-sized molecules
description	Abstract For nanopore sensing of various-sized molecules with high sensitivity, the size of the nanopore should be adjusted according to the size of each target molecule. For solid-state nanopores, a simple and inexpensive nanopore fabrication method utilizing dielectric breakdown of a membrane is widely used. This method is suitable for fabricating a small nanopore. However, it suffers two serious problems when attempting to fabricate a large nanopore: the generation of multiple nanopores and the non-opening failure of a nanopore. In this study, we found that nanopore fabrication by dielectric breakdown of a SiN membrane under high-pH conditions (pH ≥ 11.3) could overcome these two problems and enabled the formation of a single large nanopore up to 40 nm in diameter within one minute. Moreover, the ionic-current blockades derived from streptavidin-labelled and non-labelled DNA passing through the fabricated nanopore were clearly distinguished. The current blockades caused by streptavidin-labelled DNA could be identified even when its concentration is 1% of the total DNA.
format	article
author	Itaru Yanagi Rena Akahori Ken-ichi Takeda
author_facet	Itaru Yanagi Rena Akahori Ken-ichi Takeda
author_sort	Itaru Yanagi
title	Stable fabrication of a large nanopore by controlled dielectric breakdown in a high-pH solution for the detection of various-sized molecules
title_short	Stable fabrication of a large nanopore by controlled dielectric breakdown in a high-pH solution for the detection of various-sized molecules
title_full	Stable fabrication of a large nanopore by controlled dielectric breakdown in a high-pH solution for the detection of various-sized molecules
title_fullStr	Stable fabrication of a large nanopore by controlled dielectric breakdown in a high-pH solution for the detection of various-sized molecules
title_full_unstemmed	Stable fabrication of a large nanopore by controlled dielectric breakdown in a high-pH solution for the detection of various-sized molecules
title_sort	stable fabrication of a large nanopore by controlled dielectric breakdown in a high-ph solution for the detection of various-sized molecules
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/fa620ac505f24f958fcde37bfecb279a
work_keys_str_mv	AT itaruyanagi stablefabricationofalargenanoporebycontrolleddielectricbreakdowninahighphsolutionforthedetectionofvarioussizedmolecules AT renaakahori stablefabricationofalargenanoporebycontrolleddielectricbreakdowninahighphsolutionforthedetectionofvarioussizedmolecules AT kenichitakeda stablefabricationofalargenanoporebycontrolleddielectricbreakdowninahighphsolutionforthedetectionofvarioussizedmolecules
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Stable fabrication of a large nanopore by controlled dielectric breakdown in a high-pH solution for the detection of various-sized molecules

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