Variation of Triterpenic Acids in 12 Wild Syzygium formosum and Anti-Inflammation Activity on Human Keratinocyte HaCaT

Syzygium formosum (Wall.) Masam leaf is known as a Vietnamese traditional herbal medicine used to treat atopic dermatitis and stomach ulcers. Recently, its potent anti-allergic effects were reported with possible active compounds analysis. Here, we collected S. formosum&l...

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Autores principales:	Hyun-ah Park, Mi Yoon Kim, Nan-Young Lee, Jaeyoon Lim, Kyu-been Park, Chang-Kyu Lee, Van Dao Nguyen, Jaehan Kim, Jong-Tae Park, Jong-Il Park
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	anti-inflammation secondary metabolite skin cell <i>Syzygium formosum</i> triterpenic acid Botany QK1-989
Acceso en línea:	https://doaj.org/article/fa70044a826e4cbfbfa8af6826416063
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spelling	oai:doaj.org-article:fa70044a826e4cbfbfa8af68264160632021-11-25T18:46:30ZVariation of Triterpenic Acids in 12 Wild <i>Syzygium formosum</i> and Anti-Inflammation Activity on Human Keratinocyte HaCaT10.3390/plants101124282223-7747https://doaj.org/article/fa70044a826e4cbfbfa8af68264160632021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2428https://doaj.org/toc/2223-7747<i>Syzygium formosum</i> (Wall.) Masam leaf is known as a Vietnamese traditional herbal medicine used to treat atopic dermatitis and stomach ulcers. Recently, its potent anti-allergic effects were reported with possible active compounds analysis. Here, we collected <i>S. formosum</i> leaves from 12 wild trees and compared compositions of triterpenic acids (TA) with <i>Centella asiatica</i>. Anti-inflammatory activities of <i>S. formosum</i> leaf extract (SFLE) was compared with <i>C. asiatica</i> extract (CAE) using human keratinocyte, HaCaT. In this study, up to seven TAs were identified in SFLE, while only madecassic and asiatic acids were detected in the CAE. Total TA content varied among SFLE, but asiatic, corosolic, and betulinic acids were the major components. Surprisingly, wild tree sample 12 (S12) contained total TA of 27.2 mg/g dry-leaves that was 5-fold greater than that in the <i>C. asiatica</i> sample, and S4 had the highest content of asiatic acid (12.6 mg/g dry-leaves) that accounted for 50% of the total TA. S4 and S12 showed more than 3-fold higher anti-oxidative power than the CAE. In the UVB irradiation model, S4 and S12 (5 μg/mL) strongly repressed mRNA levels of pro-inflammatory cytokines (IL-1β, IL-6, and IL-8) and COX-2, while the CAE at the same condition showed moderate or weak repression. The difference in anti-inflammation effects between the SFLE and the CAE was also confirmed by protein quantifications. Taken together, SFLE has great potentials as a new cosmeceutical ingredient with a higher amount of skin-active phytochemicals.Hyun-ah ParkMi Yoon KimNan-Young LeeJaeyoon LimKyu-been ParkChang-Kyu LeeVan Dao NguyenJaehan KimJong-Tae ParkJong-Il ParkMDPI AGarticleanti-inflammationsecondary metaboliteskin cell<i>Syzygium formosum</i>triterpenic acidBotanyQK1-989ENPlants, Vol 10, Iss 2428, p 2428 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anti-inflammation secondary metabolite skin cell <i>Syzygium formosum</i> triterpenic acid Botany QK1-989
spellingShingle	anti-inflammation secondary metabolite skin cell <i>Syzygium formosum</i> triterpenic acid Botany QK1-989 Hyun-ah Park Mi Yoon Kim Nan-Young Lee Jaeyoon Lim Kyu-been Park Chang-Kyu Lee Van Dao Nguyen Jaehan Kim Jong-Tae Park Jong-Il Park Variation of Triterpenic Acids in 12 Wild <i>Syzygium formosum</i> and Anti-Inflammation Activity on Human Keratinocyte HaCaT
description	<i>Syzygium formosum</i> (Wall.) Masam leaf is known as a Vietnamese traditional herbal medicine used to treat atopic dermatitis and stomach ulcers. Recently, its potent anti-allergic effects were reported with possible active compounds analysis. Here, we collected <i>S. formosum</i> leaves from 12 wild trees and compared compositions of triterpenic acids (TA) with <i>Centella asiatica</i>. Anti-inflammatory activities of <i>S. formosum</i> leaf extract (SFLE) was compared with <i>C. asiatica</i> extract (CAE) using human keratinocyte, HaCaT. In this study, up to seven TAs were identified in SFLE, while only madecassic and asiatic acids were detected in the CAE. Total TA content varied among SFLE, but asiatic, corosolic, and betulinic acids were the major components. Surprisingly, wild tree sample 12 (S12) contained total TA of 27.2 mg/g dry-leaves that was 5-fold greater than that in the <i>C. asiatica</i> sample, and S4 had the highest content of asiatic acid (12.6 mg/g dry-leaves) that accounted for 50% of the total TA. S4 and S12 showed more than 3-fold higher anti-oxidative power than the CAE. In the UVB irradiation model, S4 and S12 (5 μg/mL) strongly repressed mRNA levels of pro-inflammatory cytokines (IL-1β, IL-6, and IL-8) and COX-2, while the CAE at the same condition showed moderate or weak repression. The difference in anti-inflammation effects between the SFLE and the CAE was also confirmed by protein quantifications. Taken together, SFLE has great potentials as a new cosmeceutical ingredient with a higher amount of skin-active phytochemicals.
format	article
author	Hyun-ah Park Mi Yoon Kim Nan-Young Lee Jaeyoon Lim Kyu-been Park Chang-Kyu Lee Van Dao Nguyen Jaehan Kim Jong-Tae Park Jong-Il Park
author_facet	Hyun-ah Park Mi Yoon Kim Nan-Young Lee Jaeyoon Lim Kyu-been Park Chang-Kyu Lee Van Dao Nguyen Jaehan Kim Jong-Tae Park Jong-Il Park
author_sort	Hyun-ah Park
title	Variation of Triterpenic Acids in 12 Wild <i>Syzygium formosum</i> and Anti-Inflammation Activity on Human Keratinocyte HaCaT
title_short	Variation of Triterpenic Acids in 12 Wild <i>Syzygium formosum</i> and Anti-Inflammation Activity on Human Keratinocyte HaCaT
title_full	Variation of Triterpenic Acids in 12 Wild <i>Syzygium formosum</i> and Anti-Inflammation Activity on Human Keratinocyte HaCaT
title_fullStr	Variation of Triterpenic Acids in 12 Wild <i>Syzygium formosum</i> and Anti-Inflammation Activity on Human Keratinocyte HaCaT
title_full_unstemmed	Variation of Triterpenic Acids in 12 Wild <i>Syzygium formosum</i> and Anti-Inflammation Activity on Human Keratinocyte HaCaT
title_sort	variation of triterpenic acids in 12 wild <i>syzygium formosum</i> and anti-inflammation activity on human keratinocyte hacat
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fa70044a826e4cbfbfa8af6826416063
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Variation of Triterpenic Acids in 12 Wild <i>Syzygium formosum</i> and Anti-Inflammation Activity on Human Keratinocyte HaCaT

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