Curium(III) speciation in the presence of microbial cell wall components

Trivalent actinides such as Cm(III) are able to strongly interact with microbes and especially with bacterial cell walls. However, detailed knowledge of the influence of different cell wall components is somewhat lacking. For this investigation, we studied the formation of aqueous Cm(III) complexes...

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Autores principales:	Henry Moll, Astrid Barkleit, Laura Frost, Johannes Raff
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Curium Lipopolysaccharide Peptidoglycan Plasma membrane Bacterial cell walls Luminescence spectroscopy Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/fa6b5d3f43db40ceac768ab3a0941a9e
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spelling	oai:doaj.org-article:fa6b5d3f43db40ceac768ab3a0941a9e2021-11-06T04:14:50ZCurium(III) speciation in the presence of microbial cell wall components0147-651310.1016/j.ecoenv.2021.112887https://doaj.org/article/fa6b5d3f43db40ceac768ab3a0941a9e2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321009994https://doaj.org/toc/0147-6513Trivalent actinides such as Cm(III) are able to strongly interact with microbes and especially with bacterial cell walls. However, detailed knowledge of the influence of different cell wall components is somewhat lacking. For this investigation, we studied the formation of aqueous Cm(III) complexes with cell wall components (e.g., lipopolysaccharide, peptidoglycan, and plasma membranes) using time-resolved laser-induced fluorescence spectroscopy (TRLFS). For all systems, two specific Cm(III) complexes with the biomacromolecules were observed as a function of pH. Specifically, Cm(III) was found to bind to phosphate and carboxyl groups present in the structure of the biomacromolecules. Stability constants and luminescence parameters of the specific Cm(III) complexes were determined and are presented. The pH of the surrounding aqueous solution, the plasma membrane concentration, and proteins included in the crude plasma membrane fraction were found to significantly impact the complexation of Cm(III). The Cm(III) luminescence spectra with plasma membranes, cell wall polymers, as well as Gram-negative (Sporomusa sp. MT-2.99 and Pseudomonas fluorescens) and Gram-positive (Paenibacillus sp. MT-2.2) bacteria will be explained by linear combination fitting using the investigated components.Henry MollAstrid BarkleitLaura FrostJohannes RaffElsevierarticleCuriumLipopolysaccharidePeptidoglycanPlasma membraneBacterial cell wallsLuminescence spectroscopyEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112887- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Curium Lipopolysaccharide Peptidoglycan Plasma membrane Bacterial cell walls Luminescence spectroscopy Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Curium Lipopolysaccharide Peptidoglycan Plasma membrane Bacterial cell walls Luminescence spectroscopy Environmental pollution TD172-193.5 Environmental sciences GE1-350 Henry Moll Astrid Barkleit Laura Frost Johannes Raff Curium(III) speciation in the presence of microbial cell wall components
description	Trivalent actinides such as Cm(III) are able to strongly interact with microbes and especially with bacterial cell walls. However, detailed knowledge of the influence of different cell wall components is somewhat lacking. For this investigation, we studied the formation of aqueous Cm(III) complexes with cell wall components (e.g., lipopolysaccharide, peptidoglycan, and plasma membranes) using time-resolved laser-induced fluorescence spectroscopy (TRLFS). For all systems, two specific Cm(III) complexes with the biomacromolecules were observed as a function of pH. Specifically, Cm(III) was found to bind to phosphate and carboxyl groups present in the structure of the biomacromolecules. Stability constants and luminescence parameters of the specific Cm(III) complexes were determined and are presented. The pH of the surrounding aqueous solution, the plasma membrane concentration, and proteins included in the crude plasma membrane fraction were found to significantly impact the complexation of Cm(III). The Cm(III) luminescence spectra with plasma membranes, cell wall polymers, as well as Gram-negative (Sporomusa sp. MT-2.99 and Pseudomonas fluorescens) and Gram-positive (Paenibacillus sp. MT-2.2) bacteria will be explained by linear combination fitting using the investigated components.
format	article
author	Henry Moll Astrid Barkleit Laura Frost Johannes Raff
author_facet	Henry Moll Astrid Barkleit Laura Frost Johannes Raff
author_sort	Henry Moll
title	Curium(III) speciation in the presence of microbial cell wall components
title_short	Curium(III) speciation in the presence of microbial cell wall components
title_full	Curium(III) speciation in the presence of microbial cell wall components
title_fullStr	Curium(III) speciation in the presence of microbial cell wall components
title_full_unstemmed	Curium(III) speciation in the presence of microbial cell wall components
title_sort	curium(iii) speciation in the presence of microbial cell wall components
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/fa6b5d3f43db40ceac768ab3a0941a9e
work_keys_str_mv	AT henrymoll curiumiiispeciationinthepresenceofmicrobialcellwallcomponents AT astridbarkleit curiumiiispeciationinthepresenceofmicrobialcellwallcomponents AT laurafrost curiumiiispeciationinthepresenceofmicrobialcellwallcomponents AT johannesraff curiumiiispeciationinthepresenceofmicrobialcellwallcomponents
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Curium(III) speciation in the presence of microbial cell wall components

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