Curium(III) speciation in the presence of microbial cell wall components

Trivalent actinides such as Cm(III) are able to strongly interact with microbes and especially with bacterial cell walls. However, detailed knowledge of the influence of different cell wall components is somewhat lacking. For this investigation, we studied the formation of aqueous Cm(III) complexes...

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Autores principales: Henry Moll, Astrid Barkleit, Laura Frost, Johannes Raff
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Lenguaje:EN
Publicado: Elsevier 2021
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Acceso en línea:https://doaj.org/article/fa6b5d3f43db40ceac768ab3a0941a9e
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spelling oai:doaj.org-article:fa6b5d3f43db40ceac768ab3a0941a9e2021-11-06T04:14:50ZCurium(III) speciation in the presence of microbial cell wall components0147-651310.1016/j.ecoenv.2021.112887https://doaj.org/article/fa6b5d3f43db40ceac768ab3a0941a9e2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321009994https://doaj.org/toc/0147-6513Trivalent actinides such as Cm(III) are able to strongly interact with microbes and especially with bacterial cell walls. However, detailed knowledge of the influence of different cell wall components is somewhat lacking. For this investigation, we studied the formation of aqueous Cm(III) complexes with cell wall components (e.g., lipopolysaccharide, peptidoglycan, and plasma membranes) using time-resolved laser-induced fluorescence spectroscopy (TRLFS). For all systems, two specific Cm(III) complexes with the biomacromolecules were observed as a function of pH. Specifically, Cm(III) was found to bind to phosphate and carboxyl groups present in the structure of the biomacromolecules. Stability constants and luminescence parameters of the specific Cm(III) complexes were determined and are presented. The pH of the surrounding aqueous solution, the plasma membrane concentration, and proteins included in the crude plasma membrane fraction were found to significantly impact the complexation of Cm(III). The Cm(III) luminescence spectra with plasma membranes, cell wall polymers, as well as Gram-negative (Sporomusa sp. MT-2.99 and Pseudomonas fluorescens) and Gram-positive (Paenibacillus sp. MT-2.2) bacteria will be explained by linear combination fitting using the investigated components.Henry MollAstrid BarkleitLaura FrostJohannes RaffElsevierarticleCuriumLipopolysaccharidePeptidoglycanPlasma membraneBacterial cell wallsLuminescence spectroscopyEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112887- (2021)
institution DOAJ
collection DOAJ
language EN
topic Curium
Lipopolysaccharide
Peptidoglycan
Plasma membrane
Bacterial cell walls
Luminescence spectroscopy
Environmental pollution
TD172-193.5
Environmental sciences
GE1-350
spellingShingle Curium
Lipopolysaccharide
Peptidoglycan
Plasma membrane
Bacterial cell walls
Luminescence spectroscopy
Environmental pollution
TD172-193.5
Environmental sciences
GE1-350
Henry Moll
Astrid Barkleit
Laura Frost
Johannes Raff
Curium(III) speciation in the presence of microbial cell wall components
description Trivalent actinides such as Cm(III) are able to strongly interact with microbes and especially with bacterial cell walls. However, detailed knowledge of the influence of different cell wall components is somewhat lacking. For this investigation, we studied the formation of aqueous Cm(III) complexes with cell wall components (e.g., lipopolysaccharide, peptidoglycan, and plasma membranes) using time-resolved laser-induced fluorescence spectroscopy (TRLFS). For all systems, two specific Cm(III) complexes with the biomacromolecules were observed as a function of pH. Specifically, Cm(III) was found to bind to phosphate and carboxyl groups present in the structure of the biomacromolecules. Stability constants and luminescence parameters of the specific Cm(III) complexes were determined and are presented. The pH of the surrounding aqueous solution, the plasma membrane concentration, and proteins included in the crude plasma membrane fraction were found to significantly impact the complexation of Cm(III). The Cm(III) luminescence spectra with plasma membranes, cell wall polymers, as well as Gram-negative (Sporomusa sp. MT-2.99 and Pseudomonas fluorescens) and Gram-positive (Paenibacillus sp. MT-2.2) bacteria will be explained by linear combination fitting using the investigated components.
format article
author Henry Moll
Astrid Barkleit
Laura Frost
Johannes Raff
author_facet Henry Moll
Astrid Barkleit
Laura Frost
Johannes Raff
author_sort Henry Moll
title Curium(III) speciation in the presence of microbial cell wall components
title_short Curium(III) speciation in the presence of microbial cell wall components
title_full Curium(III) speciation in the presence of microbial cell wall components
title_fullStr Curium(III) speciation in the presence of microbial cell wall components
title_full_unstemmed Curium(III) speciation in the presence of microbial cell wall components
title_sort curium(iii) speciation in the presence of microbial cell wall components
publisher Elsevier
publishDate 2021
url https://doaj.org/article/fa6b5d3f43db40ceac768ab3a0941a9e
work_keys_str_mv AT henrymoll curiumiiispeciationinthepresenceofmicrobialcellwallcomponents
AT astridbarkleit curiumiiispeciationinthepresenceofmicrobialcellwallcomponents
AT laurafrost curiumiiispeciationinthepresenceofmicrobialcellwallcomponents
AT johannesraff curiumiiispeciationinthepresenceofmicrobialcellwallcomponents
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