Multi-tissue omics analyses reveal molecular regulatory networks for puberty in composite beef cattle.
Puberty is a complex physiological event by which animals mature into an adult capable of sexual reproduction. In order to enhance our understanding of the genes and regulatory pathways and networks involved in puberty, we characterized the transcriptome of five reproductive tissues (i.e. hypothalam...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2014
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/fa69c5c599b54c368f6a947bb47f1b1e |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:fa69c5c599b54c368f6a947bb47f1b1e |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:fa69c5c599b54c368f6a947bb47f1b1e2021-11-25T06:07:43ZMulti-tissue omics analyses reveal molecular regulatory networks for puberty in composite beef cattle.1932-620310.1371/journal.pone.0102551https://doaj.org/article/fa69c5c599b54c368f6a947bb47f1b1e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25048735/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Puberty is a complex physiological event by which animals mature into an adult capable of sexual reproduction. In order to enhance our understanding of the genes and regulatory pathways and networks involved in puberty, we characterized the transcriptome of five reproductive tissues (i.e. hypothalamus, pituitary gland, ovary, uterus, and endometrium) as well as tissues known to be relevant to growth and metabolism needed to achieve puberty (i.e., longissimus dorsi muscle, adipose, and liver). These tissues were collected from pre- and post-pubertal Brangus heifers (3/8 Brahman; Bos indicus x 5/8 Angus; Bos taurus) derived from a population of cattle used to identify quantitative trait loci associated with fertility traits (i.e., age of first observed corpus luteum (ACL), first service conception (FSC), and heifer pregnancy (HPG)). In order to exploit the power of complementary omics analyses, pre- and post-puberty co-expression gene networks were constructed by combining the results from genome-wide association studies (GWAS), RNA-Seq, and bovine transcription factors. Eight tissues among pre-pubertal and post-pubertal Brangus heifers revealed 1,515 differentially expressed and 943 tissue-specific genes within the 17,832 genes confirmed by RNA-Seq analysis. The hypothalamus experienced the most notable up-regulation of genes via puberty (i.e., 204 out of 275 genes). Combining the results of GWAS and RNA-Seq, we identified 25 loci containing a single nucleotide polymorphism (SNP) associated with ACL, FSC, and (or) HPG. Seventeen of these SNP were within a gene and 13 of the genes were expressed in uterus or endometrium. Multi-tissue omics analyses revealed 2,450 co-expressed genes relative to puberty. The pre-pubertal network had 372,861 connections whereas the post-pubertal network had 328,357 connections. A sub-network from this process revealed key transcriptional regulators (i.e., PITX2, FOXA1, DACH2, PROP1, SIX6, etc.). Results from these multi-tissue omics analyses improve understanding of the number of genes and their complex interactions for puberty in cattle.Angela CánovasAntonio ReverterKasey L DeAtleyRyan L AshleyMichelle L ColgraveMarina R S FortesAlma Islas-TrejoSigrid LehnertLaercio Porto-NetoGonzalo RincónGail A SilverWarren M SnellingJuan F MedranoMilton G ThomasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e102551 (2014) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Angela Cánovas Antonio Reverter Kasey L DeAtley Ryan L Ashley Michelle L Colgrave Marina R S Fortes Alma Islas-Trejo Sigrid Lehnert Laercio Porto-Neto Gonzalo Rincón Gail A Silver Warren M Snelling Juan F Medrano Milton G Thomas Multi-tissue omics analyses reveal molecular regulatory networks for puberty in composite beef cattle. |
| description |
Puberty is a complex physiological event by which animals mature into an adult capable of sexual reproduction. In order to enhance our understanding of the genes and regulatory pathways and networks involved in puberty, we characterized the transcriptome of five reproductive tissues (i.e. hypothalamus, pituitary gland, ovary, uterus, and endometrium) as well as tissues known to be relevant to growth and metabolism needed to achieve puberty (i.e., longissimus dorsi muscle, adipose, and liver). These tissues were collected from pre- and post-pubertal Brangus heifers (3/8 Brahman; Bos indicus x 5/8 Angus; Bos taurus) derived from a population of cattle used to identify quantitative trait loci associated with fertility traits (i.e., age of first observed corpus luteum (ACL), first service conception (FSC), and heifer pregnancy (HPG)). In order to exploit the power of complementary omics analyses, pre- and post-puberty co-expression gene networks were constructed by combining the results from genome-wide association studies (GWAS), RNA-Seq, and bovine transcription factors. Eight tissues among pre-pubertal and post-pubertal Brangus heifers revealed 1,515 differentially expressed and 943 tissue-specific genes within the 17,832 genes confirmed by RNA-Seq analysis. The hypothalamus experienced the most notable up-regulation of genes via puberty (i.e., 204 out of 275 genes). Combining the results of GWAS and RNA-Seq, we identified 25 loci containing a single nucleotide polymorphism (SNP) associated with ACL, FSC, and (or) HPG. Seventeen of these SNP were within a gene and 13 of the genes were expressed in uterus or endometrium. Multi-tissue omics analyses revealed 2,450 co-expressed genes relative to puberty. The pre-pubertal network had 372,861 connections whereas the post-pubertal network had 328,357 connections. A sub-network from this process revealed key transcriptional regulators (i.e., PITX2, FOXA1, DACH2, PROP1, SIX6, etc.). Results from these multi-tissue omics analyses improve understanding of the number of genes and their complex interactions for puberty in cattle. |
| format |
article |
| author |
Angela Cánovas Antonio Reverter Kasey L DeAtley Ryan L Ashley Michelle L Colgrave Marina R S Fortes Alma Islas-Trejo Sigrid Lehnert Laercio Porto-Neto Gonzalo Rincón Gail A Silver Warren M Snelling Juan F Medrano Milton G Thomas |
| author_facet |
Angela Cánovas Antonio Reverter Kasey L DeAtley Ryan L Ashley Michelle L Colgrave Marina R S Fortes Alma Islas-Trejo Sigrid Lehnert Laercio Porto-Neto Gonzalo Rincón Gail A Silver Warren M Snelling Juan F Medrano Milton G Thomas |
| author_sort |
Angela Cánovas |
| title |
Multi-tissue omics analyses reveal molecular regulatory networks for puberty in composite beef cattle. |
| title_short |
Multi-tissue omics analyses reveal molecular regulatory networks for puberty in composite beef cattle. |
| title_full |
Multi-tissue omics analyses reveal molecular regulatory networks for puberty in composite beef cattle. |
| title_fullStr |
Multi-tissue omics analyses reveal molecular regulatory networks for puberty in composite beef cattle. |
| title_full_unstemmed |
Multi-tissue omics analyses reveal molecular regulatory networks for puberty in composite beef cattle. |
| title_sort |
multi-tissue omics analyses reveal molecular regulatory networks for puberty in composite beef cattle. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/fa69c5c599b54c368f6a947bb47f1b1e |
| work_keys_str_mv |
AT angelacanovas multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle AT antonioreverter multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle AT kaseyldeatley multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle AT ryanlashley multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle AT michellelcolgrave multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle AT marinarsfortes multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle AT almaislastrejo multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle AT sigridlehnert multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle AT laercioportoneto multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle AT gonzalorincon multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle AT gailasilver multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle AT warrenmsnelling multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle AT juanfmedrano multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle AT miltongthomas multitissueomicsanalysesrevealmolecularregulatorynetworksforpubertyincompositebeefcattle |
| _version_ |
1718414200360927232 |